Design and synthesis of hybrid-type molecular probes useful for elucidation of molecular mechanisms of physiological events caused by brassinosteroids

杂合型分子探针的设计和合成有助于阐明油菜素类固醇引起的生理事件的分子机制

基本信息

批准号：
15590034
负责人：
SETO Hideharu
金额：
$ 2.05万
依托单位：
RIKEN
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Brassinosteroids(BRs) constitute a class of steroidal plant hormones, which are essential for plant growth and development. Many efforts have been made in a wide range of research fields in order to elucidate the real features of BR actions at molecular level in plants, using technologies based on molecular biology. In this project, I aimed the design and synthesis of BR-based hybrid-type molecular probes useful for elucidation of molecular mechanisms of physiological events caused by BRs. and made considerable achievements as follows.1.Starting with natural BRs, brassinolide(BL) and castasterone(CS), the synthesis of several BR-based hybrid-type molecular probes : i.e., phenyldiazirine/biotin double-labelled BR, biotin labeled BR and dexamethazone labeled BR, were completed.2.In early works on BR perception mechanism in plant, we elucidated that a transmembrane receptor kinase identified from Arabidopsis, BRI1,is a receptor of BRs. The photoaffinity labeling experiments using aryldiazirine/biotin double-labelled CS synthesized as a photoaffinity probe clarified that BR directly binds to BRI1 without other proteins or peptides. Furthermore, the minimal binding domain for BR consisting of 94 amino acids was identified, which was a new binding domain for steroid hormones.3.26-hydroxy-CS and -BL synthesized in this project as synthetic intermediates to BR-based hybrid-type molecular probes played a crucial role in the functional analysis of the Arabidopsis CYP72B1,BAS1 and SOB7 genes. It was elucidated that these genes inactivated BRs through 26-hydroxylation of active BRs, CS and BL.4.In connection with this project, four positional isomers of mono-O-β-D-glucopyranosides of BL and CS with tetrahydroxy groups were prepared. These glycosides played a crucial role in the functional analysis of the Arabidopsis UGT73C5 gene expressing a UPD-glycosyltransferase, UGT73C5. It was elucidated that UGT73C5 specifically glycosylated 23-hydroxyl function of CS and BL.

黄铜固醇（BRS）构成了一类类固醇植物恐怖剂，这对于植物生长和发育至关重要。为了阐明基于分子生物学的技术，在植物分子水平上阐明了BR作用在分子水平上的真实特征，已经在广泛的研究领域中做出了许多努力。在这个项目中，我针对基于BR的杂交型分子问题的设计和合成，可用于阐明由BRS引起的物理事件的分子机制。 and made considerable achievements as follows.1.Starting with natural BRs, brassinolide(BL) and castasterone(CS), the synthesis of several BR-based hybrid-type molecular problems: i.e., phenyldiazirine/biotin double-labelled BR, biotin labeled BR and dexamethazone labeled BR, were completed.2.In early works on BR perception mechanism in plant, we阐明了从拟南芥BRI1鉴定的跨膜受体激酶是BR的受体。使用Aryldiazirine/Biotin双标记的CS合成为光附近探针的光亲和力标记实验阐明了BR直接与BRI1结合，而无需其他蛋白质或辣椒。此外，确定了由94个氨基酸组成的BR的最小结合结构域，这是类固醇激素的新结合结构域。3.26-羟基-CS和在该项目中合成的合成中综合为BR基于BR的Hybrid-type分子探针在该项目中合成的综合作用，在该项目中合成了均具有Aripy subcial subcial subcial subcials sob1的作用。基因。阐明了这些基因通过活性BR，CS和BL.4的26-羟基化灭活的BR.4。这些糖苷在表达UPD-糖基转移酶UGT73C5的拟南芥UGT73C5基因的功能分析中起着至关重要的作用。阐明了CS和BL的UGT73C5特异性糖基化的23-羟基功能。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

植物の生長ホルモン・ブラシノステロイドと受容体BRI1の結合メカニズム

植物生长激素油菜素内酯与受体BRI1的结合机制

DOI：
发表时间：
2005
期刊：
BRAIN Techno News 109(印刷中)
影响因子：
0
作者：
瀬戸秀春;木下俊則
通讯作者：
木下俊則

Brassinosteroids bind to the extracellular domainof plant receptor kinase BRI1.

油菜素类固醇与植物受体激酶 BRI1 的胞外域结合。

DOI：
发表时间：
2005
期刊：
Cell Technology (in Japanese) 433
影响因子：
0
作者：
T.Kinoshita;H.Seto
通讯作者：
H.Seto

CYP72B1 inactivates brassinosteroid hormones : intersection between photomorphogenesis and plant steroid signal transduction

CYP72B1 灭活油菜素类固醇激素：光形态发生与植物类固醇信号转导之间的交叉

DOI：
发表时间：
2003
期刊：
Plant Physiol. 133
影响因子：
0
作者：
T.Kinoshita;A.Cano-Delgado;H.Seto;S.Hiranuma;S.Fujioka;S.Yoshida;J.Chory;E.M.Turk et al.
通讯作者：
E.M.Turk et al.

CYP72B1 inactivates brassinosteroid hormones : intersection between photomorphogenesis and plant steroid signal transduction.

CYP72B1 使油菜素类固醇激素失活：光形态发生和植物类固醇信号转导之间的交叉。

DOI：
发表时间：
2003
期刊：
Plant Physiol. 133
影响因子：
0
作者：
T.Kinoshita;A.Cano-Delgado;H.Seto;S.Hiranuma;S.Fujioka;S.Yoshida;J.Chory;E.M.Turk et al.;E.M.Turk et al.
通讯作者：
E.M.Turk et al.

ブラシノステロイドは植物受容体キナーゼBRI1の細胞外ドメインに結合する

油菜素类固醇与植物受体激酶 BRI1 的胞外域结合