Establish of clinical evaluation system for measurement of carcinogen (acetaldehyde) derived from oral bacterium

(The为了建立灵敏的口腔乙醛生成能力测定方法，我们尝试了GC/MS法测定乙醛的羟胺衍生物，与传统的HPLC法相比，该方法具有较高的灵敏度。(The 2005财政年度），当将新开发的使用HPLC的CNET盒应用于九州大学牙科系的10名学生时，我们可以获得与上述GC/MS方法相同的在用乙醇或葡萄糖漱口后测量呼吸中浓度的灵敏度。开发了16 S rRNA基因的末端限制性片段长度多态性（TRFLP），以鉴定乙醛身高学生特有的微生物区系模式。通过与核糖体数据库计划II（RDPII）数据库中的50000种细菌的遗传信息进行比较，能够在短时间内鉴定与特定植物群相关的细菌种类。(The 2006财政年度）当九州大学牙科系的70名学生接受检查时，用乙醇漱口后呼气中乙醛的浓度分布在0.4至2.2 μg/ml之间。聚类分析将TRFLP型分为3类。没有与乙醛产生相关的特定簇。然而，一个峰被认为与乙醛的产生密切相关。当与RDBPII数据库比较时，分配给该峰的细菌种属为放线菌属。

(The 2004 fiscal year) To establish the sensitive method to measure the ability to produce acetaldehyde in mouth, we tried the method measuring its hydroxylamine derivative by GC/MS. This method showed higher sensitivity compared with conventional measurement by HPLC.(The 2005 fiscal year) We could obtain sensitivity in measuring a concentration in the breath after mouth rinse with the ethanol or glucose equal to that in the method by GC/MS described above, when the newly developed CNET cartridge using HPLC was applied to ten students of Kyushu University Faculty of Dentistry. Terminal Restriction Fragment Length Polymorphism (TRFLP) of the 16S rRNA gene was developed to identify the pattern of microflora specific to the acetaldehyde height student. It became possible that the bacterial species related to specific flora was able to be identified in a short time by comparison with genetic information on 50000 kinds of bacteria in the database of Ribosomal Database Project II (RDPII).(The 2006 fiscal year) The concentration of acetaldehyde in breath distributed from 0.4 to 2.2 μg/ml after mouth rinse with ethanol, when seventy students of Kyushu University Faculty of Dentistry were examined. TRFLP patterns were classified into three groups by cluster analysis. There was no specific cluster related to acetaldehyde production. However, one peak was recognized to be closely related to acetaldehyde production. The bacterial species assigned to this peak was Actinomyces species when compared with the database of RDBPII.