Multifunctional Microscope for Spinning Disk, TIRF, STORM and SRRF Acquisition

所申请的设备是一种高分辨率显微镜，在一个设备中结合了各种记录模式/显微镜技术，部分可以并行使用。它的主要目的是使明斯特大学的传染病研究人员能够对风险组2病原体的样本进行显微镜检查，以解决各种各样的问题，特别是在活细胞中。这些包括用于质膜记录的全内反射显微镜（TIRF），用于细胞和类器官上快速活细胞记录的共焦旋转圆盘显微镜，以及所谓的超分辨率技术，如随机光学重建显微镜（STORM）和超分辨率径向波动（SRRF），以克服阿贝限制。其目的是使明斯特成像网络，特别是细胞病毒学研究所能够使用活细胞中最先进的方法处理感染学中的微观问题。

The device applied for is a high-resolution microscope that combines various recording modes / microscopy techniques in one device, which in part can be used in parallel. It is primarily intended to enable infectious researchers at Münster University to perform microscopy with samples of risk group 2 pathogenic agents to address a wide variety of issues, particularly in living cells. These include total internal reflection microscopy (TIRF) for recordings on the plasma membrane, confocal spinning disk microscopy for rapid live cell recordings on cells and organoids, as well as so-called super resolution techniques such as stochastic optical reconstruction microscopy (STORM) and super resolution radial fluctuations (SRRF) to overcome the Abbe limits. The aim is to enable the Münster Imaging Network and, in particular, the Institute of Cellular Virology to deal with microscopic questions in infectiology using state-of-the-art methods in living cells.