Synthesis of new functionalized nucleic acids by the pentaerythritol linker.

通过季戊四醇接头合成新的功能化核酸。

基本信息

  • 批准号:
    13470481
  • 负责人:
  • 金额:
    $ 5.95万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

The 3'-3'-linked oligodeoxynucleotides (ODNs) by pentaerythritol with an intercalator at the junction point is that can form the parallel triplexes with the duplex target by Hoogsteen hydrogen bonds were synthesized. Stability of the triplexes with the target duplexes was studied by thermal denaturation. The 3'-3'-linked ODNs with the anthraquinonyl group enhanced the thermal stability of the triplexes when compared with those without the intercalator and the unmodified nonamer. It was found that the ODNs carrying the anthraquinonyl groups can form thermally stable triplexes by skipping two or three extra base pairs between two binding domains of the target duplexes. The 3'-3'-linked ODN with the anthraquinonyl group at the junction point inhibited the cleavage by the enzyme Hind III more effectively.We also synthesized the 3'-3'-linked TFOs that can form the antiparallel triplexes with the duplex DNA target by reverse Hoogsteen hydrogen bonds. The alternate-strand triplexes were significantly stabilized by linking the TFO fragments with the pentaerythritol linker. And, unlike the alternate-strand triplexes composed of the pyrimidine motif, the terminal ammonium ion of the aminobutyl-linker and the intercalator of the TFOs did not contribute to the stability of the alternate-strand triplex comprised of the purine motif.
以季戊四醇为原料,在连接点加入插层剂,合成了3′-3′连接的寡脱氧核苷酸(ODNs),通过Hoogsteen氢键与双工靶形成平行的三络合物。用热变性法研究了三络合物与目标络合物的稳定性。3′-3′连接蒽醌基的odn与不含插入物和未修饰的氨基化合物相比,热稳定性得到了提高。研究发现,携带蒽醌基的odn可以通过在目标双链的两个结合域之间跳过两个或三个额外的碱基对而形成热稳定的三链。3‘-3’连接的ODN在接点处含有蒽醌基,能更有效地抑制Hind III酶的裂解作用。我们还通过反向Hoogsteen氢键合成了3‘-3’链的tfo,它可以与双链DNA靶标形成反平行的三联体。将TFO片段与季戊四醇连接后,交替链三聚体得到了明显的稳定。而且,与嘧啶基序组成的交替链三联体不同,氨基丁基连接体的末端铵离子和TFOs的插层剂对嘌呤基序组成的交替链三联体的稳定性没有贡献。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ueno, Y., et al.: "Synthesis of the 3'-3'-linked oligonucleotides branched by the pentaerythritol linker and the thermal stabilities of the triplexes with the single stranded DNA or RNA"Bioconjugate Chem.. (in press).
Ueno, Y., 等人:“由季戊四醇接头分支的 3-3-连接寡核苷酸的合成以及具有单链 DNA 或 RNA 的三链体的热稳定性”Bioconjugate Chem..(出版中)。
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    0
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Hoshika, S. et al.: "Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides using a purine motif"Bioconjugate Chem.. (in press). (2002)
Hoshika, S. 等人:“使用嘌呤基序通过 3-3 连接的寡脱氧核苷酸形成交替链三螺旋”Bioconjugate Chem..(出版中)。
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    0
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Ueno, Y., Mikawa, M., Hoshika, S., Matsuda, A.: "Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides with the anthraquinonyl group at the junction point"Bioconjugate Chem.. 12. 635-642 (2001)
Ueno, Y.、Mikawa, M.、Hoshika, S.、Matsuda, A.:“3-3-连接的寡脱氧核苷酸与连接点上的蒽醌基团形成交替链三螺旋”Bioconjugate Chem。
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    0
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Ueno, Y. Shibata, A., Matsuda, A, Kitade, Y.: "Synthesis of the 3'-3'-linked oligonucleotides branched by the pentaerythritol linker and the thermal stabilities of the triplexes with the single stranded DNA or RNA"Bioconjugate Chem.. in press.
Ueno, Y. Shibata, A.、Matsuda, A、Kitade, Y.:“季戊四醇接头分支的 3-3 连接寡核苷酸的合成以及单链 DNA 或 RNA 三链体的热稳定性”
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    0
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Ueno, Y., et al.: "Alternate-stand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides with the anthraquinonyl group at the junction point"Nucleic Acids Res.. Suppl.1. 11-12 (2001)
Ueno, Y., et al.:“3-3-连接的寡脱氧核苷酸与连接点处的蒽醌基形成交替链三螺旋”Nucleic Acids Res.. Suppl.1。
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MATSUDA Akira其他文献

Contribution of IL-33 to allergic airway inflammation in mice sensitized subcutaneously and challenged intranasally with cysteine protease allergen.
IL-33 对皮下致敏并用半胱氨酸蛋白酶过敏原鼻内激发的小鼠过敏性气道炎症的作用。
  • DOI:
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KAMIJO Seiji;TAKAI Toshiro;HARA Mutsuko;MATSUDA Hironori;MATSUDA Akira;SAITO Hirohisa;NAKAE Susumu;ICHlKAWA Saori;OGAWA Hideoki;OKUMURA Ko
  • 通讯作者:
    OKUMURA Ko

MATSUDA Akira的其他文献

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{{ truncateString('MATSUDA Akira', 18)}}的其他基金

Methods and methodology for utilising an archaeological site after excavation as cultural resources of the local community
将发掘后的考古遗址作为当地社区文化资源利用的方法和方法
  • 批准号:
    16K03152
  • 财政年份:
    2016
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathophysiological Analysis of Atopic Glaucoma
特应性青光眼的病理生理分析
  • 批准号:
    24592652
  • 财政年份:
    2012
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Targeting of the nuclease-resistant functional oligonucleotides
核酸酶抗性功能性寡核苷酸的靶向
  • 批准号:
    23249008
  • 财政年份:
    2011
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Investigation into the molecular mechanism of the resolution of glucocorticoid resistance in lymphoma.
淋巴瘤糖皮质激素抵抗消退的分子机制研究。
  • 批准号:
    23880009
  • 财政年份:
    2011
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Investigation of epigenetic factors in the pathogenesis of glucocorticoid-induced glaucoma
糖皮质激素性青光眼发病机制中表观遗传因素的研究
  • 批准号:
    21592239
  • 财政年份:
    2009
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of multifunctional envelope-type nano device encapsulating highly nuclease resistant oligonucleotides
封装高核酸酶抗性寡核苷酸的多功能包膜型纳米器件的开发
  • 批准号:
    18109001
  • 财政年份:
    2006
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Design of Nucleosides Antitumor Agents
核苷类抗肿瘤药物的设计
  • 批准号:
    17016001
  • 财政年份:
    2005
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Basic Studies of Molecular Recognition by 4'-Thionucleic acids
4-硫代核酸分子识别的基础研究
  • 批准号:
    15209003
  • 财政年份:
    2003
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Design of Nuclease-resistant Antisense Molecules Based on the X-ray Structures of Some Nucleases
基于某些核酸酶X射线结构的抗核酸酶反义分子设计
  • 批准号:
    11557186
  • 财政年份:
    1999
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
A study on development of inhibitors for neuronal cell death
神经细胞死亡抑制剂的开发研究
  • 批准号:
    08557134
  • 财政年份:
    1996
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)

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