Pathological and epidemiologic study on nasal NK/T-cell lymphoma

鼻腔NK/T细胞淋巴瘤的病理和流行病学研究

基本信息

  • 批准号:
    12576004
  • 负责人:
  • 金额:
    $ 9.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

Gene mutation analysis(1) Mutations of p53 gene(exon5-8) were analyzed in cases with nasal Nk/T-cell l lymphoma(NKTCL)(19from China(Bejin)and 23 from Japan) with use of polymerase chaln reaction(PCR)-slngle strand conformation polyrnorphism followed by direct sequencing. P53 gene mutations were found in 20 cases(47.6%) with missense mutations in 60% of cases. Mutation frequency in Bejiing(63%) washigher than that in Japan(39%)(2) c-kit gene mutations were examined in 23 cases with nasal NKTCL(14 from Bejiing and a from Japan) by PCR-SSCP analysis. Mutation frequency in China(71.4%) much higher than than that in Japan(22%). Mutated c-kit genes transfected into 293T cell did not generate constitutive activation of c-kit, thus nota gain-of-function mutation(3) p53(exon4-8), c-kit(exon 11, 17), k-ras(exon1, 2), and β-catenin gene(exon3), mutations were examined in 20 cases with nasal NKTCL from northeast dlstrict of China, P53 mutations were found in 40% of cases with significantly lower involvement of exon4 than Japan Mutations of c-kit gene were found in only 5% of cases. Mutations of k-ras and β-catenin were found 5% and 30% of cases, respectively. These findings showed that mutation frequency and pattern of suppressor gene and oncogenes were various by direct, suggestion that presence of different environment factors for developrnent of nasal NK/T cell lymphoma.
基因突变分析(1)应用聚合酶链反应(PCR)-单链构象多态性分析和直接测序技术,对19例中国人和23例日本人鼻NK/T细胞淋巴瘤(NKTCL)p53基因第5 -8外显子进行突变分析。P53基因突变20例(47.6%),错义突变占60%。(2)应用PCR-SSCP方法检测了23例鼻NKTCL患者(14例来自北京,1例来自日本)的c-kit基因突变。中国的突变频率(71.4%)远高于日本(22%)。突变的c-kit基因转染293 T细胞后,c-kit基因不产生组成性激活,因此未发现功能获得性突变(3)p53(exon 4 -8),c-kit(外显子11,17),k-ras对东北地区20例鼻NKTCL患者进行了β-catenin基因(exon 1,2)和β-catenin基因(exon 3)突变检测,P53基因突变率为40%,第4外显子突变率明显低于日本,c-kit基因突变率仅为5%。K-ras和β-catenin突变分别占5%和30%。这些结果表明,鼻NK/T细胞淋巴瘤的发生与不同的环境因素有关,其抑癌基因和癌基因的突变频率和突变方式也不同。

项目成果

期刊论文数量(72)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takakuwa T et al.: "Frequent mutations of Fas gene in nasal NK/T cell lymphoma"Oncogene. 21. 4702-4705 (2002)
Takakuwa T 等人:“鼻 NK/T 细胞淋巴瘤中 Fas 基因的频繁突变”癌基因。
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    0
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Kanno H, Nakatsuka S, Iuchi K, Aczasa K.: "Sequence of cytotoxic T-lymphocyte epitopes in Epstein-Barr virus(EBV) nuclear antigen-3B gene in Japanese population with or without EBV-positive lymphoid malignancies."Int.J Cancer. 88. 626-632 (2000)
Kanno H、Nakatsuka S、Iuchi K、Aczasa K.:“患有或不患有 EBV 阳性淋巴恶性肿瘤的日本人群中 Epstein-Barr 病毒 (EBV) 核抗原 3B 基因中细胞毒性 T 淋巴细胞表位的序列。”Int.J
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    0
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Nakatsuka S, Takakuwa T, Yao M, Tomita Y, Hoshida Y, Nishiu M, Yamaguchi M Nishii K, Yang WI, Aozasa K.: "Hypermethylaticn of DAP-Kinase CPG islands is frequent in B-cell but not in T-cell mallbnancies"Cancer Sci. 94. 87-91 (2003)
Nakatsuka S、Takakuwa T、Yao M、Tomita Y、Hoshida Y、Nishiu M、Yamaguchi M Nishii K、Yang WI、Aozasa K.:“DAP 激酶 CPG 岛的高甲基化在 B 细胞中常见,但在 T 细胞中不常见
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    0
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Takakuwa T,Aozasa K, et al: "Microsatellite instability and k-ras, p53 mutations in thyroid lymphoma."Jpn J Cancer Res. 91. 280-286 (2000)
Takakuwa T、Aozasa K 等人:“甲状腺淋巴瘤中的微卫星不稳定性和 k-ras、p53 突变。”Jpn J Cancer Res。
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    0
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Li T,Aozasa K, et al.: "Mutations of p53 gene in nasal NK/T-cell lymphoma."Lab Invest. 80. 493-499 (2000)
Li T,Aozasa K, et al.:“鼻 NK/T 细胞淋巴瘤中 p53 基因的突变。”Lab Invest。
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AOZASA Katsuyuki其他文献

AOZASA Katsuyuki的其他文献

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{{ truncateString('AOZASA Katsuyuki', 18)}}的其他基金

Study on patho-epidemiology of malignant lymphoma in Japan
日本恶性淋巴瘤病理流行病学研究
  • 批准号:
    16390105
  • 财政年份:
    2004
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Patho-epidemiological study on the nasal NK/T cell lymphoma.
鼻腔NK/T细胞淋巴瘤的病理流行病学研究。
  • 批准号:
    15406013
  • 财政年份:
    2003
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of DNA sequence of immunoglobulin heavy chain variable region gene in lymphomas developing from chronic inflammation
慢性炎症淋巴瘤免疫球蛋白重链可变区基因DNA序列分析
  • 批准号:
    12670159
  • 财政年份:
    2000
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study on modifying factors in the development of EBV-associated lymphomas
EBV相关淋巴瘤发生发展的调节因素研究
  • 批准号:
    09670184
  • 财政年份:
    1997
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathological study on nasal T-cell lymphoma in East Asia
东亚鼻T细胞淋巴瘤的病理学研究
  • 批准号:
    07042005
  • 财政年份:
    1995
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Pathological and epidemiological studies on the pathogenesis of B-cell lymphoma
B细胞淋巴瘤发病机制的病理学和流行病学研究
  • 批准号:
    01015065
  • 财政年份:
    1989
  • 资助金额:
    $ 9.34万
  • 项目类别:
    Grant-in-Aid for Cancer Research
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