Establish of clinical evaluation system for measurement of carcinogen (acetaldehyde) derived from oral bacterium

(2004财年)为了建立灵敏的测定口腔中乙醛生成能力的方法，我们尝试了用GC/MS测定其羟胺衍生物的方法。该方法比传统的高效液相色谱测定方法具有更高的灵敏度。(2005财年)用新研制的高效液相色谱CNET色谱柱对九州大学牙科学院的10名学生进行检测，可以获得与上述方法相同的灵敏度。建立了16S rRNA基因的末端限制性片段长度多态性(TRFLP)方法，以确定乙醛高中生特有的微生物区系模式。通过与核糖体数据库项目II数据库中50000种细菌的遗传信息进行比较，可以在短时间内鉴定出与特定菌群相关的细菌种类。(2006年)九州大学口腔医学院70名学生用乙醇漱口后，呼气中乙醛的浓度分布在0.4~2.2μg/ml之间。通过聚类分析，将TRFLP分成3组。没有与乙醛产生相关的特异簇。然而，有一个峰被认为与乙醛的产生密切相关。与RDBPII数据库比较，归属于该峰的细菌种类为放线菌。

(The 2004 fiscal year) To establish the sensitive method to measure the ability to produce acetaldehyde in mouth, we tried the method measuring its hydroxylamine derivative by GC/MS. This method showed higher sensitivity compared with conventional measurement by HPLC.(The 2005 fiscal year) We could obtain sensitivity in measuring a concentration in the breath after mouth rinse with the ethanol or glucose equal to that in the method by GC/MS described above, when the newly developed CNET cartridge using HPLC was applied to ten students of Kyushu University Faculty of Dentistry. Terminal Restriction Fragment Length Polymorphism (TRFLP) of the 16S rRNA gene was developed to identify the pattern of microflora specific to the acetaldehyde height student. It became possible that the bacterial species related to specific flora was able to be identified in a short time by comparison with genetic information on 50000 kinds of bacteria in the database of Ribosomal Database Project II (RDPII).(The 2006 fiscal year) The concentration of acetaldehyde in breath distributed from 0.4 to 2.2 μg/ml after mouth rinse with ethanol, when seventy students of Kyushu University Faculty of Dentistry were examined. TRFLP patterns were classified into three groups by cluster analysis. There was no specific cluster related to acetaldehyde production. However, one peak was recognized to be closely related to acetaldehyde production. The bacterial species assigned to this peak was Actinomyces species when compared with the database of RDBPII.