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Study on application of cyanobacterial vector

Study on application of cyanobacterial vector
蓝藻载体的应用研究
批准号:
15580168
负责人:
MAEDA Hiroto
金额:
$2.43万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2006

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中文摘要
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英文摘要
Autotrophic picoplankton are a group of microorganisms that affect biogeochemical processes and influence the fate of primary production in oceans and lakes. To understand the ecological role of cyanophage and its effect on blooming of cyanobacteria and nutrients cycling in the coastal marine environment, it is critical to understand cyanophage infectivity and the interactions that occur between the phage and its host marine cyanobacterial picoplankton. In this study, we examined to apply of cyanophage for a vector as a tool of molecular biotechnology.We isolated a new virulent cyanophage (strain S-KM1), and here have investigated its seasonal infectivity of cyanophage, phycoerythrin-rich Synechococcus sp. (strain KFM001) in Kagoshina Bay. We found that the phage titers ranged from undetectable to 750000 cells/ml in autumn, and began to increase about one week after the onset of the Synechococcus autumn bloom. During the autumn months, the phage numbers oscillated in a fashion that minored levels of host abundance, showing a lag of about one week. We calculated that from 0. 0055% to 2. 1% of the host population per day was lysed by the phage during a Synechococcus bloom in autumn. This suggests that lyic phages have a noticeable efect on regulating the densities of marine Svnechococcus populations.Also, we showed their transmission electron micrography of negative-stained and the characteristics of phage DNA and molecular phylogenetic relationship between our phage and others. As a tool of molecular biotechnology, we examined to application of those cyanophage for a vector. But, it is not succeed for the reason that the phage have a very narrow host range and not so strong infectivity. The phage do not have an tolerant capacity to preservation. It was found throug this study that wide host range, strong infectivity and tolerant capacity to preservation were important to application of those cyanophage for a vector.
期刊论文(33)
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会议论文
紫外線による赤潮駆除に関する研究
紫外线消除赤潮的研究
DOI: --
发表时间: 2006
期刊: 日本海水学会誌 60
影响因子: --
作者: [前田広人, 池田俊之, 田岡洋介, 奥西将之, 日高正康, Crispino Saclauso]
通讯作者: Crispino Saclauso
汚損生物駆除素剤
污垢生物剂
DOI: --
发表时间: 2006
期刊:
影响因子: --
作者: []
通讯作者:
DOI: --
发表时间: 2006
期刊: 防菌防黴 34
影响因子: --
作者: [塩崎靖志, 前田広人, 田岡洋介, 越塩俊介, 吉川毅, 坂田泰造]
通讯作者: 坂田泰造
DOI: --
发表时间: 2006
期刊: Bull. Soc. Sea Water Sci., Jpn. 60
影响因子: --
作者: [IMADA o., H.MAEDA, Y.TANAKA]
通讯作者: Y.TANAKA
20
    Determination of mechanism of red tide extinction and suppression of cyst germination by reagents
    • 批准号:
      23580258
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
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    • 财政年份:
      2011
    • 负责人:
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    Analysis of bacterial populationin dynamics of sediment in aqaculture area by use of flowcytometry and sediment remediation
    • 批准号:
      12660177
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.98万
    • 财政年份:
      2000
    • 负责人:
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    国内基金
    海外基金
    phage display联合基因转染技术对Bcl-2蛋白质结构和功能的研究
    • 批准号:
      30471999
    • 项目类别:
      面上项目
    • 资助金额:
      8.0万元
    • 批准年份:
      2004
    • 负责人:
      孙志扬
    • 依托单位: