Development of genetic and microbiological quality control system of mouse embryonic stem cell lines.

[导言]我们报道了染色体(Chr.)ICLAS监测中心发现异常和嵌合小鼠ES细胞系的生殖系传播(GT)。[材料与方法]从18个机构获得367株小鼠ES细胞系。用Giemsa染色和QH染色进行核型分析，并检测嵌合小鼠ES细胞系的GT。[结果](1)67.0%的机构(13个机构，246条线路)CHR正常。15.5%(8个机构，57条线路)、9.0%(6个机构，33条线路)和3.8%(4个机构，14条线路)分别出现41、42和39个Chr.(2)携带40个Chr.s的36个ES细胞系中有4个出现核型异常。其中1例核型为[40，X，+mar]。携带41个Chr.s的5个ES细胞系的核型包括[41，XY，+8(3个系)]、[41，XY，+18(1个系)]和[41，XY，+11(1个系)]。携带42个Chr.s的ES细胞系的核型包括[42，XY，+8，+11(2个系)]和[42，XY，+6，+8(1个系)]。(3)在2个ES细胞系中观察到GT，其CHR分别为正常的80%和88%。数字。[讨论](1)Chr.ES细胞系(33%)常出现数量异常。(2)在核型异常的细胞中，以携带8、11三体的ES细胞为主。(3)建议即使是携带40个Chr.s的ES细胞系也应进行核型分析。

[Introduction] We report chromosomal(chr.) abnormalities found in the ICLAS Monitoring Center and germline transmission(GT) by ES cell lines in chimeric mice. [Materials and methods] A total of 367 mouse ES cell lines were obtained from 18 institutions. Karyotype analyses were performed by Giemsa staining and QH staining, and GT by the ES cell lines in chimeric mice was examined. [Results](1)Among the institusions, 67.0%(13 institutions, 246 lines) showed normal chr. numbers, and 15.5% (8 institutions, 57 lines), 9.0% (6 institutions, 33 lines) and 3.8%(4 institutions, 14 lines) showed 41, 42, and 39 chr.s, respectively. (2)4 of 36 ES cell lines carrying 40 chr.s showed karyotyping abnormalities. The karyotype of 1 out of 4 was [40, X, +mar]. The karyotypes of 5 ES cell lines carrying 41 chr.s included [41, XY, +8(3 lines)],[41, XY, +18(1 line)] and [41, XY, +11(1 line)]. The karyotypes of ES cell lines carrying 42 chr.s included [42, XY, +8, +11(2 lines)] and [42, XY, +6, +8(1 line)]. (3)GT was observed in 2 ES cell lines exhibiting 80 and 88% of normal chr. numbers. [Discussion](1)Chr. number abnormalities occur frequently in ES cell lines (33%). (2)Among the cells carrying abnormal karyotypes, ES cells carrying trisomy 8 and 11 were dominant. (3)It was suggested that karyotyping should be performed even in ES cell lines carrying 40 chr.s.