Photoacoustic tomography of fluorescent contrast agents using pump-probe-excitation

生物医学光声（PA）断层成像是基于通过吸收强度调制光在组织中产生超声。由于血红蛋白在可见光和近红外波长范围内的主要吸收，可以采集软组织中血管网络的高分辨率3D图像。PA效应还提供与外源性造影剂的对比。目前用于从PA图像确定空间对比剂分布的方法在其灵敏度、特异性和一般有效性方面受到限制。在这个项目中，将开发新的实验和数值方法检测荧光团的泵浦-探测激发，并与精确的数值方法相结合，开辟了广泛的新的应用，在多路PA成像的生物物理和生化参数。

Biomedical photoacoustic (PA) tomography is based on the generation of ultrasound in tissue via the absorption of intensity-modulated light. Due to the dominant absorption of haemoglobin in the visible and near infrared wavelength range, high-resolution 3D images of the vascular networks in soft tissue can be acquired. The PA effect also provides contrast to exogenous contrast agents. Current methods for determining the spatial contrast agent distribution from PA images are limited in their sensitivity, specificity and general validity. In this project, novel experimental and numerical approaches for the detection of fluorophores by pump-probe excitation will be developed and combined with accurate numerical methods to open up a wide range of new applications in multiplexed PA imaging of biophysical and biochemical parameters.