Development of core techniques for microbial endosymbiosis engineering

本研究旨在开发一种将有益细菌内化到真菌细胞中的新技术，为微生物内共生工程做出贡献。为了提供细菌受体真菌，发明了从具有EB的真菌制备无内细菌（EB）的真菌的技术。此外，从具有EB的土壤真菌Morea elongta中回收EB组分，并测定其基因组序列。生物合成途径预测显示，缺乏硫代谢途径，这表明该内生细菌对真菌的严格依赖。

This study aimed to develop a new technique for the internalization of a useful bacterium into fungal cells, contributing to microbial endosymbiosis engineering. To provide a bacterium-recipient fungus, a technique of making an endobacterium (EB)-free fungus from an EB-possessing fungus was invented. Further, an EB fraction was rtiellasuccessfully recovered from an EB-possessing soil fungus, Morea elongta and its genome sequence was determined. Biosynthetic pathway prediction revealed the lack of sulfur metabolism pathways, suggesting the strict dependence of this endobacterium on the fungus.