Physical and/or Chemical Methods for the Removal of Virus from Transfusion Blood

从输血中去除病毒的物理和/或化学方法

• 批准号: 12794016
• 负责人: KAMO Naoki
• 金额: $ 22.08万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12794016/
• 关键词: parvovirus; UV-irradiation; UVC; Heat treatment of virus; S; D treatment; Hemoglobin; CFU; DNA damage; ヒトパルボウイルスB19; カテキン; メチレンブルー; 活性酸素; ピリミジンダイマー; 6-4付加体; 血液凝固第VIII因子; 一重項酸素; B19; 血液凝固第8因子

Blood for transfusion is subject to rigorous inspections for not contaminating virus. However, there are some inevitable possibilities for the contamination because of 1) the necessity of time to produce antibodies against the infected virus (window period) and 2) the existence of unknown virus. Therefore the aim of this research is to develop method of the removal or inactivation of virus in the transfusion blood.1) We concentrated on parvovirus B19, one of non-enveloped virus which is resistant to the solvent/detergent or heat treatment. In addition, infection of this virus sometimes gives sever illness. First of all, we developed the method testing the infectivity using CFU-E formation. Irradiation of UV-C (750 Jm^<-2>) deceased the infectivity by a factor of 3. The co-presence of rutin or catechins prevents the denaturation of factor VIII without any decrease in the effect against the virus.2) The UV-C treatment was found to be very useful for the inactivation of virus containing hemoglobin solutions that will be applied to emergency patients who need a lot of blood.3) We observed a good correlation between the inactivation of virus and the amounts of DNA damage.

用于输血的血液必须经过严格的检查，以确保不污染病毒。然而，污染存在一些不可避免的可能性，因为1）需要时间来产生针对感染病毒的抗体（窗口期）和2）未知病毒的存在。因此，本研究的目的是开发出一种有效的去除或灭活输血中病毒的方法。1）我们主要研究细小病毒B19，它是一种无包膜病毒，对溶剂/去污剂和热处理都有抵抗力。此外，这种病毒的感染有时会导致严重的疾病。首先，我们开发了使用CFU-E形成测试感染性的方法。UV-C（750 Jm^）照射<-2>使感染性降低3倍。芦丁或儿茶素的共同存在防止了因子VIII的变性，而不会降低对病毒的作用。2）发现UV-C处理对于将应用于需要大量血液的急诊患者的含有血红蛋白溶液的病毒的灭活非常有用。3）我们观察到病毒灭活与DNA损伤量之间的良好相关性。

项目成果

J.Hirayama, H.Abe, N.Kamo, K.Ikebuchi, H.Ikeda.: "Comparison of the effects of different antiviral treatments on the antioxidant systems of stroma-free hemoglobin"Photochem Photobio1. 74(3). 461-464 (2001)

J.Hirayama、H.Abe、N.Kamo、K.Ikebuchi、H.Ikeda.：“不同抗病毒治疗对无基质血红蛋白抗氧化系统的影响比较”Photochem Photobio1。

Y.Naito et al.: "Virus removal from hemoglobin solution using Planova Membrane"J Artif Organs. 5. 141-145 (2002)

Y.Naito 等人：“使用 Planova 膜从血红蛋白溶液中去除病毒”J Artif Organs。

Y.Yamada-Ohnishi et al.: "Cell-associated virus infectivity of primary HIV-1 isolate can be eliminated by a filtration/methylene blue photoinactivation system"Transfusion. 40(12). 1542-1543 (2000)

Y.Yamada-Ohnishi 等人：“可以通过过滤/亚甲基蓝光灭活系统消除初级 HIV-1 分离株的细胞相关病毒感染性”输血。

Y.Yamada-Ohnishi, T.Owada, H.Abe, J.Hirayama, H.Ikeda, K.Ikebuchi.: "Cell-associated virus infectivity of primary HIV-1 isolate can be eliminated by a filtration/methylene blue photoinactivation system"Transfusion. 40(12). 1542-1543 (2000)

Y.Yamada-Ohnishi、T.Owada、H.Abe、J.Hirayama、H.Ikeda、K.Ikebuchi.：“可以通过过滤/亚甲蓝光灭活系统消除初级 HIV-1 分离株的细胞相关病毒感染性

J.Hirayama et al.: "Comparison of the effects of different antiviral treatment on the antioxidant systems of stroma-free hemoglobin"Photochem. Photobiol.. 74. 461-464 (2001)

J.Hirayama 等人：“不同抗病毒治疗对无基质血红蛋白抗氧化系统的影响比较”Photochem。