Re-examination on two different biosynthetic pathways of galactolipids in higher plants using molecular cell biological technique.

1)在拟南芥中鉴定了3种不同类型的MGDG合成酶基因(AtMGD1、AtMGD2、AtMGD3)。2)确定了3种MGDG合成酶基因表达的组织特异性。3)确定了3种MGDG合成酶的酶学性质。4)确定了3种MGDG合成酶的亚细胞定位。5)根据上述结果，我们将3种MGDG合成酶分为A型(AtMGD1)和B型(AtMGD2、AtMGD3)。讨论了这两种类型在两种不同的生物合成途径中的作用，并提出了它们各自的作用。

1) Three different types of MGDG synthase genes (AtMGD1, AtMGD2, AtMGD3) were identified in Arabidopsis thaliana.2) Tissue specificity of gene expression for three MGDG synthase genes was determined.3) Enzymatic property of three MGDG synthases was determined.4) Subcellular localization of three MGDG synthases was determined.5) According to the results described above, we classified three MGDG synthases into two types, namely type A (AtMGD1) and type B (AtMGD2, AtMGD3). Role of the two types in two different biosynthetic pathways in galactolipid synthesis was discussed, and their distinct roles were proposed.