The study on functions on the membrane type collectm CL-P1 in vascular cells.

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Collectins are a family of C type lectins which have collagen-like sequences and carbohydrate recognition domains (CRD). The scavenger receptors type A and MARCO are classical type scavenger receptors which have internal collagen-like domains. Here, we found a new scavenger receptor which is a membrane type collectin from placenta (collectin placenta 1=CL-P1) that has a typical collectin collagen-like domain and a CRD. To elucidate the functions in CL-P1 in animal body is our purpose. In 3 years, we finished seven projects below,1.Cloning of CL-P1 cDNA in human, mouse, rat, zebra fish, xenopus.2.Establishment of CL-P1 permanent expression cells.3.Production of soluble recombinant CL-P1 and mutant one having deletion or point mutation.4.Discovery of binding protein against CL-P1.5.Analyses oxidized LDLs after having different oxidation treatment.6.Study on the promoter activity in CL-P1 5'-franking region.7.Study on the kinetics of CL-P1 after several physiological treatments.