Biochemical and genetic analyses of nonylphenol degradation metabolism in environmental bacteria

环境细菌中壬基酚降解代谢的生化和遗传分析

• 批准号: 13480151
• 负责人: OHTA Hiroyuki
• 金额: $ 6.72万
• 依托单位: IBARAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13480151/
• 关键词: nonylphenol; endocrine disruptors; POP-degrading bacteria; Sphingobium; Sphingomanas; alkylphenol hydroxylase; degradation metabolism; スフィンゴビウム・アミエンセ; オクチルフェノール; 分解菌の環境動態; スフィンゴモナス属細菌; 分解菌の動態

The results of this study are summarized as follows:1.Isolation and characterization of a nonylphenol-degrading bacterium from environmental samplesA Gram-negative bacterium (strain YT) able to degrade branched nonylphenol was isolated from a river sediment sample and characterized chemotaxonomically and phylogenetically. From the results, we propose that strain YT should be classified as a novel species, Sphingocium amiensis sp. nov.2.The metabolic pathway of nonylphenol degradation in S.amiense strain YT was analyzed. Two types of alkylphenol hydroxylases were identified in the strain3.Degradation profiles of alkylphenols by two nonyphenol-degrading bacteria, S.amiense YT and Sphingomonas cloacae JCM 10874, were analyzed.4.Genome sizes of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874, were determined.5.Two plasmids with the size of about 200 kb and 10 kb were found in each of two nonylphenol-degrading bacteria, S.Amiense YT and Sphingomonas cloacae JCM 10874. The 10 kb plasmid from S.Amiense YT was analyzed and applied to develop for useful vector among the Sphingomonas group bacteria from environments.

本文的研究结果总结如下：1。从河流沉积物样品中分离到一株能降解支链壬基酚的革兰氏阴性菌（菌株YT），并对其进行了化学分类和系统发育鉴定。从结果来看，我们建议将菌株YT归类为新种Sphingocium amiensis sp. nov.2。分析了亚胺s.a amiense菌株YT降解壬基酚的代谢途径。在菌株3中鉴定出两种类型的烷基酚羟化酶。3 .分析了两种非酚降解菌s.a amiense YT和阴沟鞘单胞菌JCM 10874对烷基酚的降解特性。测定了两种壬基酚降解菌S.Amiense YT和阴沟鞘单胞菌JCM 10874的基因组大小。在amiense s.a iense YT和Sphingomonas cloacae JCM 10874两种降解硝基酚的细菌中分别发现了两个大小约为200kb和10kb的质粒。分析了amiense YT的10 kb质粒，并将其用于环境鞘氨单胞菌群细菌的有用载体开发。

项目成果

Ikunaga, Y., Hasegawa, M., Kasahara, Y., Ohta, H.他共著: "Biogeochemical Aspects of Earth System and Bioremediation of Polluted Environments"The 16th International Symposium on Environmental Biogeochemistry and its OECD Session, Sendai, Japan. 124 (2003)

Ikunaga, Y.、Hasekawa, M.、Kasahara, Y.、Ohta, H. 等人：“地球系统的生物地球化学方面和污染环境的生物修复”第 16 届环境生物地球化学国际研讨会及其 OECD 会议，日本仙台124（2003）

生長陽子, 久留主泰朗, 笠原康裕, 太田寛行: "Sphingomonas sp. YT株の低濃度ノニルフェノールの分解性"第17回日本微生物生態学会講演要旨集. 78 (2001)

Yoko Seicho、Yasuo Kurushi、Yasuhiro Kasahara、Hiroyuki Ota：“鞘氨醇单胞菌 YT 菌株对低浓度壬基酚的降解性”第 17 届日本微生物生态学会论文集 78 (2001)。

斎藤美有紀, 生長陽子, 太田寛行, 久留主泰朗: "Sphingomonas属細菌を宿主とするベクター系の開発"第19回日本微生物生態学会講演要旨集. 46 (2003)

Miyuki Saito、Yoko Seicho、Hiroyuki Ota、Yasuo Kurushi：“以鞘氨醇单胞菌为宿主的载体系统的开发”第 19 届日本微生物生态学会论文集 46 (2003)。

Ohta, H., Takahaara, Y., Ikunaga, Y., Ushiba, Y., Miyakawa, S., Hasegawa, M., Kasahara, Y., Kurusu, Y., Ono, K., Kubota, T.: "Environmental pollution by nonylphenol and its relative compounds and their degrading microbes: current knowledge about the pollu

太田 H.、高原 Y.、生永 Y.、牛芝 Y.、宫川 S.、长谷川 M.、笠原 Y.、来栖 Y.、小野 K.、久保田 T.：

太田寛行, 高原義治, 生長陽子, 牛場裕司, 宮川修一, 長谷川守文, 笠原康裕, 久留主泰朗, 小野勝道, 久保田俊夫: "ノニルフェノール類化合物質による環境汚染と分解微生物:土壌における実態の解明に向けて"日本微生物生態学会誌. 17巻・2号. 29-37 (2002)

Hiroyuki Ota、Yoshiharu Takahara、Yoko Icho、Yuji Ushiba、Shuichi Miyakawa、Morifumi Hasekawa、Yasuhiro Kasahara、Yasuo Kurushi、Katsumichi Ono、Toshio Kubota：“壬基酚化合物和降解微生物造成的环境污染：土壤中实际情况的澄清”杂志日本微生物生态学会，第 17 卷，第 2 期，29-37 (2002)