Experimentally induced Vogt-Koyanagi-Harada disease in two Akita dogs

我们研究了用酪氨酸酶相关蛋白1 （TRP1）免疫秋田犬是否会诱发Vogt-Koyanagi-Harada （VKH）样疾病，并将其与秋田犬自发发生的类似人类VKH疾病的疾病诱导的改变进行了比较。两只秋田犬用人类TRP1肽混合物免疫。观察眼内变化，分别行裂隙灯生物显微镜、眼镜和荧光素血管造影（FA）检查。在第一次免疫后约20个月的一只狗（狗1）和第二次免疫后3周的另一只狗（狗2）用标准组织学方法研究了眼睛、皮肤和大脑。在第一次免疫接种后3-4周，两只狗都出现了绒毛膜视网膜疾病。许多炎性细胞浸润到前房和前玻璃体。眼底表现为地域性、多灶性渗出性视网膜脱离。从脉络膜处检测到荧光素多灶性渗漏。组织学上，第二次免疫后3周，2号犬眼睛视网膜下间隙可见炎性细胞。部分病变处炎性细胞浸润使脉络膜增厚。犬2眼可见Dalen-Fuchs结节。刺激后3周皮肤中可见毛囊和血管周围的色素沉着、色素分散和许多炎症细胞浸润。眼睛和皮肤的临床过程和变化与自发发生VKH疾病的秋田犬非常相似。我们得出结论，在这些犬中已经诱导了VKH样疾病，这支持了秋田犬自发发生的绒毛膜视网膜疾病是VKH病的初步结论。

We have investigated whether a Vogt-Koyanagi-Harada (VKH)-like disease can be induced in Akita dogs by immunizing them with tyrosinase related protein 1 (TRP1), and compared the alterations induced to those of Akita dogs with a spontaneously occurring disease that resembles human VKH disease. Two Akita dogs were immunized with a peptide mixture of human TRP1. The changes in the eyes were followed by slit-lamp biomicroscopy, ophthalmoscopy, and fluorescein angiography (FA). The eyes, skin, and brains were studied by standard histological methods at about 20 months after the first immunization in one dog (dog 1), and at 3 weeks after the second immunization in the second dog (dog 2). Both dogs developed chorioretinal disease 3-4 weeks after the first immunization. Many inflammatory cells infiltrated into the anterior chamber and anterior vitreous. The fundus showed geographic, multifocal exudative retinal detachments. Multifocal leakages of fluorescein were detected from the choroid. Histologically, exudative retinal detachment was present, and inflammatory cells were seen in the subretinal space in the eyes of dog 2 taken three weeks after the second immunization. The choroid was thickened by the infiltration of inflammatory cells in some lesions. Dalen-Fuchs nodules were seen in the eye of dog 2. Depigmentation, pigment dispersion, and infiltration of many inflammatory cells around hair follicles and vessels were seen in the skin taken three weeks postimmunization. The clinical course and changes in the eyes and skin were very similar to those seen in the Akita dogs with spontaneously occurring VKH disease. We concluded that a VKH-like disease had been induced in these dogs, and this supports the tentative conclusion that the spontaneously occurring chorioretinal disease in Akita dogs is VKH disease.