Combined effect of sawa-wasabi and green tea to H. pylori and the related epidemiological study

以前，我们报道了山葵(Wasabia Japan Onica)的几种含有抗菌效力的功能成分。山葵是我县的一种传统香料，也是一种特产。我们还研究了日本传统饮料绿茶(Camelia Sinens)的抗氧化和抗突变作用。本文旨在阐明山葵和绿茶对幽门螺杆菌诱导的胃癌的联合预防作用。研究结果表明：(1)山葵叶的乙醚提取物和水溶物具有较强的抗氧化活性；(2)山葵的根茎和叶对幽门螺杆菌具有生长抑制和消毒作用；(3)山葵叶提取物具有较强的清除自由基作用。(4)山葵叶提取物和绿茶提取物的混合提取物对幽门螺杆菌有较强的杀菌作用。另外。(5)给幽门螺杆菌口服山葵叶提取物。注射沙土鼠后，胃粘膜细菌数、损伤评分和点状出血评分均显著降低。(6)流行病学研究表明，山葵、绿茶胃癌SMR产地均低于对照地区。这些实验数据证明了山葵和绿茶对幽门螺杆菌的联合预防作用。感染是可以预料到的。

In previously, we reported several functional properties containing antibacterial potency of Sawa-wasabi (Wasabia japonica), a traditional spice and a special product of our prefecture. We also studied on antioxidative and antimutagenic effects of green tea (Camelia sinenses) a traditional beverage of Japan.In this paper, our purpose was to elucidate the combined preventive effect of wasabi and green tea to Helicobacter pylori induced stomach cancer. The epidemiological data between incidence of stomach cancer and intake of wasabi and/or green tea was required in this experiments.We obtained this following results from this study.(1) The ether extracts and water soluble fraction of wasabi leaves showed strong antioxdative activity.(2) The rhizomes and leaves of wasabi showed growth inhibition and disinfection to H.pylori.(3) Wasabi leaf extract showed strong radical scavenging activity.(4) The mixture of wasabi and green tea extracts exhibited bactericidal effect to H.pylori. additively.(5) By oral administration of wasabi leaf extract to H.pylori. injected Mongolian gerbils, the number of bacteria, injury and petechial hemorrhange scores of gastric mucosa was significantly decreased.(6) By epidemiological studies, the producing areas of wasabi and green tea stomach cancer SMR was lower than those of control areas. These experimental data demonstrates that the combined preventive effect between wasabi and green tea to H.pylori. infection might be expected.