Elucidation of mechanism for spontaneous molecular-recognition in lipid non-bilayer membranes

脂质非双层膜自发分子识别机制的阐明

基本信息

  • 批准号:
    15550122
  • 负责人:
  • 金额:
    $ 2.43万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2005
  • 项目状态:
    已结题

项目摘要

In this study, we investigated the molecular recognition ability of lipid molecules in non-bilayer aggregates formed by phospholipids, that is, interdigitated gel and inverted hexagonal structures, by means of macroscopic methods such as differential scanning calorimetry and high-pressure light transmittance measurements and a microscopic method as a high-pressure fluorescent probe measurement. The obtained results are summarized as follows :1.The bilayer phase transitions for four kinds of N-methylated dipalmitoylphospatidylethanolamines with different-sized polar head groups under high pressure were examined. The stability and polymorphism of gel phases among the phospholipid bilayers were revealed.2.The barotropic phase transitions of asymmetric phosphatidylcholine bilayer membrane were observed. Interdigitation of asymmetric phosphatidylcholine was markedly different from those of symmetric ones.3.The fluorescence spectra of Prodan for the bilayer membranes of four kinds of diacylp … More hosphatidylcolines with varying acyl chain lengths were observed as a function of pressure. We speculated the location of the Prodan molecules in bilayer membranes in different phases from the shift of fluorescence spectra.4.The phase transition three kinds of phosphatidylcholine bilayer membranes in deuterium oxide and hydrogen oxide were examined. The substitution of hydrogen oxide by deuterium oxide produced a great effect on the transitions between gel phases and unstabilized the interdigitated gel phase.5.The fluorescence spectra of Prodan and Laurdan in bilayer membranes of phosphatidylcholine were observed as a function of pressure at constant temperature. In being formed the interdigitated gel phase, the Laurdan molecule was not squeezed out from the glycerol backbone region of phospholipid molecules because of the long acyl chain.6.The phase transition between the liquid crystal phase and the inverted hexagonal phase of dioleoylphosphatidylethanolamine bilayer membrane in aqueous NaCl solutions was investigated. We concluded that the transition can be regarded as the volume-driven transition for the reconstruction of molecular packing. Less
本研究通过差示扫描量热法、高压透光率测量等宏观方法和高压荧光探针测量等微观方法,研究了磷脂形成的非双层聚集体,即指间凝胶和倒六边形结构中脂类分子的分子识别能力。所得结果总结如下:1。研究了四种不同极性头基团大小的n -甲基化双棕榈酰基磷空间基乙醇胺在高压下的双层相变。揭示了磷脂双分子层凝胶相的稳定性和多态性。观察了不对称磷脂酰胆碱双层膜的正压相变。不对称磷脂酰胆碱与对称磷脂酰胆碱的互指性明显不同。研究了四种二酰基双分子膜上Prodan的荧光光谱随压力的变化规律。我们通过荧光光谱的位移推测了Prodan分子在不同相的双层膜中的位置。考察了三种磷脂酰胆碱双层膜在氧化氘和氧化氢中的相变。氧化氘取代氧化氢对凝胶相和不稳定的交错凝胶相之间的转变产生了很大的影响。在恒温条件下,观察了磷脂酰胆碱双层膜中Prodan和laudan的荧光光谱随压强的变化。在形成互指凝胶相时,由于长酰基链,Laurdan分子没有从磷脂分子的甘油主链区挤出。研究了二油酰基磷脂酰乙醇胺双层膜在NaCl水溶液中液晶相与倒六方相的相变。我们的结论是,这种转变可以看作是分子包装重建的体积驱动转变。少

