Pathological and epidemiologic study on nasal NK/T-cell lymphoma

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Gene mutation analysis(1) Mutations of p53 gene(exon5-8) were analyzed in cases with nasal Nk/T-cell l lymphoma(NKTCL)(19from China(Bejin)and 23 from Japan) with use of polymerase chaln reaction(PCR)-slngle strand conformation polyrnorphism followed by direct sequencing. P53 gene mutations were found in 20 cases(47.6%) with missense mutations in 60% of cases. Mutation frequency in Bejiing(63%) washigher than that in Japan(39%)(2) c-kit gene mutations were examined in 23 cases with nasal NKTCL(14 from Bejiing and a from Japan) by PCR-SSCP analysis. Mutation frequency in China(71.4%) much higher than than that in Japan(22%). Mutated c-kit genes transfected into 293T cell did not generate constitutive activation of c-kit, thus nota gain-of-function mutation(3) p53(exon4-8), c-kit(exon 11, 17), k-ras(exon1, 2), and β-catenin gene(exon3), mutations were examined in 20 cases with nasal NKTCL from northeast dlstrict of China, P53 mutations were found in 40% of cases with significantly lower involvement of exon4 than Japan Mutations of c-kit gene were found in only 5% of cases. Mutations of k-ras and β-catenin were found 5% and 30% of cases, respectively. These findings showed that mutation frequency and pattern of suppressor gene and oncogenes were various by direct, suggestion that presence of different environment factors for developrnent of nasal NK/T cell lymphoma.