Subtyping of diffuse large B cell lymphomas by molecules highly expressed on germinal center B cells.

弥漫性大B细胞淋巴瘤在淋巴瘤分化中属于废纸篓型，为了对其进行细分，我们打算添加一些可靠的分子来参与这种细分。我们发现在生发型B细胞型弥漫性大B细胞淋巴瘤中只表达三个分子：U50HG、蛋氨酸氨基肽酶2 （MetAP2）和胆固醇酯转移蛋白（CET）。U50HG是一种蛋白质非编码的snoRNA宿主基因，我们发现其转录本只在生发中心高表达。另外两个分子metap2a CET在生发中心B细胞及其肿瘤对应细胞中被其特异性抗体强烈检测到，而在其他B细胞和t细胞淋巴瘤中则非常弱。这些结果表明，这三个基因产物对肿瘤B淋巴细胞细分为生发中心型和其他（可能被激活的）B细胞型非常有用。此外，我们发现一个新的癌基因pim-1在某种表现出Bc16/Pim1染色体易位的B细胞淋巴瘤上形成显性阴性嵌合转录物。

To subdivide the diffuse large B cell lymphoma which is a waste-basket type category in lymphoma differentiation, we intended to add some reliable molecules that contribute in such subdivision. We could identify three molecules that are expressed in diffuse large B cell lymphomas of germinal cents B cell type exclusively : U50HG, methionine aminopeptidase 2 (MetAP2) and cholesterine ester transfer protein (CET). In case of U50HG that is a protein non-coding snoRNA host gene, we found its transcript to be highly expressed in germinal center exclusively. Other two molecules, MetAP2 a CET were detected in germinal center B cells and their neoplastic counterparts intensely by their specific antibodies while very weakly on other B-and T-cell lymphomas. Those results suggest that the three gene products are very much useful for the subdivision of neoplastic B lymphocytes into germinal center type and other (may be activated B cell) type. Furthermore, we found a novel oncogene pim-1 to form a dominant negative type chimeric transcript on a certain B cell lymphoma that exhibit Bc16/Pim1 chromosomal translocation.