Analysis of bacterial populationin dynamics of sediment in aqaculture area by use of flowcytometry and sediment remediation

本研究的目的是将流式细胞术和荧光光度法等光学技术应用于海底沉积物细菌动态分析和沉积物质量修复。本研究进行了室内试验和现场调查。在实验室实验中，为了了解大肠杆菌作为细菌模型的环境适应性，我们开始使用荧光抗体方法，从而可以测量sigma蛋白，明确细菌的环境适应性机制，并为该方法应用于细菌分析奠定了前景。由于我们必须制作每个细菌抗体才能用流式细胞仪对细菌进行计数，因此我们利用流式细胞仪对微浮游生物作为细菌模型进行原位调查鉴定作为开始。为了探讨浮游生物的种群数量及其在环境中的行为，采用直接计数法和流式细胞术根据色素类型的区分进行计数。鹿儿岛湾的水质在过去几年中基本保持稳定，但富营养化并没有停止，这从赤潮的出现和溶解氧的耗尽就可以看出。本研究的重点是底部溶解氧的消耗，这是在海湾的内部关闭过于关键。数据表明鹿儿岛湾富营养化程度已达到临界水平，沉积物状况需要进行修复。

The purpose of this study is to apply optical technique such as flow cytometry and fluorescence photometery to analysis of dynamics of bacteria in bottom sediment and remediation of sediment quality. For this research, laboratry experiment and in situ investigation were done as follows.In laboratry experiment, we started to use fluorescence antibody method in order to know the environmental adaptation of Escherchia coli as a model of bacteria As a result, sigma protein could be measured and the mechanism of environment adaptation the bacteria could be made clear, and the prospect of application of this methods apply to bacterial analysis could be established.Since we must make each bacterial antibody in order to count of bacteria by direct flow cytometery, we utilized flow cytometer to in situ investigation for discrmination of picoplankton as a model of bacteria as the beginning. To probe to quantify of the in situ picoplankton population and their behavior in environments, we examined to enumeration by direct counting and flow cytometry according to discrimination between types of pigments.The water quality of Kagoshima bay has remained more or less stable during the last several years, but eutrophication has by no means been halted, as shown by the appearance of red tide and depletion of dissolved oxygen. This research focuses on the depletion of dissolved oxygen in the bottom, which is brought to close too critical in the inner part of the bay. The data indicated that the degree of eutrophication in Kagoshima Bay has been critical and the sediment condition suggusted to need a remediation.