项目成果

期刊论文数量(86)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
局所麻酔作用のモデル研究- 界面化学的手法による作業機構の解明-
局部麻醉作用的模型研究 - 利用表面化学方法阐明作用机制 -
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    H.Nishikawa;Y.Yasuda;T.Kodama;K.Kikuchi;I.Ikemoto;J.Yamada;H.Oshio;Nobuhiro Watanabe et al.;松木 均
  • 通讯作者:
    松木 均
Model Study on Local Anesthetic Action-Elucidation of Action Mechanism by Surface-Chemical Methods-
局部麻醉作用模型研究-通过表面化学方法阐明作用机制-
Partition Coefficients of Local Anesthetic into Lipid Bilayer Membranes containing unsaturated acyl chain
局麻药在含不饱和酰基链的脂质双层膜中的分配系数
脂質二分子膜への局所麻酔薬の分配に及ぼすコレステロールの影響
胆固醇对局麻药分配至脂质双层膜的影响
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T.Nakabayashi;T.Ishida;T.Nogami;Y.Teki;松岡和彦
  • 通讯作者:
    松岡和彦
Recognition for Long-Chain Fatty Acids by Dipalmitoylphosphatidylcholine Bilayer Membranes
二棕榈酰磷脂酰胆碱双层膜识别长链脂肪酸
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MATSUKI Hitoshi其他文献

MATSUKI Hitoshi的其他文献

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{{ truncateString('MATSUKI Hitoshi', 18)}}的其他基金

Bilayer characterization of analog lipids having modified module structure
具有修饰模块结构的类似脂质的双层表征
  • 批准号:
    19K05404
  • 财政年份:
    2019
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Are inhalation anesthetics really receptor-binding drugs? : calorimetry of firefly luciferase
吸入麻醉药真的是受体结合药物吗?
  • 批准号:
    24659698
  • 财政年份:
    2012
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research

相似海外基金

Elucidation of structure and polarity inside lipid bilayer membranes as studied by laser spectroscopy of lipid bilayer membrane nanodiscs
通过脂质双层膜纳米盘的激光光谱研究阐明脂质双层膜内部的结构和极性
  • 批准号:
    18K05043
  • 财政年份:
    2018
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Collaborative Proposal: Theoretical, computational, and experimental investigations on the interaction between a lipid bilayer membrane and a solid substrate or particle
合作提案:脂质双层膜与固体基质或颗粒之间相互作用的理论、计算和实验研究
  • 批准号:
    1614863
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Standard Grant
Collaborative Proposal: Theoretical, computational, and experimental investigations on the interaction between a lipid bilayer membrane and a solid substrate or particle
合作提案:脂质双层膜与固体基质或颗粒之间相互作用的理论、计算和实验研究
  • 批准号:
    1614892
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Standard Grant
Collaborative Proposal: Theoretical, computational, and experimental investigations on the interaction between a lipid bilayer membrane and a solid substrate or particle
合作提案:脂质双层膜与固体基质或颗粒之间相互作用的理论、计算和实验研究
  • 批准号:
    1614907
  • 财政年份:
    2016
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Standard Grant
Dynamics of lipid bilayer membrane induced by external stimuli
外部刺激诱导的脂质双层膜动力学
  • 批准号:
    15H03708
  • 财政年份:
    2015
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Real-time observation and biophysical analysis of the stability of lipid bilayer membrane
脂质双层膜稳定性的实时观察和生物物理分析
  • 批准号:
    24651134
  • 财政年份:
    2012
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of lipid bilayer membrane vesicles constructed by fat-soluble vitamin and application for cancer treatment
脂溶性维生素构建的脂质双层膜囊泡的研制及其在癌症治疗中的应用
  • 批准号:
    24790060
  • 财政年份:
    2012
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Controlled Formation of Meso-sized Metal Complex Crystals Using Lipid Bilayer Membrane
使用脂质双层膜控制形成介观尺寸金属络合物晶体
  • 批准号:
    23655055
  • 财政年份:
    2011
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Functional studies on steric structure of ion channels -supporting voltage-dependent fluctuations of voltage-sensor domains of ion channels in lipid bilayer membrane-
离子通道空间结构的功能研究 - 支持脂质双层膜中离子通道电压传感器域的电压依赖性波动 -
  • 批准号:
    17390056
  • 财政年份:
    2005
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Photocontrol of ion flux across a lipid bilayer membrane by visible light
可见光对穿过脂质双层膜的离子通量进行光控制
  • 批准号:
    15550142
  • 财政年份:
    2003
  • 资助金额:
    $ 2.43万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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