Study of effect of oral microflora on oral and general health

口腔微生物群对口腔和全身健康影响的研究

• 批准号: 19390541
• 负责人: YAMASHITA Yoshihisa
• 金额: $ 12.06万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390541/
• 关键词: 口腔フローラ; T-RFLP; 口腔疾患; 全身の健康; 16S rRNA遺伝子; 網羅的解析; 口腔の健康; 口腔細菌叢; 唾液検査

In this study, oral microflora was examined in relationship to periodontitis and malodor, and was further analyzed with febrile days and pneumonia in institutionalized elderly. To characterize the composition of oral microflora, chromosomal DNA was extracted from bacteria in saliva or tongue coat and Terminal-Restriction Fragment Length Polymorphism analysis was carried out using the extracted bacterial DNA as template. The results indicated that T-RFLP pattern of salivary bacteria was closely associated with the prevalence of periodontitis and malodor. In addition, T-RFLP pattern in tongue coat was also associated with febrile days and pneumonia in institutionalized elderly.

本研究探讨机构老人口腔菌群与牙周炎及口臭的关系，并进一步分析其与发热天数及肺炎的关系。为了表征口腔微生物菌群的组成，从唾液或舌苔中的细菌中提取染色体DNA，并以提取的细菌DNA为模板进行末端限制性片段长度多态性分析。结果表明，唾液细菌T-RFLP多态性与牙周炎和口臭的患病率密切相关。此外，舌苔T-RFLP模式也与机构老年人的发热天数和肺炎有关。

项目成果

The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status

• DOI: 10.1038/ismej.2008.91
• 发表时间: 2009-01-01
• 期刊: ISME JOURNAL
• 影响因子: 11
• 作者: Takeshita, Toru;Nakano, Yoshio;Yamashita, Yoshihisa
• 通讯作者: Yamashita, Yoshihisa

The change of microbial populations during plaque development.

斑块形成过程中微生物种群的变化。

• 发表时间: 2008
• 作者: Masaki Yasui;et al.
• 通讯作者: et al.

Improved accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standard definition

• DOI: 10.1111/j.1399-302x.2007.00384.x
• 发表时间: 2007-12-01
• 期刊: ORAL MICROBIOLOGY AND IMMUNOLOGY
• 作者: Takeshita, T.;Nakano, Y.;Yamashita, Y.
• 通讯作者: Yamashita, Y.

Relationship between oral malordor and the global composition of indigenous bacterial populations in salaiva Appl Environ Microbiol.

口腔恶臭与唾液中本土细菌种群整体组成之间的关系 Appl Environ Microbiol。

• 发表时间: 2010
• 期刊: (in press)
• 作者: Takeshita T;Suzuki N;Nakano Y;Shimazaki Y;Yoneda M;Hirofqi T;Yamashita Y
• 通讯作者: Yamashita Y

Improveded accuracy in terminal restriction fragment length polymorphism phylogenetic analysis using a novel internal size standarddefinition.

使用新的内部大小标准定义提高了末端限制性片段长度多态性系统发育分析的准确性。

• 发表时间: 2007
• 期刊: Oral Microbiol Immunol 22(6)
• 作者: Takeshita T;Nakano Y;Yamashita Y
• 通讯作者: Yamashita Y