Analysis and proposal of new aluminum resistanece strategy by developing of model technique using artificial cell membrane

目标已基本完成，最终可以提出一种新的铝电阻策略。我承认对我的研究建议的采纳做出了极好的判断。本研究的主要内容如下：(1)建立了一种新的、简便的分离根尖质膜原生质体的方法。发现附着的残膜几乎不与其他细胞器膜分离。(2)合成包膜尼龙胶囊渗透性实验系统的建立。合成尼龙胶囊包膜尼龙-2，合成了8粒胶囊，并在其膜的微孔表面包覆了脂质双层。该实验系统优于传统的脂质体系统。根中有机酸阴离子释放与…无关抗铝小黑麦根尖原生质体的亚甲蓝(MB)染色能力与抗铝小黑麦根尖原生质体的亚甲基蓝(MB)染色能力呈显著负相关。豌豆油菜素类固醇(BR)突变体和赤霉素(GA)突变体的抗铝性比较。在几个豌豆BR突变体和GA突变体中，Ih突变体对高铝条件最为敏感。这种最高的敏感性与PM的最大透性相关，而与根中的OA释放无关。这可能与质膜脂层中游离甾醇的异常积累有关。(5)信号草的超铝抗性根尖质膜对黑麦草的渗透性相当低。新发现黄酮类化合物在根尖细胞周围高度沉积；这也有助于黑麦草PM的最低渗透性较少

The objectives have almost been completed, and finally a new aluminum resistance strategy could be proposed. I acknowledge the excellent judgement on the adoption of my research proposal. The points of my research are as follows.(1)Development of new and simple technique for isolation of plasma mebrane(PM)Protoplasts isolated from root-tips were adhered on polylysine coated glass plates, and washed with buffer solution. The adhered residual membranes were found to be almost free from other organelle membranes. Finally, I could develop a new simple technique for isolation of PM as compared with conventional liquid two polymer phase partitioning technique.(2)Establishment of permeability experiment system using synthesized nylon capsule coated with PM lipidNylon-2, 8 capsules were synthesized and the micropores in their membranes were coated with lipid bilayer. This experimental system was superior to the converntional liposome system. Organic acid anion release from roots was not signif … More icantly different between two lines of triticale, but on the other hand, PM lipid which is qualitatively rich in free sterols and glucocerebrosides of Al-resistant triticale line was less permeable than that of Al-sensitive line in Al medium.(3)Association of methylene blue(MB)stainability of protoplasts isolated from root-tips with Al resistanceSignificant negative correlation was recognized between Al resistance and MB stainability of protoplasts among many plant species, cultivars and lines. This suggests that Al resistant plants have their root-tips with lower negativity and stronger PM.(4)Comparison of Al resistances among brassinosteroid(BR) mutants and gibberellin (GA) mutants of peaAmong several BR mutants and GA mutants of pea, Ih mutant was most sensitive to high Al conditions. This highest sensitivity was correlated with the greatest permeability of PM, but not with OA release from roots. This may be associated with the abnormal accumulation of free sterols in PM lipid layer.(5)Hyper Al resistance of signalgrassPermeability of root-tip PM for signalgrass was considerably low. High deposition of flavonoids around the peripheral area of root-tip cells was newly found ; this can also contribute to the least permeability of signalgrass PM. Less