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Production of hypoxic adaptation model in rat

大鼠缺氧适应模型的制作

基本信息

批准号：
12558002
负责人：
OHKUWA Tetsuo
金额：
$ 7.36万
依托单位：
Nagoya Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

All experiments (hybrid, pregnancy, nurse and exercise) were carried out in the sterile, hypoxia chamber. At the age of 4 weeks, the Progenitor's group (PI) Wistar male and female rats were randomly assigned to the following groups : male rats : hypoxia and exercise (MHE), hypoxia and sedentary (MHS). The male and female exercising groups were allowed free access to the running wheel. The numbers of revolution of the wheel (1.16m, circumference) were recorded by using a mechanical counter that was read daily. Male and female rats who did the highest total amount of running distance during 8 weeks were selected. The selected male and female rats were hybridized. The children (the first of high-altitude native rats ; F1) were separated from their mother after postnatal day 21. The male rats of F1 were randomly divided into three groups : MHE-F1, MHS-F1 and normoxic and sedentary low-altitude native rats, as control group. At the age of 18 weeks, the P1, F1, F3 (the third high-altitude na … More tive rats), F5(the fifth) and F7(the seventh) were anesthetized by ether and sacrificed by sampling of blood from the aorta, and brain, heart, liver, diaphragm, skeletal muscle (soleus and gastrocnemius white muscle). The whole brain was divided into the following four regions : cerebellum, frontal lobe, striatum, hippocampus.Study (1) : Monoamines levels of the hypoxic exercise group in high-altitude native significantly decreased in the frontal lobe, cerebellum and striatum, compare with the other groups. These study suggest that hypoxic exercise decreased monoamine levels in whole brain, and that sensitivity to hypoxia and/or exercise differed according to brain regions.Study (2) No significant differences were found in the norepinephrine levels for the diaphragm and soleus muscle among the three groups. This study clarified that hypoxia elicits a decreased sympathetic response in the lover, However, it cause an increase in the response in the heart and gastrocnemius white muscle. These results suggest that the sympathetic response to long -term hypoxia changes with the rat tissues.Study (3) The present study confirmed that moderate hypoxia and hypoxic training induce attenuate the liver DNA damage and decrease the liver GSH level and GR activity. These results indicate that moderate hypoxia and hypoxic training result in deceased oxidative stress. Less

所有实验（杂交、妊娠、哺乳和运动）均在无菌低氧室中进行。在4周龄时，将雄性和雌性Wistar大鼠随机分为以下组：雄性大鼠：缺氧和运动组（MHE），缺氧和久坐组（MHS）。男性和女性运动组被允许自由进入跑步轮。使用机械计数器记录车轮的转数（1.16 m，周长），每天读取。选择在8周内完成最高总跑步距离的雄性和雌性大鼠。将选择的雄性和雌性大鼠杂交。出生后第21天，将儿童（第一个高海拔本地大鼠; F1）与母亲分开。将F1代雄性大鼠随机分为MHE-F1、MHS-F1组和常氧安静低海拔本地大鼠对照组。在18周龄时，P1、F1、F3（第三代高海拔动物） ...更多信息用乙醚麻醉F5（第5只）和F7（第7只），并从主动脉、脑、心脏、肝脏、膈肌、骨骼肌（比目鱼肌和腓肠肌白色肌）取样处死。研究（1）：高原低氧运动组大鼠额叶、小脑和纹状体的单胺类神经递质水平明显低于其他各组。研究结果表明，低氧运动可降低全脑单胺水平，不同脑区对低氧和/或运动的敏感性不同。研究（2）三组大鼠膈肌和比目鱼肌去甲肾上腺素水平无显著差异。本研究阐明了低氧可使情人交感神经反应减弱，但可使心脏和腓肠肌白色肌的反应增强。研究（3）本研究证实，中度低氧和低氧训练可减轻大鼠肝脏DNA损伤，降低肝脏GSH水平和GR活性。这些结果表明，中度低氧和低氧训练导致氧化应激下降。少