Study of effect of host nicotine metabolism on progress of periodontitis in smokers

（2001财政年度）通过测定唾液中尼古丁代谢物可替宁的浓度，比较了吸烟者和非吸烟者牙周病病情与唾液潜血反应结果的关系。结果，吸烟组隐血阴性患者较多，但牙周炎有进展，且唾液中可替宁浓度较高组牙周炎有向严重程度发展的趋势。在牙周炎进展组，唾液潜血反应试验与唾液中可替宁浓度呈显著负相关，唾液潜血量随唾液中可替宁浓度升高而降低。（2002财政年度）作为体液中可替宁和尼古丁的定量方法，旨在发展高效液相色谱法（HPLC）。通过不同洗脱条件下不同色谱柱和流动相的变化，检索了烟碱和可替宁的超灵敏检测条件，建立了烟碱和可替宁在94 ~ 2350ng/ml和122 ~ 3050ng/ml范围内的直线定量方法。然而，由于与可替宁峰重叠的物质混合在一起，这种检查对唾液成分并不好。当将样品改为血清时，可以确定不存在妨碍可替宁和尼古丁含量的峰，可以很好地进行分析。（2003财政年度）采用高效液相色谱法对某卫生院体检者413人进行了牙周健康调查评价结果与吸烟量、血液中可替宁量的各关系进行了检测。根据问卷投票的吸烟量和HPLC定量测定的可替宁量与牙周炎的进展有显著的相关性。同时，在问卷投票显示吸烟量较多但血液中可替宁含量不高的受试者中，牙周炎有进展的趋势。也就是说，在尼古丁代谢较低的人身上，毒性持续影响，表明牙周炎进展的可能性。少

(The 2001 fiscal year)The relationship between disease state of periodontal disease and occult blood reaction test result in the saliva in the smoker and nonsmoker was compared and was examined by the measurement of the saliva concentration of the cotinine which is a metabolite of the nicotine. As the result, there were many occult blood negative subjects in the smoking group, though periodontitis progresses, and the periodontitis tended to make it into the seriousness in the group of which the cotinine concentration in the saliva was higher. In the group in which the periodontitis progressed, there was the significant inverse correlation between saliva occult blood reaction test and cotinine concentration in the saliva, and the saliva occult blood quantity lowered with the rise in the cotinine concentration in the saliva.(The 2002 fiscal year)As a quantitative method of cotinine and nicotine in body fluid, it aimed at the development of the method using high performance liquid chiroma … More tography(HPLC). As a result of retrieving the condition for supersensitively detecting nicotine and cotinine by the change of column and mobile phase variously using various elution conditions, the method for obtaining the rectilinear quantitativity for nicotine and cotinine was able to be developed in 94〜2350ng/ml and in 122〜3050ng/ml, respectively. However, this inspection was not good for the saliva component, because the material which overlapped with the peak of the cotinine intermingled. It was able to be confirmed that there was no peak which obstructs quantity of cotinine and nicotine and that the analysis can be carried out well, when the sample was changed to be the serum.(The 2003 fiscal year)Medical examinees of 413 persons of the some health care center were examined on each relation of assessment result of periodontal health survey and smoking quantity according to the questionnaire vote and cotinine quantity in the blood by HPLC. The significant correlation as the result at smoking quantity according to the questionnaire vote and cotinine quantity determined quantitatively by HPLC and progress of periodontitis was recognized. In the meantime, in the subjects whose smoking quantity was abundant according to the questionnaire vote but cotinine quantity in the blood was not high, periodontitis tended to advance. That is, in the person whoes metabolism of the nicotine is lower, it was that the toxicity continuously affects, and the possibility that the progress of periodontitis advances was indicated. Less