Gene expression of Vit D conversion eyzymes and Ca metaboism in the feto-placental system

胎儿胎盘系统中维生素 D 转化酶和钙代谢的基因表达

• 批准号: 01570934
• 负责人: FUKUOKA Hideoki
• 金额: $ 1.41万
• 依托单位: Faculty of Medicine, University of Tokyo (1990-1991)Kagawa Medical School (1989)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570934/
• 关键词: Calcium metabolism; Feto-placental unit; 1-alpha-hydroxylase; 25-hydroxyvitamin D 24R-hydroxylase; Vitamin D conversion enzyme; Vitamin D; 活性型ビタミンD; ビタミンD転換酵素; 胎盤IUGR(子宮内胎児発育不良)

Calcium metabolism is one of the most interesting areas in the endocrinology. Much amount of research have been done to clarify it in the molecular levels. However, the regulating mechanisms in the feto-placental systems are to be clarified. In addition, the mechanism of gene expression of Vit D conversion enzymes in the fetus is a little different from the adult organs. So our research project consisted with (1) studying chronologically the enzymatic characterization of 1-alpha-hydroxylase and 24R-hydroxylase in the mitochondria in the human placenta, (2) analyzing the 1-alpha-hydroxylase and 24R-hydroxylase in the fetal brain, lung, kidney, which has been thought to be exist only in the renal tubules.We have found that the biologically active vitamin D was synthesized in the placenta and is one of the important substances regulating the fetal growth. The activity of 24R-hydroxylase appears around pregnancy 20 weeks and slightly increases until partrition. In the placenta of severe type of IUGR (intrauterine growth retardation), however, only 24R-hydroxylase except 1-alpha-hydroxylase was found. We suspect that the gene expression of this enzyme might be inhibited. The activity of rabbit fetus and neonate, and that of 1-alpha-hydroxylase was also found in the brain, placenta, kidney. In the brain it increased transiently and disappeared after the age of the neonate. These new finding suspects that the vitamin D conversion enzyme system might consist with the two types of the adult and the fetus, appearing transiently. We are trying to characterize these fetal systems, especially of 1-alpha-hydroxylase.

钙代谢是内分泌学中最有趣的领域之一。大量的研究已经完成，以澄清它在分子水平上。然而，胎儿-胎盘系统的调节机制尚待阐明。此外，胎儿体内维生素D转化酶基因表达的机制与成人器官略有不同。因此，我们的研究项目包括：（1）按时间顺序研究人胎盘线粒体中1-α-羟化酶和24 R-羟化酶的酶学特性，（2）分析胎儿脑、肺、肾、我们发现，具有生物活性的维生素D是在胎盘中合成的，是调节胎儿生长发育的重要物质之一。24 R-羟化酶的活性在妊娠20周左右出现，并在分娩前略有增加。而在重度IUGR（intrauterine growth retardation）胎盘中，除1-α-羟化酶外，仅发现24 R-羟化酶，我们怀疑该酶的基因表达可能受到抑制。胎兔和新生兔脑、胎盘、肾中也有1-α-羟化酶活性。在脑中，它短暂增加，并在新生儿年龄后消失。这些新发现推测维生素D转换酶系统可能与成人和胎儿两种类型一致，只是短暂出现。我们正试图描述这些胎儿系统，特别是1-α-羟化酶。

项目成果

H. Fukuoka, T. Jimbo: "Gene expression and processing of peptide hormones -LH and FSH-" Jpn J Clin Med. 47. 2204-2212 (1989)

H. Fukuoka，T. Jimbo：“肽激素 -LH 和 FSH - 的基因表达和加工”Jpn J Clin Med。

H. Fukuoka, I. Kobayashi et al.: "Biochemical characterization of placental vitamin D conversion enzymes in the placenta of epileptic mother treated with of anticonvulsant." Jpn J Maternal Health. 31. 569-570 (1990)

H. Fukuoka、I. Kobayashi 等人：“用抗惊厥药治疗的癫痫母亲胎盘中胎盘维生素 D 转化酶的生化特征。”

H.Fukuoka,R.Hamamoto,M.Higurashi;: "Microheterogeneity of human prolactin in the circualtion and in the amniotic fluid." in Hormon Research in press.

H.Fukuoka，R.Hamamoto，M.Higurashi；：“人催乳素在循环系统和羊水中的微异质性。”

福岡 秀興 その他: "抗テンカン剤服用妊娠における胎盤性ビタミンD転換酵素活性の生化学的分析" 母性衛生. 31. 569-570 (1990)

Hideoki Fukuoka 等：“服用抗体温药物的妊娠期胎盘维生素 D 转化酶活性的生化分析”《孕产妇卫生》31. 569-570 (1990)。

福岡 秀興,神保 利春: "ペプチドホルモン前駆体遺伝子の構造,発現調節と前駆体のプロセッシングー性腺刺激ホルモン(LH,FSH)ー" 日本臨床. 47. 2204-2212 (1989)

Hideoki Fukuoka，Toshiharu Jimbo：“肽激素前体基因的结构、表达调节和前体的加工 - 促性腺激素（LH、FSH） -”日本临床研究 47. 2204-2212 (1989)。