Analysis of Immnological Response in the Stomach of Suncus murinus against Newly Isolated Helicgbacter Species.

鼠鼯胃对新分离螺杆菌种的免疫反应分析。

• 批准号: 11680829
• 负责人: GOTO Kazuo
• 金额: $ 2.05万
• 依托单位: Central Institute for Experimental Anim als
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680829/
• 关键词: CDNA; Gastritis; Heticobacter; Mononuclear cell; Suncus murinua; 疫学調査; マウス; ラット; 抗生物質; 病原性

In 1998, we isolated novel Helicobacter species from the stomach of all Suncus murinus (SUN line) maintained in our facility, and the isolates correlated with the presence of histologic lesions of chronic gastritis in the animals (Curr Microbiol 37 : 44-51, 1998). In this study, immunological response in the stomach of Suncus murinus to the Helicobacter species (Helicobacter suncus JM1459) was analysed.Gastritis was observed in stomachs of all Suncus murinus naturally and/or experimentally infected with H. suncus. The gastritis consisted of mononuclear cells but neutrophilia was not observed. Ten clones from the eDNA library constructed from stomachs with gastritis of Suncus murinus infected with H. suncus" were strongly expressed in stomachs with gastritis but not in stomachs without gastritis.Our results suggested that mononuclear cells play an important role in the host immune response in stomachs of Suncus murinus against Helicobacter species, and the gastritis related gene was obtained from the cDNA library constructed in this study. Clarification of details of immunological response in the stomachs of Suncus murinus requires analysis of the clones.

1998 年，我们从我们设施中饲养的所有 Suncus murinus（SUN 系）的胃中分离出新的螺杆菌，并且这些分离株与动物中慢性胃炎的组织学病变的存在相关（Curr Microbiol 37：44-51，1998）。在这项研究中，分析了 Suncus murinus 胃部对螺杆菌 (Helicobacter suncus JM1459) 的免疫反应。在自然和/或实验感染 H. suncus 的所有 Suncus murinus 胃中均观察到胃炎。胃炎由单核细胞组成，但未观察到中性粒细胞增多。感染H. suncus的Suncus murinus胃炎胃构建的eDNA文库中的10个克隆在患有胃炎的胃中强烈表达，但在没有胃炎的胃中没有强烈表达。我们的结果表明，单核细胞在Suncus murinus胃中针对螺杆菌的宿主免疫反应中发挥着重要作用，并且胃炎相关基因是从本研究构建的cDNA文库中获得的。 要了解鼠鼯鼠胃中免疫反应的细节，需要对克隆进行分析。

项目成果

Keiji Ishii, Susumu Ebukuro, et al.: "Diurnal fluctuations of heart rate, body temperature and locomotor activity in the house musk shrew (Suncus murinus)"Exp.Anim. 51. 57-62 (2002)

Keiji Ishii、Susumu Ebukuro 等人：“家麝鼩 (Suncus murinus) 心率、体温和运动活动的昼夜波动”Exp.Anim。

Kazuo Goto, et al.: "Contamination of transplantable human tumor-bearing lines by Helicobacter hepaticus and its elimination"J.Clin.Microbiol.. 39. 3703-3704 (2001)

Kazuo Goto 等人：“肝螺杆菌对可移植人类肿瘤荷瘤系的污染及其消除”J.Clin.Microbiol.. 39. 3703-3704 (2001)

Hisao Ito, Susumu Ebukuro, et al.: "Immunohistochemical demonstration of c-fos protein in neurons of the medulla oblongata of the musk shrew after veratrine administration"Exp.Anim.. 51. 19-25 (2002)

Hisao Ito、Susumu Ebukuro 等人：“给予藜芦碱后麝鼩延髓神经元中 c-fos 蛋白的免疫组织化学演示”Exp.Anim.. 51. 19-25 (2002)

Kazuo Goto, et al.: "Pathogenicity of Helicobacter species isolated from the stomach of the house musk shrew (Suncus murinus)"Lab.Anim.Sci.. 50. 73-77 (2000)

Kazuo Goto 等人：“从家麝鼩 (Suncus murinus) 胃中分离出的螺杆菌的致病性”Lab.Anim.Sci.. 50. 73-77 (2000)

Keiji Ishii, Masahiro Uchino, Masayoshi Kuwahara, Hirokazu Tsubone, and Susumu Ebukuro: "Diurnal fluctuations of heart rate, body temperature and locomotor activity in the house musk shrew (Suncus murinus)."Exp. Anim.. 51. 57-62 (2002)

Keiji Ishii、Masahiro Uchino、Masayoshi Kuwahara、Hirokazu Tsubone 和 Susumu Ebukuro：“家麝鼩 (Suncus murinus) 心率、体温和运动活动的昼夜波动。”Exp。