PHYSICO-CHEMICAL STUDY ON PHARAONIS PHOBORHODOPSIN USING ITS RECOMBINANT PROTEIN
PHYSICO-CHEMICAL STUDY ON PHARAONIS PHOBORHODOPSIN USING ITS RECOMBINANT PROTEIN
批准号:
10470473
负责人:
KAMO Naoki
金额:
$6.78万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999
中文摘要
1.成功构建了PPR的表达系统。M-中间体的衰变非常缓慢,但添加100 mM叠氮可使其加速300倍。提出了慢M衰变的可能原因,并在实验上得到了证实。考虑了一种可能的颜色调谐机制。对PPR进行了分子动力学计算。
英文摘要
1. We eastblishied an expression system of pharaonis phoborhodopsin (ppR).2. A decay of a M-intermediate is very slow, but was accelerated 300-fold by an addition of 100 mM azide.3. A possible reason of the slow M-decay was proposed and was confirmed experimentally.4. A possible mechanism of color tuning was considered.5. Molecular dynamics calculation of ppR was done.
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T.Baba他5名: "Formation and characterization of planar lipid bilayer membranes from synthetic phytanyl-chained glycolipids"Biochim.Biopys.Acta. 1421. 91-102 (1999)
T. Baba 和其他 5 人:“合成植烷链糖脂的平面脂质双层膜的形成和表征”Biochim.Biopys.Acta。1421. 91-102 (1999)
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M.Iwamoto他4名: "Proton uptake and release of pharaonis Phoraonis Phoborhodopsin detected by a photoelectrochemical cell"J.Phys.Chem.Part B. 103. 10311-10315 (1999)
M. Iwamoto 和其他 4 人:“光电化学电池检测到的法老荧光紫红质的质子摄取和释放”J.Phys.Chem.Part B. 103. 10311-10315 (1999)
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H.Abe他5名: "Conjugation of dipeptide to fluorescent dyes enhances its affinity for a dipeptide transporter (PEPT1) in human intestinal Caco-2 cells"Bioconjugate Chem.. 10. 24-31 (1999)
H.Abe 和其他 5 人:“二肽与荧光染料的结合增强了其对人肠道 Caco-2 细胞中二肽转运蛋白 (PEPT1) 的亲和力”Bioconjugate Chem.. 10. 24-31 (1999)
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M. Komatubara et al.: "Cimetidine enhances the antiproliferative effect of 5-fluorouracil on colon carcinome SW620."Anticancer Res.. 19(2A). 1153-1157 (1999)
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共 36 条
photochemistry of sensory rhodopsin
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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Elucidation of Cl--pumping mechanism and significance of trimer formation of halorhodopsin
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Signal transduction via membrane protein complex : photo-signal transduction of Halobacterium salinarum
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财政年份:2004
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Social-philosophical Research of the Urgent problem of Japan in 21st century
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批准号:13410004
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.09万
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财政年份:2001
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负责人:KAMO Naoki
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依托单位:
Biophysical Chemistry on photobrhodopsin, a sensor for a negative taxis of Halobacterium
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批准号:13470475
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.45万
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财政年份:2001
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负责人:KAMO Naoki
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Physical and/or Chemical Methods for the Removal of Virus from Transfusion Blood
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批准号:12794016
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项目类别:Grant-in-Aid for University and Society Collaboration
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资助金额:$22.08万
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财政年份:2000
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负责人:KAMO Naoki
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依托单位:
Physicochemical Studies on multi-drug Efflux Transporters of Bacteria
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批准号:08457600
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$4.74万
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财政年份:1996
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负责人:KAMO Naoki
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依托单位:
Drug Delivery System Utilizing Transporters That Recognize Substrates of Relatively Large Molecular Sizes
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批准号:07557142
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$4.74万
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财政年份:1995
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负责人:KAMO Naoki
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依托单位:
Study on membrane transport of halobacterial cells used as model cells for the transport investigation of pharmaceutical agents
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批准号:04452302
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.35万
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财政年份:1992
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负责人:KAMO Naoki
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依托单位:
Comparison of Photochemistry for various retinal-proteins : Consideration for relationship between their structure and function
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批准号:04302065
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项目类别:Grant-in-Aid for Co-operative Research (A)
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资助金额:$5.06万
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财政年份:1992
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负责人:KAMO Naoki
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依托单位:
Development of Fluorescent Microscope Having Multi-Excitation for Simultaneous Measurements of Several Dyes in Order to Investigate Cell Functions
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批准号:02557087
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$6.85万
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财政年份:1990
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负责人:KAMO Naoki
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依托单位:
Physicochemical Study on Intra-callular Information Processing
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批准号:01460242
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.97万
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财政年份:1989
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负责人:KAMO Naoki
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依托单位:
Physicochemical Basis of Information Processing in Ameboid Cells in Relation to Perception of Lower Organisms
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批准号:62460214
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.1万
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财政年份:1987
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负责人:KAMO Naoki
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依托单位:
Studies on Retinoid Proteins in Membranes of Halobacterium halobium
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批准号:60571012
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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负责人:KAMO Naoki
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依托单位:
海外基金