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Analysis of bacterial populationin dynamics of sediment in aqaculture area by use of flowcytometry and sediment remediation

流式细胞术和沉积物修复分析水产养殖区沉积物细菌种群动态

基本信息

批准号：
12660177
负责人：
MAEDA Hiroto
金额：
$ 1.98万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The purpose of this study is to apply optical technique such as flow cytometry and fluorescence photometery to analysis of dynamics of bacteria in bottom sediment and remediation of sediment quality. For this research, laboratry experiment and in situ investigation were done as follows.In laboratry experiment, we started to use fluorescence antibody method in order to know the environmental adaptation of Escherchia coli as a model of bacteria As a result, sigma protein could be measured and the mechanism of environment adaptation the bacteria could be made clear, and the prospect of application of this methods apply to bacterial analysis could be established.Since we must make each bacterial antibody in order to count of bacteria by direct flow cytometery, we utilized flow cytometer to in situ investigation for discrmination of picoplankton as a model of bacteria as the beginning. To probe to quantify of the in situ picoplankton population and their behavior in environments, we examined to enumeration by direct counting and flow cytometry according to discrimination between types of pigments.The water quality of Kagoshima bay has remained more or less stable during the last several years, but eutrophication has by no means been halted, as shown by the appearance of red tide and depletion of dissolved oxygen. This research focuses on the depletion of dissolved oxygen in the bottom, which is brought to close too critical in the inner part of the bay. The data indicated that the degree of eutrophication in Kagoshima Bay has been critical and the sediment condition suggusted to need a remediation.

本研究旨在应用流式细胞仪和荧光光度计等光学技术分析底泥细菌动态及底泥质量修复。本研究主要进行了以下几个方面的实验室研究和现场调查：实验室研究采用荧光抗体法，以了解土栖大肠杆菌作为模式菌的环境适应性，通过对土栖大肠杆菌的sigma蛋白的测定，明确土栖大肠杆菌的环境适应机制，由于采用直接流式细胞仪进行细菌计数必须制备每种细菌的抗体，以微微型浮游生物作为细菌的模式生物，利用流式细胞仪进行了现场调查。为探讨微型浮游生物的数量及其在环境中的行为，采用直接计数法和流式细胞仪对微型浮游生物进行了计数。鹿儿岛湾的水质在过去几年中基本保持稳定，但富营养化并未停止，赤潮的出现和溶解氧的减少表明了这一点。这项研究的重点是溶解氧的底部，这是关闭太关键的海湾内部的耗尽。研究结果表明，鹿儿岛湾的富营养化程度已达到临界值，沉积物状况已恶化，需要进行修复。