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The relationship between the stress and serum mannan-binding lectin.

应激与血清甘露聚糖结合凝集素之间的关系。

基本信息

批准号：
09672356
负责人：
WAKAMIYA Nobutaka
金额：
$ 1.92万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672356/
关键词：
COLLECTIN LECTIN VIRUS COMPLEMENT STRESS MICROBES 遺伝子クローニング

项目摘要

Mannan-binding lectin (MBL) is a C-type serum lectin which is believed to play an important role in innate immunity.It is one of the collectin family which are characterized by having a collagen-like sequence and a carbohydrate recognition domain.Collectin can bind to sugar determinants of several microorganisms, neutralize them and inhibit infection by the of complement activation through the lectin pathway and opsonization by collectin receptors.First we tried to isolate collectin genes in several animal and establish the production system of collectin in E.coli and CHO cells.Next to reveal the role of collectin under the stress condition, we investigated the serum concentration from persons who were given with/without stress.(1) We isolated and characterized the cDNA encoding bovine and rabbit MBP (Kawai et al., Gene. 1997, Glycobiology. 1998).(2) We established the high and effective production system of bovine conglutinin and human SP-D and MBP (Eda et al, Biochem. J.1997, Biosci. Biotech. Bioch., 1998., Suzuki, et al., Biochem. Biophys. Res. Commun., 1997, Ohtani et al., J.Immunol. Methods, 1999).(3) We measured the serum concentration and gene allele of MBP in persons from different groups.We found only the gene mutation of codon 54 in all Japanese population (over 700 samples).Fulminant Hepatitis patient (death group) was seen in low MBP level, in comparison with the patient (survived group).

甘露聚糖结合凝集素（Mannan-binding lectin，MBL）是一种C型血清凝集素，在天然免疫中起重要作用，属于凝集素家族，具有胶原样序列和糖识别结构域，能与多种微生物的糖决定簇结合，通过凝集素途径激活补体和凝集素受体的调理作用来中和它们并抑制感染。首先，我们试图分离了几种动物的胶原凝集素基因，建立了大肠杆菌和CHO细胞的胶原凝集素生产系统，并对应激前后血清中胶原凝集素的浓度进行了测定，以揭示胶原凝集素在应激条件下的作用。(1)我们分离并表征了编码牛和兔MBP的cDNA（Kawai等人，基因1997，糖生物学。1998年）。(2)我们建立了牛促凝素和人SP-D和MBP的高效生产系统（Eda等，Biochem.J.1997，Biosci. Biotech.生物，1998., Suzuki等人，生物化学、生物物理Res. Commun.，1997年，Ohtani等人，Immunol. Methods，1999）。(3)我们检测了不同人群血清MBP浓度和基因等位基因，在日本所有人群（700多例样本）中仅发现第54位密码子的基因突变，重型肝炎患者（死亡组）与患者（存活组）相比，MBP水平较低。