Specific and quantitative detection of LAMP products from pathogens of laboratory animals using a newly developed electrochemical DNA chip.

使用新开发的电化学 DNA 芯片对实验动物病原体的 LAMP 产物进行特异性和定量检测。

• 批准号: 20500381
• 负责人: GOTO Kazuo
• 金额: $ 2.83万
• 依托单位: Teikyo University (2009-2010)Central Institute for Experimental Animals (2008)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500381/
• 关键词: ヘリコバクター; DNAチップ; LAMP法; PCR法; Bacillus subtilis; Lactobacillus casei; NCマウス; マウスノロウイルス; PCR; 病原体; マウス

We developed a microfabricated electrochemical DNA chip for detection of LAMP/PCR products from 16S rRNA sequences of Helicobacter spp. This chip does not require DNA labeling, and the hybridization signal can be detected as an anodic current. The average anodic currents of 5 (H.bilis) and 8 (H.hepaticus) PCR positive samples derived from feces of spontaneously infected mice (Cp, Hb and Hh) were 27.9±7.2, 31.9±8.1, 29.3±10.1, and 27.6±3.0 nA, respectively. On the other hand, the average anodic currents of 27 (Hb) and 18 (Hh), PCR negative samples were 3.7±2.4 and - 1.0±1.7, and nA, respectively. The anodic current increased with increasing concentrations of pathogens. For experimentally infected samples, the results of PCR/electrophoresis were in complete accord with those of this system when anodic currents of 8.5 (Hb) and 2.4 (Hh) nA were taken as the cut-off value. The results suggested that the electrochemical DNA chip system is useful for specific and quantitative detection of LAMP/PCR products.

我们研制了一种用于检测幽门螺杆菌16 SrRNA序列LAMP/PCR产物的电化学DNA芯片。这种芯片不需要DNA标记，杂交信号可以作为阳极电流检测。5例胆汁血吸虫和8例肝血吸虫阳性标本的平均阳极电流分别为27.9±7.2、31.9±8.1、29.3±10.1和27.6±3.0 nA。另一方面，27个（Hb）和18个（Hh）PCR阴性样品的平均阳极电流分别为3.7±2.4和-1.0 ±1.7和nA。阳极电流随着病原体浓度的增加而增加。对于实验感染的样品，当阳极电流为8.5（Hb）和2.4（Hh）nA时，PCR/电泳结果与本系统的结果完全雅阁。结果表明，电化学DNA芯片系统可用于LAMP/PCR产物的特异性和定量检测。

项目成果

Study of a Bordetella Hindi isolate from a laboratory mouse

从实验室小鼠中分离出的印地博德特氏菌的研究

• 发表时间: 2008
• 期刊: Comp.Med. 58
• 作者: Hayashimoto N.;Goto K.;et.;al.
• 通讯作者: al.

Morphological and sequence analysis of Mycoplasma sp. isolated from the oral cavity of a house musk shrew (Suncus murinus).

支原体的形态学和序列分析。

• 发表时间: 2010
• 期刊: Journal of Veterinary Medical Science
• 影响因子: 1.2
• 作者: K. Goto;M. Yasuda;N. Hayashimoto;S. Ebukuro
• 通讯作者: S. Ebukuro

characteristic karyotype abnormalities including robertsonian translocations found in eight mouse embryonic stem cell lines

在八个小鼠胚胎干细胞系中发现特征性核型异常，包括罗伯逊易位

• 发表时间: 2008
• 期刊: Chromosome Sci. 11
• 作者: Sugawara A.;Goto K.;et.;al.
• 通讯作者: al.

電流検出型DNAチップを用いた実験動物感染症モニタリングシステムの開発

电流检测型DNA芯片实验动物传染病监测系统的研制

• 发表时间: 2008
• 作者: 堀内秀紀;後藤一雄;他
• 通讯作者: 他

わが国の実験用マウスにおけるマウスノロウイルスの汚染状況

日本实验室小鼠诺如病毒污染状况

• 发表时间: 2009
• 期刊: LABIO 21 38
• 作者: Setsuie;R.;et al.;Ken-ichiro Kubo;後藤一雄
• 通讯作者: 後藤一雄