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Study on application of cyanobacterial vector

蓝藻载体的应用研究

基本信息

批准号：
15580168
负责人：
MAEDA Hiroto
金额：
$ 2.43万
依托单位：
Mie University (2005-2006)Kagoshima University (2003-2004)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

项目摘要

Autotrophic picoplankton are a group of microorganisms that affect biogeochemical processes and influence the fate of primary production in oceans and lakes. To understand the ecological role of cyanophage and its effect on blooming of cyanobacteria and nutrients cycling in the coastal marine environment, it is critical to understand cyanophage infectivity and the interactions that occur between the phage and its host marine cyanobacterial picoplankton. In this study, we examined to apply of cyanophage for a vector as a tool of molecular biotechnology.We isolated a new virulent cyanophage (strain S-KM1), and here have investigated its seasonal infectivity of cyanophage, phycoerythrin-rich Synechococcus sp. (strain KFM001) in Kagoshina Bay. We found that the phage titers ranged from undetectable to 750000 cells/ml in autumn, and began to increase about one week after the onset of the Synechococcus autumn bloom. During the autumn months, the phage numbers oscillated in a fashion that minored levels of host abundance, showing a lag of about one week. We calculated that from 0. 0055% to 2. 1% of the host population per day was lysed by the phage during a Synechococcus bloom in autumn. This suggests that lyic phages have a noticeable efect on regulating the densities of marine Svnechococcus populations.Also, we showed their transmission electron micrography of negative-stained and the characteristics of phage DNA and molecular phylogenetic relationship between our phage and others. As a tool of molecular biotechnology, we examined to application of those cyanophage for a vector. But, it is not succeed for the reason that the phage have a very narrow host range and not so strong infectivity. The phage do not have an tolerant capacity to preservation. It was found throug this study that wide host range, strong infectivity and tolerant capacity to preservation were important to application of those cyanophage for a vector.

自养微微浮游生物是一组影响生物地球化学过程和影响海洋和湖泊初级生产力命运的微生物。为了了解噬藻体的生态作用及其对沿海海洋环境中蓝藻水华和营养物质循环的影响，了解噬藻体的感染性以及噬菌体与宿主海洋蓝藻微浮游动物之间的相互作用是至关重要的。本研究探索了噬藻体作为载体作为分子生物技术工具的应用，分离到一株新的强毒噬藻体S-KM1，并对其对富含藻红蛋白的聚球藻球藻的季节性感染性进行了研究。(菌株KFM001)。我们发现，秋季噬菌体滴度从检测不到到750000个/毫升，并在聚球藻秋季开花后约一周开始增加。在秋季的几个月里，噬菌体的数量以一种降低宿主丰度水平的方式振荡，表现出大约一周的滞后。我们从0开始计算。在秋季聚球藻开花期间，每天有0055%至2.1%的寄主种群被噬菌体裂解。结果表明，裂解噬菌体在调节海洋链球菌种群密度方面具有显著作用，并展示了其负染的透射电子显微镜、噬菌体DNA特征及与其他噬菌体的分子系统发育关系。作为分子生物技术的工具，我们研究了这些噬藻体在载体中的应用。但是，由于噬菌体的寄主范围很窄，感染性不强，所以没有成功。噬菌体不具有耐受保存的能力。通过本研究发现，寄主范围广、感染力强、耐保存能力强是这些噬藻体作为媒介应用的重要条件。