Scavenging Potency of Tea Extracts to Active Oxygen and Regulation of Aging via MAILLARD Reaction in vivo

(1)采用1，1-二苯基-2-苦肼法和5，5-二甲基吡咯啉-N-氧化物电子自旋共振法研究了几种茶叶提取物（绿色茶、波雷茶、路易波士茶）对自由基的清除作用。所有茶叶提取物对超氧阴离子均有较强的清除作用，清除活性大小依次为绿色茶、波雷茶和路易波士茶。(2)将茶提取物给予链脲佐菌素处理的糖尿病小鼠后，通过ELISA方法使用抗晚期糖基化终产物（AGE）抗体测定AGE含量的时间过程。绿色茶和坡雷茶对血浆AGE含量有明显的抑制作用。从绿色茶中提取的儿茶素和从坡垒茶中提取的黄酮类化合物被鉴定为抑制剂。(3)从亲和液相色谱法和酶联免疫吸附法的数据来看，糖尿病患者血浆中AGE的含量明显高于正常人。AGE含量与HbA_1有一定的相关性<1C>。

(1) The radical scavenging potency of several tea extracts (green tea, Polei tea, Rooibos tea) was examined by 1,1-diphenyl-2-picrylhydrazil method and electron spin resonance method using 5,5-dimethylpyrroline-N-oxide. All tea extract showed high scavenging effects on superoxide anion and the activity was in order of green tea, Polei tea and Rooibos tea.(2) After administration of tea extract to streptozotocin treated diabetic mice, time course of advanced glycation endproducts (AGE) content was determined by ELISA method using anti-AGE antibody. Green tea and Polei tea showed clear inhibitory effects on AGE content in plasma. Catechins from green tea and flavonoids from Polei tea were identified as inhibitors.(3) From the data of affinity-HPLC and ELISA,AGE contents in plasma of diabetic patients were significantly higher than those of normal subjects. A certain correlation was observed between AGE content and HbA_<1C>.