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Biochemical and genetic analyses of nonylphenol degradation metabolism in environmental bacteria

Biochemical and genetic analyses of nonylphenol degradation metabolism in environmental bacteria
环境细菌中壬基酚降解代谢的生化和遗传分析
批准号:
13480151
负责人:
OHTA Hiroyuki
金额:
$6.72万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2003

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中文摘要
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英文摘要
The results of this study are summarized as follows:1.Isolation and characterization of a nonylphenol-degrading bacterium from environmental samplesA Gram-negative bacterium (strain YT) able to degrade branched nonylphenol was isolated from a river sediment sample and characterized chemotaxonomically and phylogenetically. From the results, we propose that strain YT should be classified as a novel species, Sphingocium amiensis sp. nov.2.The metabolic pathway of nonylphenol degradation in S.amiense strain YT was analyzed. Two types of alkylphenol hydroxylases were identified in the strain3.Degradation profiles of alkylphenols by two nonyphenol-degrading bacteria, S.amiense YT and Sphingomonas cloacae JCM 10874, were analyzed.4.Genome sizes of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874, were determined.5.Two plasmids with the size of about 200 kb and 10 kb were found in each of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874. The 10 kb plasmid from S.Amiense YT was analyzed and applied to develop for useful vector among the Sphingomonas group bacteria from environments.
期刊论文(37)
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会议论文
Ikunaga, Y., Hasegawa, M., Kasahara, Y., Ohta, H.他共著: "Biogeochemical Aspects of Earth System and Bioremediation of Polluted Environments"The 16th International Symposium on Environmental Biogeochemistry and its OECD Session, Sendai, Japan. 124 (2003)
Ikunaga, Y.、Hasekawa, M.、Kasahara, Y.、Ohta, H. 等人:“地球系统的生物地球化学方面和污染环境的生物修复”第 16 届环境生物地球化学国际研讨会及其 OECD 会议,日本仙台124(2003)
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通讯作者:
生長陽子, 久留主泰朗, 笠原康裕, 太田寛行: "Sphingomonas sp. YT株の低濃度ノニルフェノールの分解性"第17回日本微生物生態学会講演要旨集. 78 (2001)
Yoko Seicho、Yasuo Kurushi、Yasuhiro Kasahara、Hiroyuki Ota:“鞘氨醇单胞菌 YT 菌株对低浓度壬基酚的降解性”第 17 届日本微生物生态学会论文集 78 (2001)。
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斎藤美有紀, 生長陽子, 太田寛行, 久留主泰朗: "Sphingomonas属細菌を宿主とするベクター系の開発"第19回日本微生物生態学会講演要旨集. 46 (2003)
Miyuki Saito、Yoko Seicho、Hiroyuki Ota、Yasuo Kurushi:“以鞘氨醇单胞菌为宿主的载体系统的开发”第 19 届日本微生物生态学会论文集 46 (2003)。
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