Establish of clinical evaluation system for measurement of carcinogen (acetaldehyde) derived from oral bacterium

（2004会计年度）为建立测定乙醛在口腔中产生能力的灵敏方法，我们尝试了用GC/MS测定其羟胺衍生物的方法。与常规HPLC法相比，该方法具有较高的灵敏度。（2005财政年度）对九州大学牙科学院的10名学生使用新开发的高效液相色谱CNET药盒，用与上述方法相同的乙醇或葡萄糖测量漱口后呼气中的浓度，可以获得与上述方法相同的灵敏度。开发了16S rRNA基因的末端限制性片段长度多态性（TRFLP），以鉴定乙醛高度学生特有的微生物群模式。通过比较核糖体数据库ⅱ（RDPII）数据库中50000种细菌的遗传信息，在短时间内鉴定出与特定菌群相关的细菌种类成为可能。（2006会计年度）对九州大学牙科学院70名学生进行了酒精漱口后呼气中乙醛浓度分布在0.4 ~ 2.2 μg/ml之间。聚类分析将TRFLP模式分为三类。没有与乙醛生产相关的特定簇。然而，有一个峰值被认为与乙醛的产生密切相关。与RDBPII数据库比较，归属于该峰的细菌种为放线菌种。

(The 2004 fiscal year) To establish the sensitive method to measure the ability to produce acetaldehyde in mouth, we tried the method measuring its hydroxylamine derivative by GC/MS. This method showed higher sensitivity compared with conventional measurement by HPLC.(The 2005 fiscal year) We could obtain sensitivity in measuring a concentration in the breath after mouth rinse with the ethanol or glucose equal to that in the method by GC/MS described above, when the newly developed CNET cartridge using HPLC was applied to ten students of Kyushu University Faculty of Dentistry. Terminal Restriction Fragment Length Polymorphism (TRFLP) of the 16S rRNA gene was developed to identify the pattern of microflora specific to the acetaldehyde height student. It became possible that the bacterial species related to specific flora was able to be identified in a short time by comparison with genetic information on 50000 kinds of bacteria in the database of Ribosomal Database Project II (RDPII).(The 2006 fiscal year) The concentration of acetaldehyde in breath distributed from 0.4 to 2.2 μg/ml after mouth rinse with ethanol, when seventy students of Kyushu University Faculty of Dentistry were examined. TRFLP patterns were classified into three groups by cluster analysis. There was no specific cluster related to acetaldehyde production. However, one peak was recognized to be closely related to acetaldehyde production. The bacterial species assigned to this peak was Actinomyces species when compared with the database of RDBPII.