Integrate research of genomics and proteomics of novel molecular targets for development of periodontal diseases cure

整合基因组学和蛋白质组学新分子靶点研究，开发牙周病治疗方法

• 批准号: 16209063
• 负责人: ABIKO Yoshimitsu
• 金额: $ 31.7万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209063/
• 关键词: Periodontal Research; Development of treatment; Genomics; Proteomics; Transcriptomics; GeneChip; Target molecules; プロテオミクス; MALDI TOF-MS; 歯周病病原菌; 歯周組織細胞; ゲノム; トランスクリプトーム; プロテオーム; バイオインフォーティクス; データベース; シグナル伝達系

Experimental research utilizing functional genomics technologies with DNA/protein database will serve to identification of genes and their expressions on the transcriptional level exemplify their utility for the understanding the life science. Several bacterial genome projects associated with oral infectious diseases are in progress. Human genome has been sequenced and assembled, making the accessible for genetic studies in near future. Recently, technological advances, such as subtractive gene cloning and DNA microarray, have been applied to discover of novel genes involved in complex metabolism. For example, since little is known regarding the molecules expressed by gingival epithelial cells that are involved in inflammation following the interaction with periodontal pathogens, to find transcriptional profiling of genes in human gingival epitherial cells in response to LPS, we examined many altered gene expressions using over 8,000 cDNA microarray (Incyte, GEM). To find transcriptional profiles of genes in submandibular gland by ageing, we examined mRNA levels of over 6,500 genes by nucleotide micreoarray (Affimetrix, GeneChip). Our laboratory involved in dental science projects using these technologies and genome database. By this Grant support, we plan to make the new database for oral diseases, which will be used easily by many researchers. The genome project and molecular biological approaches using the database will open the gate develop the dental science.

利用DNA/蛋白质数据库的功能基因组学技术的实验研究将有助于在转录水平上鉴定基因及其表达，举例说明它们在理解生命科学方面的实用性。一些与口腔传染病相关的细菌基因组计划正在进行中。人类基因组已被测序和组装，在不久的将来可用于基因研究。近年来，减法基因克隆和DNA微阵列等技术的进步已被用于发现参与复杂代谢的新基因。例如，由于对牙龈上皮细胞在与牙周病原体相互作用后参与炎症的分子表达知之甚少，为了找到人类牙龈上皮细胞对LPS反应的基因转录谱，我们使用超过8000个cDNA微阵列（Incyte， GEM）检测了许多改变的基因表达。为了发现衰老过程中颌下腺基因的转录谱，我们通过核苷酸微阵列（affmetrix, GeneChip）检测了6500多个基因的mRNA水平。我们的实验室使用这些技术和基因组数据库参与牙科科学项目。通过这项资助，我们计划建立一个新的口腔疾病数据库，方便许多研究人员使用。基因组计划和利用该数据库的分子生物学方法将为牙科科学的发展打开大门。

项目成果

Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of Porphyromonasgingivalis plasmid system.

使用牙龈卟啉单胞菌质粒系统测定赖氨酸特异性半胱氨酸蛋白酶（Lys-gingipain）的活性位点。

• 发表时间: 2008
• 期刊: Arch Oral Biol 53
• 作者: Ishida Y;Hu J;Sakai E;Kadowaki T;Yamamoto K;Tsukuba T;Kato Y;Nakayama K;Okamoto K.
• 通讯作者: Okamoto K.

Functional Analysis of the Thioredoxin Domain in Porphyromonas glnglvalis HBP35

绿色卟啉单胞菌 HBP35 硫氧还蛋白结构域的功能分析

• 发表时间: 2008
• 期刊: Bioscience, Biothechnology, and Biochemistry (印刷中)
• 作者: M.Wakatsuki;K.Teraguchi;E.Tomita;Yasuhiro Tajima;Etsuji Tomita;Mitsuo Wakatsuki;Akinobu Miyata;Dukka Bahadur;富田 悦次 (共著);富田 悦次(共著);富田 悦次(共著);富田 悦次(共著);置田 真生;置田 真生;田中 裕也;小谷 裕基;五味田 遵;前野 滝授;川崎 康博;平野 靖;Masao Okita;小谷 裕基;前野 滝授;置田 真生;村木 健介;Kensuke Muraki;川崎 康博;Fumihiko Ino;Fumihiko Ino;Fumihiko Ino;五味田 遵;永安 大輔;永安 大輔;川崎 康博;萩原 兼一;松井 学;Fumihiko Ino;松井 学;池田 孝利;池田 孝利;伊野 文彦;Fumihiko Ino;Yasuharu Mizutani;Fumihiko Ino;田中 裕也;合田 圭吾;Yasuhiro Kawasaki;Manabu Matsui;Takatoshi Ikeda;Fumihiko Ino;Fumihiko Ino;Kensuke Muraki;Kensuke Muraki;川崎 康博;伊野 文彦;FUmihiko Ino;Yasuharu Mizutani;Yasuharu Mizutani;村木 健介;Fumihiko Ino;合田 圭吾;Fumihiko Ino;Yasuharu Mizutani;水谷 泰治;置田 真生;伊野 文彦;松井 学;Manabu Matsui;松井 学;Yasuhiro Kawasaki;Manabu Matsui;Yasuhiro Kawasaki;Teruaki SHIROZA
• 通讯作者: Teruaki SHIROZA

Protein phosphorylation of 2-oxoglutarate oxidoreductase, beta subunit was induced by oxidative stress in Porphyromonas gingivalis

牙龈卟啉单胞菌氧化应激诱导 2-酮戊二酸氧化还原酶 β 亚基的蛋白质磷酸化

• 发表时间: 2007
• 期刊: Med Biol 印刷中
• 作者: Koi;S.;I. Tsukamoto;R. Inagawa;N. Kubo;R. Fujinami;R. Imaichi;M. Kato;金子 有子・國松 孝男・籠谷 泰行・中島 拓男;Yutaka IKAEDA;S.Okano
• 通讯作者: S.Okano

Proteomics-based analysis of a counter-oxidative stress system in Porphyromonas gingivalis.

基于蛋白质组学的牙龈卟啉单胞菌抗氧化应激系统分析。

• 发表时间: 2006
• 期刊: Proteomics 6
• 作者: Koi S;Fujinami R.;Kubo N.;Inagawa R.;Imaichi R.;Kato M.;Soichiro Okano
• 通讯作者: Soichiro Okano

Regulaton of BSP Gene Transcription by Amelogenins and Sheath Proteins

牙釉蛋白和鞘蛋白对 BSP 基因转录的调节

• 发表时间: 2007
• 作者: Yorimasa;Ogata;Yu;Nakajima;Li;Yang;Zhengyang;Li;Zhitao;Wang;Xinyue;Li;Masaru;Mezawa;Shouta;Araki;Youhei;Nakayame;Shinichiro;Oida;Makoto;Fukae
• 通讯作者: Fukae