HRAM (High Resolution Accurate Mass)-Liquid Chromatography Hybrid Mass Spectrometer

HRAM(高分辨率精确质量)-液相色谱混合质谱仪

基本信息

  • 批准号:
    463304797
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    德国
  • 项目类别:
    Major Research Instrumentation
  • 财政年份:
    2021
  • 资助国家:
    德国
  • 起止时间:
    2020-12-31 至 无数据
  • 项目状态:
    未结题

项目摘要

The synthesis and analysis of active pharmaceutical ingredients, drug metabolites, drug candidates as well as the investigation of biosynthesis pathways, mechanisms of bacterial polysaccharide degradation and structural clarification of natural substances, partly in complex matrices, are the focus of the research work of the participating working groups of the Institute of Pharmacy at the University of Greifswald. High resolution accurate mass measurement data are required for all of the above questions, which could previously be collected using a hybrid mass spectrometer system operated in the institute. The system, which is no longer operational after 13 years of use, is to be replaced on an equal footing. Since liquid chromatography systems with significantly increased performance (ultra high performance liquid chromatography) have become increasingly established, a such a hybrid system is to be procured this time. The ability to determine the exact mass of analytes with the requested device is complemented by the in-house two single-quadrupole systems and the triple quadrupole mass spectrometer, which was purchased in 2020 for sensitive quantification and with which no high resolution accurate mass data can be measured with the necessary accuracy.The above-mentioned works are supplemented on the one hand by cellular assays for the study of biological activity and pathophysiological stimuli as well as other proteomics projects in the field of marine biotechnology. In addition, the institute deals with questions about the transport of drugs in the organism and in model systems, the release of active ingredients from drug forms and resistance mechanisms and virulence factors of human pathogenic bacteria, for which no high resolution accurate mass data has been used so far, but for which additional insights could be gained by exact mass measurements. The requested device configuration is intended to maintain and enrich the research opportunities of the entire Institute of Pharmacy.Research objectives are to clarify the molecular and supramolecular basis of the effects and side effects of potassium channel modulators, identification and quantification of sirtuin inhibitors, drug metabolites and investigation and preparative purification of product mixtures of artificial enzymes, characterization of the "transportophore" of di- and tripeptide transporters, structural characterization of components from African medicinal plant extracts, characterization of synthesis products for the discovery of new procaspase-3 activators, identification of unknown lipid mediators in human immune cells and new oxylipins from Candida albicans, proteogenomics of symbiotic marine organisms and clarification of unknown mechanisms of bacterial polysaccharide degradation.
活性药物成分、药物代谢物、候选药物的合成和分析,以及生物合成途径的研究、细菌多糖降解机制和天然物质(部分在复杂基质中)的结构澄清,是Greifswald大学药学院参与工作组的研究工作重点。上述所有问题都需要高分辨率准确的质量测量数据,这些数据以前可以使用研究所运行的混合质谱仪系统收集。该系统在使用13年后不再运作,将在平等的基础上予以取代。由于性能显著提高的液相色谱系统(超高性能液相色谱)已经越来越多地建立起来,因此这次将采购这样一种混合系统。使用所要求的设备确定分析物精确质量的能力得到了内部两个单四极杆系统和三重四极杆质谱仪的补充,该质谱仪于2020年购买,用于敏感定量,但无法以必要的精度测量高分辨率准确的质量数据。上述工作一方面补充了用于研究生物活性和病理生理刺激的细胞分析以及海洋生物技术领域的其他蛋白质组学项目。此外,该研究所还处理有关药物在生物体和模型系统中的运输,药物形式中活性成分的释放以及人类致病菌的耐药机制和毒力因素的问题,迄今为止尚未使用高分辨率准确的质量数据,但通过精确的质量测量可以获得额外的见解。所要求的设备配置旨在维护和丰富整个药学院的研究机会。研究目的是阐明钾通道调节剂的作用和副作用的分子和超分子基础,sirtuin抑制剂的鉴定和定量,药物代谢产物的研究和制备纯化,人工酶的产品混合物,二肽和三肽转运体的“转运载体”的表征,非洲药用植物提取物成分的结构表征,合成产物的表征,以发现新的原aspase-3激活剂,鉴定人类免疫细胞中未知的脂质介质和来自白色念珠菌的新氧脂质,共生海洋生物的蛋白质基因组学和澄清细菌多糖降解的未知机制。

