Study of effect of oral microflora on oral and general health

在这项研究中，我们检查了口腔菌群与牙周炎和恶臭的关系，并进一步分析了与住院老年人发烧天数和肺炎的关系。为了确定口腔菌群的组成，我们从唾液或舌苔中提取细菌的染色体DNA，并以提取的细菌DNA为模板进行末端限制性片段长度多态性分析。结果表明，唾液细菌的T-RFLP模式与牙周炎和恶臭的患病率密切相关。此外，机构老年人舌苔T-RFLP模式也与发热天数和肺炎相关。

In this study, oral microflora was examined in relationship to periodontitis and malodor, and was further analyzed with febrile days and pneumonia in institutionalized elderly. To characterize the composition of oral microflora, chromosomal DNA was extracted from bacteria in saliva or tongue coat and Terminal-Restriction Fragment Length Polymorphism analysis was carried out using the extracted bacterial DNA as template. The results indicated that T-RFLP pattern of salivary bacteria was closely associated with the prevalence of periodontitis and malodor. In addition, T-RFLP pattern in tongue coat was also associated with febrile days and pneumonia in institutionalized elderly.