Revealing molecular detail of DNA triplexes to underpin antigene technology

揭示 DNA 三链体的分子细节以支持反基因技术

• 批准号: DP200102291
• 负责人: Prof Tara Pukala
• 金额: $ 31.6万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-07-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP200102291
• 关键词: Revealing; molecular; detail; DNA; triplexes

Variations from the classic DNA double helix structure are proposed to play key roles in a range of cellular processes, particularly gene regulation. However, the biological function and therapeutic potential of these unusual DNA structures are poorly explored, since the fundamental molecular details which govern their formation and interactions with cellular machinery are not well described. This project aims to develop innovative methods to investigate, and importantly modulate, DNA and RNA triple helix assembly, specificity and molecular interactions. Resulting insights will underpin novel approaches to gene regulation, principally in the context of designing new antibacterial agents to address the antibacterial resistance problem.

经典DNA双螺旋结构的变异被认为在一系列细胞过程中发挥关键作用，特别是基因调控。然而，这些不寻常的DNA结构的生物学功能和治疗潜力的探索很少，因为基本的分子细节，管理它们的形成和与细胞机制的相互作用没有得到很好的描述。该项目旨在开发创新的方法来研究，重要的是调节，DNA和RNA三螺旋组装，特异性和分子相互作用。由此产生的见解将支持新的基因调控方法，主要是在设计新的抗菌剂，以解决抗菌药物耐药性问题的背景下。