项目成果

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其他文献

Internet-administered, low-intensity cognitive behavioral therapy for parents of children treated for cancer: A feasibility trial (ENGAGE).
针对癌症儿童父母的互联网管理、低强度认知行为疗法:可行性试验 (ENGAGE)。
  • DOI:
    10.1002/cam4.5377
  • 发表时间:
    2023-03
  • 期刊:
  • 影响因子:
    4
  • 作者:
  • 通讯作者:
Differences in child and adolescent exposure to unhealthy food and beverage advertising on television in a self-regulatory environment.
在自我监管的环境中,儿童和青少年在电视上接触不健康食品和饮料广告的情况存在差异。
  • DOI:
    10.1186/s12889-023-15027-w
  • 发表时间:
    2023-03-23
  • 期刊:
  • 影响因子:
    4.5
  • 作者:
  • 通讯作者:
The association between rheumatoid arthritis and reduced estimated cardiorespiratory fitness is mediated by physical symptoms and negative emotions: a cross-sectional study.
类风湿性关节炎与估计心肺健康降低之间的关联是由身体症状和负面情绪介导的:一项横断面研究。
  • DOI:
    10.1007/s10067-023-06584-x
  • 发表时间:
    2023-07
  • 期刊:
  • 影响因子:
    3.4
  • 作者:
  • 通讯作者:
ElasticBLAST: accelerating sequence search via cloud computing.
ElasticBLAST:通过云计算加速序列搜索。
  • DOI:
    10.1186/s12859-023-05245-9
  • 发表时间:
    2023-03-26
  • 期刊:
  • 影响因子:
    3
  • 作者:
  • 通讯作者:
Amplified EQCM-D detection of extracellular vesicles using 2D gold nanostructured arrays fabricated by block copolymer self-assembly.
使用通过嵌段共聚物自组装制造的 2D 金纳米结构阵列放大 EQCM-D 检测细胞外囊泡。
  • DOI:
    10.1039/d2nh00424k
  • 发表时间:
    2023-03-27
  • 期刊:
  • 影响因子:
    9.7
  • 作者:
  • 通讯作者:

的其他文献

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{{ truncateString('', 18)}}的其他基金

An implantable biosensor microsystem for real-time measurement of circulating biomarkers
用于实时测量循环生物标志物的植入式生物传感器微系统
  • 批准号:
    2901954
  • 财政年份:
    2028
  • 资助金额:
    --
  • 项目类别:
    Studentship
Exploiting the polysaccharide breakdown capacity of the human gut microbiome to develop environmentally sustainable dishwashing solutions
利用人类肠道微生物群的多糖分解能力来开发环境可持续的洗碗解决方案
  • 批准号:
    2896097
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
A Robot that Swims Through Granular Materials
可以在颗粒材料中游动的机器人
  • 批准号:
    2780268
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Likelihood and impact of severe space weather events on the resilience of nuclear power and safeguards monitoring.
严重空间天气事件对核电和保障监督的恢复力的可能性和影响。
  • 批准号:
    2908918
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Proton, alpha and gamma irradiation assisted stress corrosion cracking: understanding the fuel-stainless steel interface
质子、α 和 γ 辐照辅助应力腐蚀开裂:了解燃料-不锈钢界面
  • 批准号:
    2908693
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Field Assisted Sintering of Nuclear Fuel Simulants
核燃料模拟物的现场辅助烧结
  • 批准号:
    2908917
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Assessment of new fatigue capable titanium alloys for aerospace applications
评估用于航空航天应用的新型抗疲劳钛合金
  • 批准号:
    2879438
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
CDT year 1 so TBC in Oct 2024
CDT 第 1 年,预计 2024 年 10 月
  • 批准号:
    2879865
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Developing a 3D printed skin model using a Dextran - Collagen hydrogel to analyse the cellular and epigenetic effects of interleukin-17 inhibitors in
使用右旋糖酐-胶原蛋白水凝胶开发 3D 打印皮肤模型,以分析白细胞介素 17 抑制剂的细胞和表观遗传效应
  • 批准号:
    2890513
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship
Understanding the interplay between the gut microbiome, behavior and urbanisation in wild birds
了解野生鸟类肠道微生物组、行为和城市化之间的相互作用
  • 批准号:
    2876993
  • 财政年份:
    2027
  • 资助金额:
    --
  • 项目类别:
    Studentship

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气相色谱-高分辨率精确质谱仪
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合成和自然群落扩增子数据的准确和高分辨率分类
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