Plant components modifies the absorption, metabolism and excretion of xenobiotics.

植物成分改变外源物质的吸收、代谢和排泄。

• 批准号: 11680136
• 负责人: KINAE Naohide
• 金额: $ 1.41万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680136/
• 关键词: antioxidative potency; antimutaenicity; radical scavenging pote; catechin; sea lettuce; flavonoid; metabolism; adsorption; MX; ベンツ(a)ピレン; 吸収; 排泄; 抗酸化物質; 食物繊維; ベンズ(a)ピレン

In this study several effective components were isolated from green tea and seaweeds, which show antioxidative, radical scavenging and antimutagenic activities. The modification potency of these components to absorption, metabolism and excretion of xenobiotics were examined in vivo and in vitro.1. The methanol extract of sea lettuce (Ulva pertusa kjellman) showed antioxidative activity in a dose dependent manner at the range of 0.5 to 5.0 mg/ml. But the potency of water extract was 20 % compared to that of the methanol extract. And radical scavenging activity (ED_50) of the methanol extract was 0.7-1.6 mg/ml. Moreover, the methanol extract inhibited the mutagenicity of MelQx and Trp P-1 which were formed in cooked meat arid fish.2. Green tea extract suppressed the mutagenicity of nitrosomorpholine (34% in 0.5 mg/plate, 70 % in l.0 mg/plate). Green tea extract also inhibited the formation of nitrosomorpholine in rat oral administered sodium nitrite and morpholine.3. Several flavonoids containing quercetin and rutin, and food fiber such as chitin, chitosan and cellulose showed the strong adsorption potency to DOT having carcinogenicity and endocrine disrupting activity.From these data, the intake of plant components including flavonoids may. be beneficial tool to detoxify many xenobiotics by modification of absorption, metabolism and excretion steps.

从绿茶和海藻中分离出具有抗氧化、清除自由基和抗诱变活性的有效成分。在体内和体外研究了这些成分对外源药物吸收、代谢和排泄的修饰作用。海莴苣（Ulva pertusa kjellman）甲醇提取物的抗氧化活性在0.5 ~ 5.0 mg/ml范围内呈剂量依赖性。但水提取物的效力比甲醇提取物的效力高20%。甲醇提取物的自由基清除活性（ED_50）为0.7 ~ 1.6 mg/ml。甲醇提取物对熟肉和熟鱼中形成的MelQx和Trp -1的致突变性有抑制作用。绿茶提取物对亚硝基somoroline的致突变性有抑制作用（0.5 mg/板时为34%，1.0 mg/板时为70%）。绿茶提取物还能抑制大鼠口服亚硝酸钠和吗啡形成亚硝酸钠。含有槲皮素、芦丁的几种黄酮类化合物和几丁质、壳聚糖、纤维素等食物纤维对DOT具有较强的吸附能力，具有致癌性和内分泌干扰活性。从这些数据来看，包括类黄酮在内的植物成分的摄入可能。是通过改变吸收、代谢和排泄步骤来解毒许多外来生物的有益工具。

项目成果

Shimoi K.,Kinae N., et al: "Metabolic fate of luteolin and its functional activity at focal site."Biofactors. 12. 181-186 (2000)

Shimoi K.、Kinae N. 等人：“木犀草素的代谢命运及其在焦点部位的功能活性。”生物因子。

Arai Y,, Watanabe S., Kimira M., Onodera J., Chida A. Onodera H., Ikeno T., Shimoi K,. Mochizuki R., and Kinae N.: "Dietary intake of Nutrition flavonol, flavone and isoflavone in Japanese women : The quercetin intake inversely associated with the plasma

Arai Y,, Watanabe S., Kimira M., Onodera J., Chida A. Onodera H., Ikeno T., Shimoi K,.

Nishikawa A,Kinae N.et al.: "Promoting effects of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone on rat glandular stomach carcinogenesis initiated with N-methyl-N'-nitro-N-nitrosoguanidine"Cancer Res.,. 59(9). 2045-2049 (1999)

Nishikawa A,Kinae N.等人：“3-氯-4-(二氯甲基)-5-羟基-2(5H)-呋喃酮对 N-甲基-N-硝基引发的大鼠腺胃癌的促进作用

Miyake, Y.: "Identification and antioxidant activity of flavonoid metabolites in plasma and urine of eriocitrin-treated rats"J. Agric. Food Chem.. 48(8). 3217-3224 (2000)

Miyake，Y.：“圣草次苷治疗大鼠血浆和尿液中类黄酮代谢物的鉴定和抗氧化活性”J。

Kinae, N: "Functional properties of wasabi and horseradish"Bio-Factors. 13. 265-269 (2000)

Kinae, N：“芥末和辣根的功能特性”生物因子。