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by using DNA microarray

通过使用DNA微阵列

基本信息

批准号：
13470395
负责人：
ABIKO Yoshimitsu
金额：
$ 9.86万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The periodontium functions as a single unit, even though each of its components has a distinct function, composition and connective tissue architecture. Thus it is important and significant to know molecular functions of each periodontal tissue cells using the genome-based research technology. In this study, we constructed the cell culture system depend on each periodontal tissue cell type, gingival epithelial, gingival fibroblastic, periodontal ligament derived, and osteoblatic cells, concerning to specific process of periodontal diseases, and analyzed a large scale of gene expression monitoring using bioinformatics technology. (1) Human gingival epithelial. cells and gingival fibroblasts were primary cultured from human normal gingival tissue, and a DNA microarray technique was used to analyze the different gene expressions. (2) Periodontal ligament derived cells were challenged with mechanical stress, periodontal pathogen LPS and Emdogain, and monitored those gene expression changes … More . (3) Osteoblasts were irradiated with low level diode laser and constructed subtractive gene library and analyzed genes enhanced transcription level, and further analyzed using DNA microarray technology. (4) Human mesenchymal bone marrow stem cells were induced to osteoblasts and monitored gene expression levels using GeneChip. (5) Human placenta trophoblasts were challenged by caffeine, and monitored gene expression levels using DNA inicroarray, further, rat placenta from caffeine feeding pregnant mouse were analyzed by RT-PCR. Effect of ageing on gene expression leve in submandibular glands of mouse was examined by DNA microarray. The reasons why we conducted these study were : periodontal diseases induced low birth infant; saliva components strongly supports health of periodontal tissues.A large scale of gene expression profiling conducted by our experimental models in each periodontal tissues cells, may let us to well understand the specific pathological process of periodontal diseases, to develop new approach of prevention of this disease, and to help the regeneration of periodontal tissues. Less

牙周组织作为一个单一的单位发挥作用，即使它的每个组成部分都有不同的功能，组成和结缔组织结构。因此，利用基因组技术研究牙周组织细胞的分子功能具有重要意义。本研究针对牙周病的具体发病过程，构建了不同牙周组织细胞类型（牙龈上皮细胞、牙龈成纤维细胞、牙周韧带源性细胞、成骨细胞）的细胞培养体系，并利用生物信息学技术进行了大规模的基因表达监测分析。(1)人类牙龈上皮。原代培养人牙龈成纤维细胞和人牙龈成纤维细胞，应用基因芯片技术分析其基因表达差异。(2)牙周膜来源的细胞在机械应力、牙周病原菌LPS和Emdogain的刺激下，检测其基因表达的变化 ...更多信息 . (3)用低强度半导体激光照射成骨细胞，构建消减基因文库，分析基因的转录增强情况，并利用DNA微阵列技术进一步分析。(4)将人骨髓间充质干细胞诱导为成骨细胞，并使用基因芯片监测基因表达水平。(5)用咖啡因攻击人胎盘滋养层细胞，并用DNA微阵列监测基因表达水平，并对咖啡因喂养孕鼠的大鼠胎盘进行RT-PCR分析。应用基因芯片技术研究衰老对小鼠下颌下腺基因表达水平的影响。我们进行这些研究的原因是：牙周病引起的低出生儿;唾液成分对牙周组织的健康起着重要的作用，通过本实验模型对牙周组织各细胞进行大规模的基因表达谱分析，可以使我们更好地了解牙周病的具体病理过程，为牙周病的预防提供新的途径，并帮助牙周组织再生。少