by using DNA microarray

牙周组织作为一个单一的单位发挥作用，尽管它的每个组成部分都有不同的功能、组成和结缔组织结构。因此，利用基因组技术研究牙周组织各细胞的分子功能具有重要意义。在本研究中，我们针对牙周疾病的具体过程，构建了基于龈上皮细胞、龈成纤维细胞、牙周韧带衍生细胞和成骨细胞等牙周组织细胞类型的细胞培养体系，并利用生物信息学技术进行了大规模的基因表达监测分析。(1)人牙龈上皮。从人正常牙龈组织中原代培养牙龈细胞和牙龈成纤维细胞，利用DNA芯片技术分析其基因表达差异。(2)用机械应力、牙周病原菌LPS和Emdogain刺激牙周膜源性细胞，观察其基因表达变化。(3)用低能级二极管激光照射成骨细胞，构建减法基因库，分析转录水平增强的基因，并进一步采用DNA芯片技术进行分析。(4)利用GeneChip技术将人骨髓间充质干细胞诱导成骨细胞，并监测其基因表达水平。(5)用咖啡因刺激人胎盘滋养细胞，利用DNA芯片检测基因表达水平，并利用RT-PCR对咖啡因喂养的孕鼠胎盘进行分析。采用DNA芯片技术研究衰老对小鼠颌下腺基因表达水平的影响。我们进行这项研究的原因是：牙周病引起的低出生婴儿；唾液成分有力地支持牙周组织的健康。通过实验模型在牙周组织细胞中进行大规模的基因表达谱分析，可以让我们更好地了解牙周疾病的具体病理过程，为牙周疾病的预防开辟新的途径，并有助于牙周组织的再生。少

The periodontium functions as a single unit, even though each of its components has a distinct function, composition and connective tissue architecture. Thus it is important and significant to know molecular functions of each periodontal tissue cells using the genome-based research technology. In this study, we constructed the cell culture system depend on each periodontal tissue cell type, gingival epithelial, gingival fibroblastic, periodontal ligament derived, and osteoblatic cells, concerning to specific process of periodontal diseases, and analyzed a large scale of gene expression monitoring using bioinformatics technology. (1) Human gingival epithelial. cells and gingival fibroblasts were primary cultured from human normal gingival tissue, and a DNA microarray technique was used to analyze the different gene expressions. (2) Periodontal ligament derived cells were challenged with mechanical stress, periodontal pathogen LPS and Emdogain, and monitored those gene expression changes … More . (3) Osteoblasts were irradiated with low level diode laser and constructed subtractive gene library and analyzed genes enhanced transcription level, and further analyzed using DNA microarray technology. (4) Human mesenchymal bone marrow stem cells were induced to osteoblasts and monitored gene expression levels using GeneChip. (5) Human placenta trophoblasts were challenged by caffeine, and monitored gene expression levels using DNA inicroarray, further, rat placenta from caffeine feeding pregnant mouse were analyzed by RT-PCR. Effect of ageing on gene expression leve in submandibular glands of mouse was examined by DNA microarray. The reasons why we conducted these study were : periodontal diseases induced low birth infant; saliva components strongly supports health of periodontal tissues.A large scale of gene expression profiling conducted by our experimental models in each periodontal tissues cells, may let us to well understand the specific pathological process of periodontal diseases, to develop new approach of prevention of this disease, and to help the regeneration of periodontal tissues. Less