Biophysical Chemistry on photobrhodopsin, a sensor for a negative taxis of Halobacterium

光视紫红质的生物物理化学，一种盐杆菌负趋向性传感器

• 批准号: 13470475
• 负责人: KAMO Naoki
• 金额: $ 8.45万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470475/
• 关键词: photocycle; halobacterial transducer; SVD; archaeal rhodopsin; flash photolysis; bacteriorhodopsin; color regulation; retinal protein; 高度好塩菌; バクテリオロドプシ; フォボロドプシン; Natronobacterium pharaonis; 吸収波長; 走光性; 固体NMR; 膜タンパク安定性; Halobacterium halobi

pharaonis phoborhodopsin (ppR) is a sensor for negative phototaxis of Natronobacterium pharaonis. Illumination excites ppR, which relaxed to the original ground state of ppR. This linear and cyclic photochemistry is called photocycle. Within membranes, ppR forms a complex with a transducer protein, pHtrII, and this complex transmits photo-signals to cytoplasm. This research clarified as follows(1) Discovery of a new photo-intermediate after high-pressure experiments and SVD analysis.(2) Assignment of an amino acid residue from which proton is released during photocycling.(3) Determination of the stoichiometry of the complex(4) Assignment of essential amino acid residues for the formation of the complex.(5) One hundred-fold alternation of KD values of the complex during photocycling.(6) Importance of helix-helix interaction for the color tuning.

pharaonis phoborhodopsin（ppR）是一种负趋光性感受器。光照激发ppR，其弛豫到ppR的原始基态。这种线性和循环的光化学过程称为光循环。在膜内，ppR与转导蛋白pHtrII形成复合物，并且该复合物将光信号传输到细胞质。通过高压实验和SVD分析，发现了一种新的光致中间体。(2)在光循环过程中释放质子的氨基酸残基的归属。(3)配合物化学计量的确定（4）配合物形成所必需的氨基酸残基的归属。(5)在光循环过程中，复合物的KD值变化了100倍。(6)螺旋-螺旋相互作用对颜色调节的重要性。

项目成果

Masayuki Iwamoto et al.: "Role of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II)."Biophys.J.. 83. 1130-1135 (2002)

Masayuki Iwamoto 等人：“Asp193 在 pharaonis phoborhodopsin（感觉视紫红质 II）发色团-蛋白质相互作用中的作用。”Biophys.J. 83. 1130-1135 (2002)

Y.Sudo, M.Yamabi, M.Iwamoto, K.Shimono, N.Kamo: "Interaction of Natronobacterium pharaonis phoborhodopsin (sensory rhodopsin II) with its cognate transducer probed by increase in the thermal stability"Photochem.Photobiol.. 78. 511-516 (2003)

Y.Sudo、M.Yamabi、M.Iwamoto、K.Shimono、N.Kamo：“通过增加热稳定性来探测法老嗜碱杆菌 phoborhodopsin（感觉视紫红质 II）与其同源传感器的相互作用”Photochem.Photobiol.. 78。

Y.Furutani, Y.Sudo, N.Kamo, H.Kandori: "FTIR spectroscopy of the complex between pharaonis phoborhodopsin and its transducer protein"Biochemistry. 42. 4837-4842 (2003)

Y.Furutani、Y.Sudo、N.Kamo、H.Kandori：“法老荧光紫红质及其转导蛋白之间复合物的 FTIR 光谱”生物化学。

Yuki Sudo: "Association between a photo-intermediate of D75N of pharaonis phoborhodopsin and its cognate transducer"J. Photochem. Photobiol.. 67. 171-176 (2002)

Yuki Sudo：“法老荧光紫红质 D75N 的光中间体与其同源传感器之间的关联”J。

Yuki Sudo et al.: "Interaction of Natronobacterium pharaonis phoborhodopsin (sensory rhodopsin II) with its cognate transducer probed by increase in the thermal stability."Photochem.Photobiol.. 78. 511-516 (2003)

Yuki Sudo 等人：“通过增加热稳定性来探测法老嗜碱杆菌磷紫红质（感觉视紫红质 II）与其同源传感器的相互作用。”Photochem.Photobiol.. 78. 511-516 (2003)