Comparison of Photochemistry for various retinal-proteins : Consideration for relationship between their structure and function

各种视网膜蛋白光化学的比较：考虑它们的结构和功能之间的关系

• 批准号: 04302065
• 负责人: KAMO Naoki
• 金额: $ 5.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04302065/
• 关键词: Rhodopsin; Bacteriorhodopsin; Halorhodopsin; Phoborhodopsin; Paramecium; Chlamydomonas; Halobacteria; Photochemistry; ゾーリムシ; ホボロドプシン; ゾーリ虫

(1) Photochemistry of pharaonis phoborhodopsin (ppR) was made clear and the structure of binding pocket of retinal was estimated.(2) Mutant retinal proteins were obtained both from naturallyhabitant halobacteria and by artificial DNA.Characterization gave clues on amino acids essential for the function.(3) Octopus rhodopsin was well characterized.(4) Structural changes of bacteriorhodopsin, Octopus rhodopsin were investigated by FTIR and by Raman spectroscopy.(5) Crystallization of Squid rhodopsin and bacteriorhodopsin were tried and interesting findings were obtained.(6) Interference of phototaxis of Chlamydomonas by photosynthesis was first showed. Rhodopsin-like proteins and mechanism of photo-sensory were clarified.(7) Molecular dynamic theory of retinal isomerization and analysis of vibration mode of retinal were developed.

(1)明确了幽门螺视紫红质(PPR)的光化学，并估算了视网膜结合袋的结构。(2)从自然栖息的盐杆菌和人工DNA中都获得了突变的视网膜蛋白。其特性为揭示其功能所必需的氨基酸提供了线索。(3)章鱼视紫红质得到了很好的表征。(4)用傅里叶变换红外光谱和拉曼光谱研究了细菌视紫红质和章鱼视紫红质的结构变化。(5)尝试了鱿鱼视紫红质和细菌视紫红质的结晶，并得到了有趣的发现。(6)首次发现了光合作用对衣藻光合作用的干扰。提出了视网膜异构化的分子动力学理论和视网膜振动模式分析。

项目成果

Kouyama,T.: "Bacteriorhodopsin,a bilogical proton pump working in the ligh." RIKEN Review. 1. 5-6 (1993)

Kouyama，T.：“细菌视紫红质，一种在光中工作的生物质子泵。”

Mizutani,Y.et al.: "Resonance Raman spectra of the photointermediates in phytochrome phototransformation" Biochemistry. 33. 153-158 (1994)

Mizutani,Y.et al.：“光敏色素光转化中光中间体的共振拉曼光谱”生物化学。

Takahashi,T.et al.: "Photosynthesis modulates the sign of phototaxis of wild-type Chlamydomonas reinhardtii" FEBS Lett.336. 516-520 (1993)

Takahashi,T.et al.：“光合作用调节野生型莱茵衣藻的趋光性迹象”FEBS Lett.336。

Kandori,H.,et al.: "Comparative study of primary photochemical events of two retinal proteins,bacteriorhodpsin and halorhodopsin" Chem.Phys.Lett.211. 385-391 (1993)

Kandori,H.,et al.：“两种视网膜蛋白细菌视紫红质和盐视紫红质初级光化学事件的比较研究”Chem.Phys.Lett.211。

Y.Imamoto,: "Chromophore Configuration of pharaonis phoborhodopsin and its isomerization on photon absorption" Biochemistry. 31. 2523-2528 (1992)

Y.Imamoto，：“法老荧光紫红质的发色团构型及其对光子吸收的异构化”生物化学。