Re-examination on two different biosynthetic pathways of galactolipids in higher plants using molecular cell biological technique.

利用分子细胞生物学技术重新研究高等植物中半乳糖脂的两种不同生物合成途径。

• 批准号: 11640644
• 负责人: OHTA Hiroyuki
• 金额: $ 2.3万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640644/
• 关键词: Chloroplast; Glycolipid; Glycosyltransferase; Thylakoid; Monogalactosyldiacylglycerol; モノガラクトシルジアシルブリセロール; モノガラクトシルジアシルグリセロール

1) Three different types of MGDG synthase genes (AtMGD1, AtMGD2, AtMGD3) were identified in Arabidopsis thaliana.2) Tissue specificity of gene expression for three MGDG synthase genes was determined.3) Enzymatic property of three MGDG synthases was determined.4) Subcellular localization of three MGDG synthases was determined.5) According to the results described above, we classified three MGDG synthases into two types, namely type A (AtMGD1) and type B (AtMGD2, AtMGD3). Role of the two types in two different biosynthetic pathways in galactolipid synthesis was discussed, and their distinct roles were proposed.

1)三种不同类型的MGDG合酶基因（AtMGD 1，AtMGD 2，AtMGD 3）; 2）确定了3个MGDG合成酶基因表达的组织特异性; 3）确定了3个MGDG脱氢酶的酶学性质; 4）确定了3个MGDG脱氢酶的亚细胞定位; 5）根据上述结果，我们将3个MGDG脱氢酶分为两种类型，即A型（AtMGD 1）和B型（AtMGD 2，AtMGD 3）。讨论了这两种类型在半乳糖脂合成的两种不同生物合成途径中的作用，并提出了它们的不同作用。

项目成果

Awai, K., Marechal, E., Block, M.A, Brun, D., Masuda, T., Shimada, H., Takamiya, K., Ohta, H., Joyard, J.: "Two types of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and no

Awai, K.、Marechal, E.、Block, M.A、Brun, D.、Masuda, T.、Shimada, H.、Takamiya, K.、Ohta, H.、Joyard, J.：“两种类型的 MGDG 合酶

Ohta, H., Awai, K. and Takamiya, K.: "Glyceroglycolipids of photosynthetic organisms Their biosynthesis and evolutionary origin"Trends In Glycoscience and Glycotechnology. 12(66). 241-253 (2001)

Ohta, H.、Awai, K. 和 Takamiya, K.：“光合生物的甘油糖脂的生物合成和进化起源”糖科学和糖技术的趋势。

Christine Miege: "Biochemical and topological properties of type A MGDG synthase,a spinach chloroplast envelope enzyme catalyzing the synthesis of both prokaryotic and eukaryotic MGDG"Eur.J.Biochem.. 265. 990-1001 (1999)

Christine Miege：“A 型 MGDG 合酶的生化和拓扑特性，一种菠菜叶绿体包膜酶，催化原核和真核 MGDG 的合成”Eur.J.Biochem.. 265. 990-1001 (1999)

Koichiro Awai et al.: "Two type of MGDG synthase genes, found widely in both 16:3 and 18:3 plants, differentially mediate galactolipid syntheses in photosynthetic and nonphotosynthetic tissues in Arabidopsis thaliana"Proc. Natl. Acad. Sci. USA. 98. 10960-

Koichiro Awai 等人：“在 16:3 和 18:3 植物中广泛发现的两种类型的 MGDG 合酶基因，在拟南芥的光合和非光合组织中差异介导半乳糖脂合成”Proc.

Muhammed Gad et al.: "Accumulation of Plant Galactolipid Affects Cell Morphology of Esherichia celi"Biochem. Biophys. Res. Comm.. 286. 114-118 (2001)

Muhammed Gad 等人：“植物半乳糖脂的积累影响大肠杆菌的细胞形态”Biochem。