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Elucidation of mechanism for spontaneous molecular-recognition in lipid non-bilayer membranes

脂质非双层膜自发分子识别机制的阐明

基本信息

批准号：
15550122
负责人：
MATSUKI Hitoshi
金额：
$ 2.43万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15550122/
关键词：
Phospholipid Lipid bilayer membrane Phase transition Non-bilayer structure Interdigitated gel phase Hexagonal II phase High pressure Molecular recognition 相図コレステロール巨大ベシクル巨大単層ベシクル麻酔薬

项目摘要

In this study, we investigated the molecular recognition ability of lipid molecules in non-bilayer aggregates formed by phospholipids, that is, interdigitated gel and inverted hexagonal structures, by means of macroscopic methods such as differential scanning calorimetry and high-pressure light transmittance measurements and a microscopic method as a high-pressure fluorescent probe measurement. The obtained results are summarized as follows :1.The bilayer phase transitions for four kinds of N-methylated dipalmitoylphospatidylethanolamines with different-sized polar head groups under high pressure were examined. The stability and polymorphism of gel phases among the phospholipid bilayers were revealed.2.The barotropic phase transitions of asymmetric phosphatidylcholine bilayer membrane were observed. Interdigitation of asymmetric phosphatidylcholine was markedly different from those of symmetric ones.3.The fluorescence spectra of Prodan for the bilayer membranes of four kinds of diacylp … More hosphatidylcolines with varying acyl chain lengths were observed as a function of pressure. We speculated the location of the Prodan molecules in bilayer membranes in different phases from the shift of fluorescence spectra.4.The phase transition three kinds of phosphatidylcholine bilayer membranes in deuterium oxide and hydrogen oxide were examined. The substitution of hydrogen oxide by deuterium oxide produced a great effect on the transitions between gel phases and unstabilized the interdigitated gel phase.5.The fluorescence spectra of Prodan and Laurdan in bilayer membranes of phosphatidylcholine were observed as a function of pressure at constant temperature. In being formed the interdigitated gel phase, the Laurdan molecule was not squeezed out from the glycerol backbone region of phospholipid molecules because of the long acyl chain.6.The phase transition between the liquid crystal phase and the inverted hexagonal phase of dioleoylphosphatidylethanolamine bilayer membrane in aqueous NaCl solutions was investigated. We concluded that the transition can be regarded as the volume-driven transition for the reconstruction of molecular packing. Less

本研究通过差示扫描量热法、高压光透过率测量等宏观方法和高压荧光探针的微观测量方法，研究了磷脂形成的非双层聚集体--交错凝胶和倒六方结构中脂质分子的分子识别能力。主要研究结果如下：1.研究了四种不同大小的N-甲基二棕榈酰基磷酰乙醇胺在高压下的双层相变。揭示了不对称磷脂双层膜凝胶相的稳定性和多晶性。2.观察到不对称磷脂酰胆碱双层膜的正压相变。不对称磷脂酰胆碱与对称磷脂酰胆碱的交错反应明显不同。3.四种双酰基…双层膜的普罗丹荧光光谱随着压力的增加，更多的磷脂酰胆碱具有不同的酰基链长度。通过荧光光谱的漂移推测普罗丹分子在双层膜中的位置。4.研究了三种磷脂酰胆碱双层膜在氢氧化氢和重水中的相变。H_2O_2被H_2O_3取代对凝胶相间的转变有很大的影响，并且不稳定交指状凝胶相。5.在恒温下观察了普罗丹和劳丹在磷脂酰胆碱双层膜中的荧光光谱随压力的变化。在形成交错的凝胶相时，由于长的酰基链，Laurdan分子没有从磷脂分子的甘油主链区域被挤出。6.研究了双油酰磷脂酰乙醇胺双层膜在氯化钠溶液中的液晶相和反六方相之间的相变。我们的结论是，对于分子堆积的重建来说，这个转变可以看作是体积驱动的转变。较少