HCG β molecules and the glycosyl-transferase gene study of chorionic diseases with high incidence in the South East Asia

东南亚高发绒毛膜疾病的HCG β分子和糖基转移酶基因研究

• 批准号: 12576028
• 负责人: FUKUOKA Hideoki
• 金额: $ 9.28万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12576028/
• 关键词: sialic acid; hCG; sialidase; sugar chain structure; chorionic disease; hyperthyroidism; シリアダーゼ; 絨毛癌

Villous malignant change brings about hydatidiform mole or choriocarcinoma with the change of sugar chain structures and bioactivity of hCG, As In Japan, the cases of these diseases have been decreasing; we have constructed the cooperative research projects, as a counterpart, with the Department of Internal Medicine Mahidol University Medical School, Thai Land. However, the number of the studied cases is not so many. Sugar chain structures had been analyzed by Isoelectrofocussing, sialic acid content, deglycosidase study, and affinity study to lectins. In addition, the gene expression of glycosyl-transferases was analyzed. These diseases are reported to show severe hyperthyroidism. Our cases were, however, divided into hyperthyroidism and not hyperthyroidism groups. Isoelectrofocussing disclosed that the cases with much hCG molecules with neutral regions showed hyperthyroidism and those with strong acidic regions did not show that. Sialidase treatment induced the acidic molecules moving into the neutral region, suggesting the hCG molecules of acidic regions having much sialic acid molecules. Similarly, RT-PCR study confirmed that the villi with much acidic region molecules expressed much expression of sialidase genes.

绒毛恶性变化导致葡萄胎或绒毛膜癌与糖链结构和hCG的生物活性的变化，因为在日本，这些疾病的病例一直在减少;我们已经建立了合作研究项目，作为对应，与内科玛希隆大学医学院，泰国土地。然而，研究的案例数量并不多。通过等电聚焦、唾液酸含量、去糖苷酶研究和凝集素亲和性研究分析了糖链结构。此外，糖基转移酶的基因表达进行了分析。据报道，这些疾病表现为严重的甲状腺功能亢进。然而，我们的病例被分为甲状腺功能亢进组和非甲状腺功能亢进组。等电聚焦显示，hCG分子中性区多的病例表现为甲亢，而酸性区强的病例则不表现甲亢。唾液酸酶处理诱导酸性分子进入中性区，表明酸性区的hCG分子含有较多的唾液酸分子。同样，RT-PCR研究证实具有较多酸性区域分子的绒毛表达较多的唾液酸酶基因。