Molecular Mechanisms of Enhancement of Plant Growth by phototropins.

向光素促进植物生长的分子机制。

基本信息

批准号：
16207003
负责人：
SHIMAZAKI Ken-ichiro
金额：
$ 31.03万
依托单位：
KYUSHU UNIVERCITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Appropriate leaf positioning is essential for optimizing photosynthesis and plant growth. We show here the regulation of leaf positioning under blue light stimuli. When one-week-old Arabidopsis seedlings grown under white light were transferred to red light (25 μmol m-2 s-1) for 5 days, new petioles that appeared were almost horizontal and their leaves were curled and slanted downward. However, when a weak blue light from above (0.1 μmol m-2 s-1) was superimposed on red light, the new petioles grew obliquely upward and the leaves were flat and horizontal. The leaf positioning required both phototropin1 (phot1) and non-phototropic hypocotyl 3 (NPH3), and resulted in enhanced plant growth. In an nph3 mutant, neither optimal leaf positioning nor leaf flattening by blue light was found, and blue light-induced growth enhancement was drastically reduced. When blue light was increased from 0.1 μmol m-2 s-1 to 5 μmol m-2 s-1,normal leaf positioning and leaf flattening were induced in both phot1 and nph3 mutants, suggesting that phot2 signaling became functional and that the signaling was independent of phot1 and NPH3 in these responses. When plants were irradiated with blue light (0.1 μmol m-2 s-1) from the side and red light from above, the new leaves became oriented toward the source of blue light. We showed the tissue expression of NPH3 and its plasma membrane localization via the coiled-coil domain and the C-terminus region. We conclude that NPH3-mediated phototropin signaling optimizes the efficiency of light perception by inducing both optimal leaf positioning and leaf flattening, and enhances plant growth.

适当的叶子定位对于优化光合作用和植物生长至关重要。我们在这里显示了在蓝光刺激下叶片定位的调节。当将在白光下生长的一周大的拟南芥幼苗被转移到红光（25μmolM-2 S-1）中5天时，出现的新叶柄几乎是水平的，其叶子被卷曲并倾斜。但是，当从上方（0.1μmolM-2 S-1）叠加在红光下的蓝色弱蓝光时，新的叶柄会倾斜地向上生长，叶子平坦且水平。叶片定位需要光photropin1（Phot1）和非光质下邻苯基3（NPH3），并导致植物生长增强。在NPH3突变体中，既没有发现最佳的叶片定位，也没有发现蓝光扁平的叶片，并且蓝光诱导的生长增强大幅降低。当蓝光从0.1μmolM-2 S-1增加到5μmolM-2 S-1时，在Photo1和NPH3突变体中诱导了正常的叶片定位和叶片变平，这表明Photo2信号传导变得功能性，并且该信号在这些响应中与Photo1和NPH3无关。当植物从侧面用蓝光（0.1μmolM-2 S-1）和从上方的红光照射时，新叶子就朝向蓝光的来源。我们通过盘绕圈结构域和C末端区域展示了NPH3及其质膜定位的组织表达。我们包括NPH3介导的光蛋白信号传导通过诱导的最佳叶片定位和叶片变平并增强植物的生长来优化光感知的效率。

项目成果

期刊论文数量（30）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Isolation of a Protein Interacting with Vfphotla in Guard Cells of Vicia faba.

蚕豆保卫细胞中与 Vfphotla 相互作用的蛋白质的分离。

DOI：
发表时间：
2005
期刊：
Plant Physiol 138
影响因子：
0
作者：
Nae FA;Saito N;Hozumi A;Takai O;Emi et al.
通讯作者：
Emi et al.

Light Sensing in Plants

植物的光感应

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Simazaki;K.;Wada et al.
通讯作者：
Wada et al.

気孔の開口を駆動する細胞膜H^+-ATPase

驱动气孔打开的细胞膜 H^+-ATP 酶

DOI：
发表时间：
2006
期刊：
蛋白質・核酸・酵素 51
影响因子：
0
作者：
木下俊則;島崎研一郎
通讯作者：
島崎研一郎

Nitric oxide inhibits blue light-specific stomatal opening via abscisic acid signaling pathways in Vicia guard cells.

DOI：
10.1093/pcp/pcm039
发表时间：
2007-05
期刊：
Plant & cell physiology
影响因子：
4.9
作者：
Xiao Zhang;A. Takemiya;T. Kinoshita;K. Shimazaki
通讯作者：
Xiao Zhang;A. Takemiya;T. Kinoshita;K. Shimazaki

Leaf positioning of Arabidopsis in responese to blue light.

拟南芥响应蓝光的叶片定位。

DOI：
发表时间：
2007
期刊：
Mol. Plant (in press)
影响因子：
0
作者：
Atsushi Ikeda;Keisuke Itoh;Tatsuya Suzuki;Masao Aida;Yasuhiko Fujii;Toshiaki Mitsugashira;Mitsuo Hara;Masaki Ozawa;Inoue et al.
通讯作者：
Inoue et al.