HRAM (High Resolution Accurate Mass)-Liquid Chromatography Hybrid Mass Spectrometer

活性药物成分，药物代谢产物，候选药物的合成和分析以及生物合成途径的研究，细菌多糖降解机制和天然物质的结构澄清，部分在复杂的基质中，是参与工作组的研究工作的重点。 上述所有问题都需要高分辨率的精确质量测量数据，这些数据以前可以使用该研究所运行的混合质谱仪系统收集。该系统在使用13年后不再运作，将在平等的基础上予以取代。由于具有显著提高的性能的液相色谱系统（超高效液相色谱）已经越来越多地建立，所以这次将采购这样的混合系统。 内部两个单四极杆系统和三重四极杆质谱仪补充了使用所需设备确定分析物精确质量的能力，其于2020年购买用于灵敏定量，且无法以所需精度测量高分辨率准确质量数据。上述-除了上述工作外，还进行了细胞分析，以研究生物活性和病理生理刺激，以及海洋生物技术领域的其他蛋白质组学项目。此外，该研究所还处理有关药物在生物体和模型系统中的运输，药物形式和耐药机制中活性成分的释放以及人类病原菌的毒力因子的问题，迄今为止尚未使用高分辨率精确的质量数据，但可以通过精确的质量测量获得更多的见解。所要求的设备配置旨在维持和丰富整个药学研究所的研究机会。研究目标是阐明钾通道调节剂的作用和副作用的分子和超分子基础，sirtuin抑制剂的鉴定和定量，药物代谢物以及人工酶产品混合物的研究和制备纯化，二肽和三肽转运蛋白的“转运体”的表征，来自非洲药用植物提取物的组分的结构表征，用于发现新的半胱氨酸天冬氨酸蛋白酶原-3激活剂的合成产物的表征，人类免疫细胞中未知脂质介质和来自白色念珠菌的新氧化脂质的鉴定，共生海洋生物的蛋白基因组学和细菌多糖降解的未知机制的澄清。

The synthesis and analysis of active pharmaceutical ingredients, drug metabolites, drug candidates as well as the investigation of biosynthesis pathways, mechanisms of bacterial polysaccharide degradation and structural clarification of natural substances, partly in complex matrices, are the focus of the research work of the participating working groups of the Institute of Pharmacy at the University of Greifswald. High resolution accurate mass measurement data are required for all of the above questions, which could previously be collected using a hybrid mass spectrometer system operated in the institute. The system, which is no longer operational after 13 years of use, is to be replaced on an equal footing. Since liquid chromatography systems with significantly increased performance (ultra high performance liquid chromatography) have become increasingly established, a such a hybrid system is to be procured this time. The ability to determine the exact mass of analytes with the requested device is complemented by the in-house two single-quadrupole systems and the triple quadrupole mass spectrometer, which was purchased in 2020 for sensitive quantification and with which no high resolution accurate mass data can be measured with the necessary accuracy.The above-mentioned works are supplemented on the one hand by cellular assays for the study of biological activity and pathophysiological stimuli as well as other proteomics projects in the field of marine biotechnology. In addition, the institute deals with questions about the transport of drugs in the organism and in model systems, the release of active ingredients from drug forms and resistance mechanisms and virulence factors of human pathogenic bacteria, for which no high resolution accurate mass data has been used so far, but for which additional insights could be gained by exact mass measurements. The requested device configuration is intended to maintain and enrich the research opportunities of the entire Institute of Pharmacy.Research objectives are to clarify the molecular and supramolecular basis of the effects and side effects of potassium channel modulators, identification and quantification of sirtuin inhibitors, drug metabolites and investigation and preparative purification of product mixtures of artificial enzymes, characterization of the "transportophore" of di- and tripeptide transporters, structural characterization of components from African medicinal plant extracts, characterization of synthesis products for the discovery of new procaspase-3 activators, identification of unknown lipid mediators in human immune cells and new oxylipins from Candida albicans, proteogenomics of symbiotic marine organisms and clarification of unknown mechanisms of bacterial polysaccharide degradation.