Grant-in Aid for Scientific Research(C)(2)

科学研究补助金(C)(2)

• 批准号: 14570324
• 负责人: FUKUOKA Hideoki
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570324/
• 关键词: head down tilting bed rest; immobilization; uncoupling bone loss; metabolic turnover of bone; osteocytic osteolysis; Ca-sensing receptor; 不動性骨度減少; 高代謝回転型骨量減少; 骨細胞; スペースフライト

The microgravity will induce bone loss. With young healthy males (n=13, 26.6±3.5) we have executed the 6-degree head down tilting bed rest immobilization for 20 days. We have revealed 2-mechanisms for the bone loss, the coupled metabolic high turnover of osteoclast and osteoblast and another osteolysis without hyperactivity of osteoclast and osteoblast, and over-expression of calcium sensing receptor and cytokine mRNAs in macrophage/peripheral mononuclear cell. In the coupled high turnover group, both activity of osteoclast and osteoblast had increased. Another group did not show any increase of metabolic markers of osteoclast and osteoblast. Comparing the 2 group with pQCT, only the BMD of the cancellous bone decreased in the coupled high turnover group. In another, the BMD of both bones decreased. Theses 2-typed bone loss reaction induced by immobilization should be determined hereditally. When this polymorphism is determined, high and low risk persons in space flight would be clearly divided, and the protective methods for each group for bone loss will be created most effectively.

微重力会导致骨质流失。我们对年轻健康男性（n=13, 26.6±3.5）进行了6度头部向下倾斜卧床休息固定20天。我们揭示了巨噬细胞/外周单核细胞中骨丢失的两种机制，即破骨细胞和成骨细胞的高代谢交替和另一种无破骨细胞和成骨细胞过度活跃的骨溶解，以及钙敏感受体和细胞因子mrna的过度表达。偶联高周转率组破骨细胞和成骨细胞活性均升高。另一组破骨细胞和成骨细胞的代谢标志物未见增加。2组与pQCT比较，耦合高转换组只有松质骨BMD下降。在另一种情况下，两根骨头的骨密度都下降了。固定所致的2型骨质流失反应应由遗传决定。一旦确定了这种多态性，就可以明确地划分出太空飞行中的高、低风险人群，从而最有效地制定出针对每一人群的骨质流失保护措施。

项目成果

R, Wakimizu, Z, Chen, H, Fukuoka, N, Suzuki, J, Nomura.: "Effect of resistance training on changes in lymphocyte subpopulation during head-down-tilt bed rest."Biol Sci Space.. 17. 200 (2003)

R, Wakimizu, Z, Chen, H, Fukuoka, N, Suzuki, J, Nomura.：“阻力训练对低头倾斜卧床休息期间淋巴细胞亚群变化的影响。”Biol Sci Space.. 17. 200 (

H, Akima, J, Ushiyama, J, Kubo, S, Tonosaki, M, Itoh, Y, Kawakami, H, Fukuoka, H, Kanehisa, T, Fukunaga: "Resistance training during un weighting maintains muscle size and function in human calf"Medicine & Science In Sports & Exercise. 655-662 (2003)

H、Akima、J、Ushiyama、J、Kubo、S、Tonosaki、M、Itoh、Y、Kawakami、H、Fukuoka、H、Kanehisa、T、Fukunaga：“未称重期间的阻力训练可维持小腿肌肉的大小和功能

Fujita M, Fukuoka H. et al.: "Estrogen activates cyclin-dependent kinase 4 and 6 through induction of cyclin Din rsat primary osteoblasts"BBRC. 299. 222-228 (2002)

Fujita M、Fukuoka H. 等人：“雌激素通过诱导细胞周期蛋白 Din rsat 原代成骨细胞激活细胞周期蛋白依赖性激酶 4 和 6”BBRC。

H, Akima.J, Ushiyama, J, Kubo, S, Tomosaki, H, fukuoka, T, Fukunaga: "Effect of resistance training during short-duration of unweighting on functional magnetic resonance imaging of muscle."J Applied Physiol. 93. 672-685 (2002)

H，Akima.J，Ushiyama，J，Kubo，S，Tomosaki，H，fukuoka，T，Fukunaga：“短期减重过程中阻力训练对肌肉功能磁共振成像的影响。”J Applied Physiol。

H, Akima.J, Ushiyama, J, Kubo, S, Tomosaki, M.Ito, Y, Kawakami, H, Fukuoka, H, Kanehisa, T, Fukunaga: "Resistance training during unweighting maintains muscle size and function in human calf."Med. Sci. Sports Exer. 35. 655-662 (2003)

H, Akima.J, Ushiyama, J, Kubo, S, Tomosaki, M.Ito, Y, Kawakami, H, Fukuoka, H, Kanehisa, T, Fukunaga：“减重过程中的阻力训练可以维持小腿肌肉的大小和功能。