Natural medicine-derived suppressor of IL-33 gene expression correlated with eosinophilia

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We observed the close relationship between levels of IL-33 mRNA in nasal mucosa and blood eosinophil in pollinosis patients. We succeeded in finding Swiss3T3 cells in which IL-33 mRNA level was up-regulated by the stimulation of PMA. Then we observed that PMA-induced up-regulation of IL-33 mRNA was strongly suppressed by the extract of Kujin, an anti-allergic Kampo medicine. (-)Maackiain, an inhibitor of PKC-delta from Kujin revealed to be a suppressor. Up-regulation of IL-33 mRNA was also suppressed by either rottlerin, a PKC-delta inhibitor, quercetin, another natural PKC-delta inhibitor or 17-AAG, a suppressor of heat shock protein-90. IL-33 mRNA up-regulation was suppressed by U0126, a MEK inhibitor, but not by DPQ, a PARP-1 inhibitor.