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Studies on Molecular Basis for Reactive Nitrogen Metabolism in Plants

植物活性氮代谢的分子基础研究

基本信息

批准号：
15570038
负责人：
SAKAMOTO Atsushi
金额：
$ 2.24万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

(1)Arabidopsis 2-Cys Peroxiredoxin as Metabolic Enzyme for Reactive Nitrogen SpeciesPurified recombinant proteins of a thiol-dependent peroxidase called 2-Cys peroxiredoxin (2CPRX) from Arabidopsis were shown to metabolically scavenge peroxynitrite, a reactive nitrogen species (RNS) known as a potent oxidizing and nitrating agent. The Arabidopsis 2CPRX cDNA was functionally able to complement the hypersensitivity of a yeast mutant to nitrite-derived RNS. These results demonstrated a new role of plant 2CPRX as a critical determinant of the resistance to RNS, and support the existence of a plant enzymatic basis for RNS metabolism.(2)Functional relevance of Arabidopsis Non-Symbiotic Hemoglobin to Reactive Nitrogen SpeciesPlants ubiquitously contain non-symbiotic hemoglobins whose physiological role remains obscure. Purified recombinant proteins of an Arabidopsis non-symbiotic hemoglobin (AtGLB1) were shown to possess peroxidase-like activity that can oxidize nitrite as electron donor to f … More orm nitrogen dioxide radicals. AtGLB1 mRNA significantly accumulated in Arabidopsis seedlings that had been exposed to nitrite. These results support the physiological relevance of the function of AtGLB1 to nitrite and nitrite-derived RNS, and suggest a new possible route for RNS production in plants, apart from nitric-oxide (NO) biogenesis.(3)Plant-Physiological Impact of Molecular-Genetic Alteration in S-Nitrosothiol MetabolismS-Nitrosothiols (RSNO), a major form of RNS, are NO-adducts of thiol compounds such as S-nitrosoglutathione (GSNO) that is a representative RSNO with biological relevance. Overexpression of Arabidopsis GSNO reductase (GSNOR), the only known plant enzyme possibly involved in RSNO metabolism, led to a significant RSNO reduction in transgenic Arabidopsis. In contrast, knocking out the GSNOR gene resulted in RSNO over-accumulation. Metabolic studies of NO_2 assimilation in transgenic Arabidopsis overexpressing GSNOR suggested the existence of a crosstalk between RNS metabolism and the primary nitrogen assimilation. These results strongly indicate GSNOR as a key enzyme in RSNO metabolism and its potential importance in plant nitrogen metabolism. Less

(1)拟南芥2-Cys过氧化物还蛋白作为活性氮的代谢酶从拟南芥中纯化的巯基依赖性过氧化物酶2-Cys过氧化物还蛋白（2CPRX）重组蛋白被证明可以代谢清除过氧亚硝酸盐，过氧亚硝酸盐是一种活性氮（RNS），被称为有效的氧化和硝化剂。拟南芥2CPRX cDNA在功能上能够补充酵母突变体对亚硝酸盐衍生RNS的超敏性。这些结果证明了植物2CPRX作为RNS抗性的关键决定因素的新作用，并支持RNS代谢存在植物酶基础。(2)拟南芥非共生血红蛋白与活性氮的功能相关性植物中普遍含有非共生血红蛋白，其生理作用尚不清楚。纯化的拟南芥非共生血红蛋白（AtGLB1）重组蛋白具有过氧化物酶样活性，可以氧化亚硝酸盐作为电子供体，以形成更多的二氧化氮自由基。暴露于亚硝酸盐的拟南芥幼苗中，AtGLB1 mRNA显著积累。这些结果支持了AtGLB1与亚硝酸盐和亚硝酸盐衍生RNS功能的生理相关性，并提出了除一氧化氮（NO）生物发生外植物RNS产生的新途径。s -亚硝基硫醇（RSNO）是硫醇类化合物的no加合物，如s -亚硝基谷胱甘肽（GSNO），是具有生物学相关性的代表性RSNO，是RNS的主要形式。转基因拟南芥GSNO还原酶（GSNOR）是唯一已知可能参与RSNO代谢的植物酶，其过表达导致转基因拟南芥中RSNO的显著减少。相反，敲除GSNOR基因导致RSNO过度积累。过表达GSNOR转基因拟南芥NO_2同化的代谢研究表明，RNS代谢与初级氮素同化之间存在串扰。这些结果强烈表明GSNOR是RSNO代谢的关键酶，在植物氮代谢中具有潜在的重要作用。少

项目成果

期刊论文数量（94）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Morikawa et al.: "Formation of unidentified nitrogen in plants : an implication for a novel nitrogen metabolism"Planta. (印刷中). (2004)

Morikawa 等人：“植物中未知氮的形成：对新型氮代谢的影响”Planta（出版中）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration

DOI：
10.1016/j.febslet.2004.07.005
发表时间：
2004-08-13
期刊：
FEBS LETTERS
影响因子：
3.5
作者：
Sakamoto, A;Sakurao, S;Morikawa, H
通讯作者：
Morikawa, H

Uptake, assimilation and novel metabolism of nitrogen dioxide in plants

植物对二氧化氮的吸收、同化和新代谢

DOI：
发表时间：
2007
期刊：
Phytoremediation : Methods and Reviews(Methods in Biotechnology Volume 23 : Neil Willey, ed.)(Humana Press, Totowa, NJ, USA)
影响因子：
0
作者：
Misa Takahashi;Toshiyuki Matsubara;Atsushi Sakamoto and Hiromichi Morikawa
通讯作者：
Atsushi Sakamoto and Hiromichi Morikawa

Phytoremediators from abandoned rice field.

来自废弃稻田的植物修复剂。

DOI：
发表时间：
2005
期刊：
Plant Biotechnology 22
影响因子：
0
作者：
Takahashi et al.;Morikawa et al.;Morikawa et al.;Kurumata et al.;Takahashi et al.
通讯作者：
Takahashi et al.

活性窒素代謝 -植物窒素代謝の新しい課題-

活性氮代谢-植物氮代谢的新挑战-