Study of analysis for vascular damage due to embolism and inflammation

CL-P1是Ohtani于2001年发现的一种新的聚集蛋白。它在小鼠组织中的血管内皮细胞中表达，并且也在血管内皮细胞系中发现。本研究建立了抗CL-P1的抗体，以研究其生物学功能。我们用重组CL-P1蛋白免疫小鼠，制备了单克隆抗体杂交瘤。建立了几种单克隆抗体，并分为两组或三组。我们尝试用上述单克隆抗体建立检测CL-P1的分析方法。我们做了几个原型检测系统，但我们不能得到很好的结果，因为较小的CL-P1体积。现在我们开始使用多克隆抗体建立一种新的分析系统。

CL-P1 is a new collectin which was found by Ohtani at 2001. It was expressed in vascular endothelial cells in murine tissues and also found in vascular endothelial cell lines. Here we established the antibody against above CL-P1 to investigate their biological functions. We have immunized mice with recombinant CL-P1 protein and made hybridoma for monoclonal antibodies. Several monoclonal antibodies were established and were divided to two or three groups. We tried to make the analysis method to detect CL-P1 using above monoclonal antibodies. We made several prototype assay systems but we could not get a good result because of less CL-P1 volume. Now we began to make a new analysis system using polyclonal antibodies.