Evaluation of Combined Toxic Potency of Xenobiotics and the Decreasing Effect of Edible Plants

将双酚A（BPA）和亚硝酸钠混合物溶于pH3.0的醋酸盐缓冲液中，37 ℃孵育60min，分离得到一种新的含羟基和偶氮基的诱变剂。当使用吗啉和亚硝酸钠的混合物时，生成亚硝基吗啉（NMo）。但茶叶提取物的加入降低了诱变性和NMo含量。这些数据表明，环境化学品和亚硝酸钠存在于蔬菜和唾液中的联合作用，通过比较微核试验和彗星试验的遗传毒性，用啮齿动物和鱼，金鱼是一个有用的候选人作为实验动物。

A mixture of bisphenol A (BPA) and sodium nitrite was dissolved in acetate buffer (pH3.0) and incubated at 37℃ for 60 min, a new mutagens having hydroxyl and azo groups was isolated. When a mixture of morpholine and sodium nitrite was used, nitrosomorpholine (NMo) was produced. But the addition of tea extract decreased the mutagnnicity and the content of NMo. These data demonstrated the combined effects between environmental chemicals and sodium nitrite that is present in vegetables and saliva were observed.By comparison of the genotoxicity of micronucleus test and comet assay using rodent or fish, goldfish was found to be a useful candidate as the experimental animal.