Regulation of plant metabolic function mediated by protein S-nitrosylation

蛋白质S-亚硝基化介导的植物代谢功能调节

• 批准号: 17570039
• 负责人: SAKAMOTO Atsushi
• 金额: $ 2.24万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17570039/
• 关键词: Protein S-nitrosylation; Reactive nitrogen species (RNS); S-nitrosothiol; S-nitrosoglutathione (GSNO); GSNO reductase; Nitrate assimilation; NO signaling; Arabidopsis; S-ニトロソグルタチオン; S-ニトロソ化合物; 無機窒素同化; アラビドプシス

S-Nitrosylation of cysteine residues is a critical redox modification that regulates a broad spectrum of protein functions, and this process involves reactive nitrogen species (RNS) such as S-nitrosothiols (RSNO) that are known to modify the thiol moiety of proteins. This research attempted to examine: (1) the importance of RSNO metabolism in modulating protein S-nitrosylation in higher plants, with special focus on the regulatory role of S-nitrosoglutathione reductase (GSNOR) as a key enzyme determining cellular RSNO levels and the degree of S-nitrosylation; and (2) the possibility that this posttranslational modification may participate in the regulation of certain plant metabolic functions such as nitrogen assimilation.(1) When exposed to RNS such as NO_2, NO donors and nitrate, Arabidopsis plants increased the level of RSNO, a substantial proportion of which was recovered in high-molecular fractions. This suggests the proteinous nature of accumulated RSNO in response to these inorg … More anic nitrogen oxides. Using biotin-switch method, the changes in S-nitrosylated protein levels were monitored and it was found that NO-donor treatments enhanced protein S-nitrosylation in wild-type plants. Overexpression of GSNOR, however, alleviated the extent of this posttranslational modification, with the increased level much lower than that seen in wild-type plants, supporting the importance of this enzyme in modulating protein S-nitrosylation.(2) The physiological consequence of GSNOR overexpression was investigated with respect to the regulatory aspects of nitrogen assimilation. When fed with a single ^<15>N-labeled nitrogen source such as nitrate or NO_2, transgenic plants overexpressing GSNOR incorporated and assimilated more nitrate-and NO_2-derived ^<15>N than did the wild-type plants. Because GSNOR is catalytically not related to the assimilatory process of inroganic nitrogen, these results suggest the possible involvement of GSNOR, and hence S-nitrosylation of proteins, in the regulation of nitrogen assimilation. Less

半胱氨酸残基的s -亚硝基化是一种关键的氧化还原修饰，可调节多种蛋白质功能，这一过程涉及活性氮物质（RNS），如s -亚硝基硫醇（RSNO），已知其可修饰蛋白质的硫醇部分。本研究试图探讨：(1)RSNO代谢在高等植物蛋白s -亚硝基化中的重要作用，重点研究了s -亚硝基谷胱甘肽还原酶（S-nitrosoglutathione reductase， GSNOR）作为决定细胞RSNO水平和s -亚硝基化程度的关键酶的调控作用；(2)这种翻译后修饰可能参与调控某些植物代谢功能，如氮同化。(1)当暴露于NO_2、NO供体和硝酸盐等RNS时，拟南芥植株的RSNO水平升高，其中很大一部分以高分子组分的形式恢复。这表明积累的RSNO的蛋白质性质是对这些无机氮氧化物的反应。利用生物素开关法监测了s -亚硝基化蛋白水平的变化，发现no供体处理增强了野生型植物s -亚硝基化蛋白水平。然而，GSNOR的过表达减轻了这种翻译后修饰的程度，其增加的水平远低于野生型植物，支持该酶在调节蛋白质s -亚硝基化中的重要性。(2)研究了GSNOR过表达在氮同化调控方面的生理后果。当以单一的^<15>N标记的氮源（如硝酸盐或NO_2）喂养时，过度表达GSNOR的转基因植物比野生型植物吸收和同化了更多的硝酸盐和NO_2衍生的^<15>N。由于GSNOR在催化上与无机氮的同化过程无关，这些结果表明GSNOR可能参与氮同化的调节，因此蛋白质的s -亚硝基化。少

项目成果

Uptake, assimilation and novel metabolism of nitrogen dioxide in plants

植物对二氧化氮的吸收、同化和新代谢

• 发表时间: 2007
• 期刊: Phytoremediation : Methods and Reviews(Methods in Biotechnology Volume 23 : Neil Willey, ed.)(Humana Press, Totowa, NJ, USA)
• 作者: Misa Takahashi;Toshiyuki Matsubara;Atsushi Sakamoto and Hiromichi Morikawa
• 通讯作者: Atsushi Sakamoto and Hiromichi Morikawa

Reactive nitrogen metabolism : a novel frontier in plant nitrogen metabolism.

活性氮代谢：植物氮代谢的新前沿。

• 发表时间: 2005
• 期刊: Plant Biotechnology 22
• 作者: J.Shigeto et al.;J.Shigeto et al.;Morikawa et al.;J.Shigeto et al.;Morikawa et al.;M.Kurumata et al.;M.Takahashi et al.;H.Hokazono et al.;M.Takahashi et al.;M.Takahashi et al.;Morikawa et al.;Takahashi et al.;Hokazono et al.;M.Takahashi et al.;A.Sakamoto et al.
• 通讯作者: A.Sakamoto et al.

TJ1 is an orientation-independent transformation enhancer sequence

TJ1 是方向无关的转化增强子序列

• 发表时间: 2005
• 期刊: Plant Biotechnol 22
• 作者: H.Hokazono;M.Takahashi;A.Sakamoto;H.Morikawa
• 通讯作者: H.Morikawa

Phytoremediators from abandoned rice field.

来自废弃稻田的植物修复剂。

• 发表时间: 2005
• 期刊: Plant Biotechnology 22
• 作者: Takahashi et al.;Morikawa et al.;Morikawa et al.;Kurumata et al.;Takahashi et al.
• 通讯作者: Takahashi et al.

Focus on Plant Molecular Biology-2 : Biotechnological Approaches to Improve Nitrogen Use Efficiency in Plants (R.P. Singh and P.K. Jaiwal, eds.)

聚焦植物分子生物学-2：提高植物氮利用效率的生物技术方法（R.P. Singh 和 P.K. Jaiwal 编辑）

• 发表时间: 2006
• 作者: J.Shigeto et al.;J.Shigeto et al.;Morikawa et al.;J.Shigeto et al.;Morikawa et al.;M.Kurumata et al.;M.Takahashi et al.;H.Hokazono et al.;M.Takahashi et al.;M.Takahashi et al.;Morikawa et al.;Takahashi et al.;Hokazono et al.;M.Takahashi et al.;A.Sakamoto et al.;A.Nakagawa et al.;A.Nakagawa et al.;M.Takahashi et al.;M.Takahashi et al.;M.Takahashi et al.(分担執筆);M.Takahashi et al.(分担執筆);Morikawa et al.(分担執筆);H.Morikawa et al.(分担執筆)
• 通讯作者: H.Morikawa et al.(分担執筆)