Exposure evaluation of environmental pollutants using temperature-sensitive polymer

温度敏感型聚合物具有提高极限临界溶解温度(LCST)以下的溶解度和降低其在水中的溶解度的特性。在本研究中，利用温敏聚合物的这一特性，我们尝试将温敏聚合物作为从粗蛋白混合物中选择性分离浓缩标记蛋白的材料。4种聚合物，N-异丙基丙烯酰胺聚合物(P(NIPAAm))(分子量10,000)，P(NIPAAM95%-co-BMA5%)(分子量5,000)，P(NIPAAM95%-co-BMA5%-DMAPAAm5%)(分子量5,000)，P(NIPAAM85%-co-BMA5%-co-DMAPAAm10%)(分子量5,000)和P(NIPAAm)(分子量5,000)，并比较了P(NIPAAM85%-co-BMA5%-co-DMAPAAM10%)(分子量5,000)和P(NIPAAM)(分子量5,000)的特异性结合能力。该聚合物由N，N-二甲基甲酰胺和甲基丙烯酸丁酯共聚而成。PNIPAAm的聚合物(摩尔质量分数为10,000)是结合能力和连接性最好的聚合物。我们检测了不利的e-…环境污染物对二甲基亚砜和粒细胞集落刺激因子诱导HL60细胞分化为中性粒细胞的影响更大。我们通过环境污染物暴露引起的标志蛋白CD18蛋白水平的升高或降低来评估其不良影响。细胞在分化开始后连续暴露4d，作用4d后细胞杀伤率达到20%左右。接触苯并[a]芘等几种化合物后，CD18蛋白含量下降。而在51种化合物中，有许多化合物暴露后CD18蛋白含量增加。在这些化合物中，有机磷农药氧基形式的化合物、内分泌干扰化学品和消毒副产物包括。由此可见，多种环境污染物促进了细胞分化过程中CD18蛋白的生物合成。因此，这表明这些化合物在分化过程中刺激了免疫反应的作用。较少

The temperature-sensitive polymer has the property that it increases the solubility under the limited-critical soluble temperature (LCST) and decreases the solubility upper LCST in water. In this study, using this property of the temperature-sensitive polymer, we tried that the temperature-sensitive polymer was utilized as a material of selective fractionation and concentration of the marker protein form crude protein mixture. Four types of the polymer, N-isopropylacrylamide polymer (P(NIPAAm)) (Mol.Wt.10,000), P(NIPAAm95% - co-BMA5%) (Mol.Wt.5,000), P(NIPAAm95%-co-BMA5%-DMAPAAm5%) (Mol.Wt.5,000), P(NIPAAm85%-co-BMA5%-co-DMAPAAm10%) (Mol.Wt.5,000) and P(NIPAAm) (Mol.Wt.5,000), were examined and compared the ability of specific binding to the marker protein. The polymer was produced by copolymerization of N, N-dimethylformamide and butylmethacrylate. The polymer of PNIPAAm (Mol.Wt.10,000) was the most excellent property in binding capacity and connectivity.We have examined the adverse e … More ffect of the environmental pollutants on HL60 cells that were induced to neutrophilic cells by dimethyl sulfoxide and G-CSF. We evaluated the adverse effect by the increase or decrease of the CD18 protein level of the marker protein induced by the exposure of the environmental pollutants. Cells were exposed continuously for 4 days from the initiation of differentiation at the concentration in which the cell lethality after 4days exposures became about 20%. The CD18 protein quantity deceased by the exposure of several compounds, benzo[a]pyrene etc.. However, the CD18 protein quantity increased by the exposure of many compounds among 51 compounds. Among these compounds, compounds in the groups of the oxon forms of organophosphorus pesticides, the endocrine disrupting chemicals and disinfection by-products were includes. From these results, many kinds of environmental pollutants increased the biosynthesis of CD18 protein in differentiation process. So, it indicates that these compounds stimulate the immune response action in the differentiation process. Less