Production of hypoxic adaptation model in rat

所有实验（杂交、妊娠、护理、运动）均在无菌缺氧室进行。4周龄时，将Wistar始祖组（PI）雌雄大鼠随机分为：雄性大鼠缺氧运动组（MHE）、缺氧久坐组（MHS）。男性和女性锻炼组被允许自由使用跑步轮。使用每天读取的机械计数器记录车轮的转数（1.16米，周长）。选取8周内总跑动距离最高的雌雄大鼠。选取的雌雄大鼠进行杂交。在出生后第21天与母鼠分离（第一组为高海拔本地大鼠；F1）。雄性F1大鼠随机分为MHE-F1组、MHS-F1组和常氧久坐低海拔土生大鼠为对照组。18周龄时，用乙醚麻醉P1、F1、F3（第3只高海拔大鼠，第3只高海拔大鼠）、F5（第5只大鼠）和F7（第7只大鼠），取主动脉、脑、心、肝、膈肌、骨骼肌（比目鱼肌和腓肠肌白肌）采血处死。整个大脑分为小脑、额叶、纹状体、海马体四个区域。研究(1)：高原原住民低氧运动组额叶、小脑和纹状体单胺水平较其他组明显降低。这些研究表明，低氧运动降低了全脑单胺水平，并且对缺氧和/或运动的敏感性因大脑区域而异。研究(2)三组间膈肌、比目鱼肌去甲肾上腺素水平无显著差异。这项研究表明，缺氧会引起爱人交感神经反应的减弱，然而，它会引起心脏和腓肠肌白肌反应的增强。这些结果表明，交感神经对长期缺氧的反应随大鼠组织的变化而变化。研究(3)本研究证实，适度缺氧和低氧训练可减轻肝脏DNA损伤，降低肝脏GSH水平和GR活性。这些结果表明，适度的低氧和低氧训练可以降低氧化应激。少

All experiments (hybrid, pregnancy, nurse and exercise) were carried out in the sterile, hypoxia chamber. At the age of 4 weeks, the Progenitor's group (PI) Wistar male and female rats were randomly assigned to the following groups : male rats : hypoxia and exercise (MHE), hypoxia and sedentary (MHS). The male and female exercising groups were allowed free access to the running wheel. The numbers of revolution of the wheel (1.16m, circumference) were recorded by using a mechanical counter that was read daily. Male and female rats who did the highest total amount of running distance during 8 weeks were selected. The selected male and female rats were hybridized. The children (the first of high-altitude native rats ; F1) were separated from their mother after postnatal day 21. The male rats of F1 were randomly divided into three groups : MHE-F1, MHS-F1 and normoxic and sedentary low-altitude native rats, as control group. At the age of 18 weeks, the P1, F1, F3 (the third high-altitude na … More tive rats), F5(the fifth) and F7(the seventh) were anesthetized by ether and sacrificed by sampling of blood from the aorta, and brain, heart, liver, diaphragm, skeletal muscle (soleus and gastrocnemius white muscle). The whole brain was divided into the following four regions : cerebellum, frontal lobe, striatum, hippocampus.Study (1) : Monoamines levels of the hypoxic exercise group in high-altitude native significantly decreased in the frontal lobe, cerebellum and striatum, compare with the other groups. These study suggest that hypoxic exercise decreased monoamine levels in whole brain, and that sensitivity to hypoxia and/or exercise differed according to brain regions.Study (2) No significant differences were found in the norepinephrine levels for the diaphragm and soleus muscle among the three groups. This study clarified that hypoxia elicits a decreased sympathetic response in the lover, However, it cause an increase in the response in the heart and gastrocnemius white muscle. These results suggest that the sympathetic response to long -term hypoxia changes with the rat tissues.Study (3) The present study confirmed that moderate hypoxia and hypoxic training induce attenuate the liver DNA damage and decrease the liver GSH level and GR activity. These results indicate that moderate hypoxia and hypoxic training result in deceased oxidative stress. Less