Phosphorylation and dephosphorylation reactions in stomatal guard cells of blue light signaling

蓝光信号传导气孔保卫细胞的磷酸化和去磷酸化反应

• 批准号: 13440243
• 负责人: SHIMAZAKI Ken-ichiro
• 金额: $ 9.34万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440243/
• 关键词: Stomata; Proton pump; Guard cells; 14-3-3_protein; Protein phosphorylation; Protein dephosphorylation; Abscisic acid; Blue light response; ABA; フォトトロピン

Protein phosphorylation and dephosphoryaltion are involved in the signal transduction pathways of stomatal guard cells. Since the guard cells can respond to both blue light and phytohormone abscisic acid, we investigated these responses using guard cell protoplasts.The blue light receptor is autophosphorylated in response to blue light, and a 14-3-3 protein was found to bind to phosphorylated serine in phototropin. The site is identified to be localized between LOV1 and LOV2.We found that ABA inhibits this blue light signaling pathways in stomatal guard cells. ABA inhibited the phosphorylation of the plasma membrane H^+-ATPase and inhibited the H^+ pumping from guard cells. We also found that ABA inhibited the ATP hydrolytic activity of the plasma membrane H^+-ATPase.

蛋白质磷酸化和去磷酸化参与气孔保卫细胞的信号转导途径。由于保卫细胞对蓝光和植物激素脱落酸都有反应，我们用保卫细胞原生质体研究了这些反应，发现蓝光受体对蓝光的反应是自磷酸化的，并且发现一个14-3-3蛋白与光促素中磷酸化的丝氨酸结合。我们发现阿坝抑制气孔保卫细胞的蓝光信号通路。阿坝抑制质膜H^+-ATPase的磷酸化，抑制保卫细胞的H^+泵出。阿坝抑制了质膜H^+-ATPase的ATP水解活性。

项目成果

Li et al.: "Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase"Nature. 418. 793-797 (2002)

Li等人：“脱落酸激活蛋白激酶对RNA结合蛋白的调节”自然。

Misumi Tominaga: "Gurd-cell chloroplasts provides ATP required for H+ pumping in the plasma membrane and stomatal opening"Plant & Cell Physiology. 42. 795-802 (2001)

Misumi Tominaga：“根细胞叶绿体提供质膜和气孔开口中 H 泵送所需的 ATP”植物

島崎研一郎: "植物の光センシング"秀潤社. 192 (2001)

Kenichiro Shimazaki：“植物的光传感”Shujunsha 192（2001）。

Yamashita et al.: "Evidence for nucleotidedependent passive H^+ transport protein in the plasma membrane of barley roots."Plant Cell Physiol. 44. 55-61 (2003)

Yamashita 等人：“大麦根质膜中核苷酸依赖性被动 H+转运蛋白的证据。”植物细胞生理学。

Kinoshita et al.: "Blue-light-and phosphorylation-dependent binding of a 14-3-3 protein to phototropins in stomatal guard cells of broad bean."Plant Physiol. 133. 1453-1463 (2003)

Kinoshita 等人：“14-3-3 蛋白与蚕豆气孔保卫细胞中的向光素的蓝光和磷酸化依赖性结合。”植物生理学。