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Exposure evaluation of environmental pollutants using temperature-sensitive polymer

利用温敏聚合物进行环境污染物暴露评价

基本信息

批准号：
15590119
负责人：
NISHIMURA Tetsuji
金额：
$ 1.98万
依托单位：
National Institute of Health Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

The temperature-sensitive polymer has the property that it increases the solubility under the limited-critical soluble temperature (LCST) and decreases the solubility upper LCST in water. In this study, using this property of the temperature-sensitive polymer, we tried that the temperature-sensitive polymer was utilized as a material of selective fractionation and concentration of the marker protein form crude protein mixture. Four types of the polymer, N-isopropylacrylamide polymer (P(NIPAAm)) (Mol.Wt.10,000), P(NIPAAm95% - co-BMA5%) (Mol.Wt.5,000), P(NIPAAm95%-co-BMA5%-DMAPAAm5%) (Mol.Wt.5,000), P(NIPAAm85%-co-BMA5%-co-DMAPAAm10%) (Mol.Wt.5,000) and P(NIPAAm) (Mol.Wt.5,000), were examined and compared the ability of specific binding to the marker protein. The polymer was produced by copolymerization of N, N-dimethylformamide and butylmethacrylate. The polymer of PNIPAAm (Mol.Wt.10,000) was the most excellent property in binding capacity and connectivity.We have examined the adverse e … More ffect of the environmental pollutants on HL60 cells that were induced to neutrophilic cells by dimethyl sulfoxide and G-CSF. We evaluated the adverse effect by the increase or decrease of the CD18 protein level of the marker protein induced by the exposure of the environmental pollutants. Cells were exposed continuously for 4 days from the initiation of differentiation at the concentration in which the cell lethality after 4days exposures became about 20%. The CD18 protein quantity deceased by the exposure of several compounds, benzo[a]pyrene etc.. However, the CD18 protein quantity increased by the exposure of many compounds among 51 compounds. Among these compounds, compounds in the groups of the oxon forms of organophosphorus pesticides, the endocrine disrupting chemicals and disinfection by-products were includes. From these results, many kinds of environmental pollutants increased the biosynthesis of CD18 protein in differentiation process. So, it indicates that these compounds stimulate the immune response action in the differentiation process. Less

温敏聚合物具有在极限临界溶解温度（LCST）以下增加溶解度和在LCST以上降低溶解度的性质。本研究利用温敏性高分子的这一特性，尝试将温敏性高分子作为从粗蛋白混合物中选择性分离和浓缩标记蛋白的材料。四种聚合物，N-异丙基丙烯酰胺聚合物（P（NIPAAm））（分子量10，000），P（NIPAAm95% -共-BMA5%）（分子量5，000），P（NIPAAm95%-共-BMA5%-DMAPAAm5%）检测P（NIPAAm）（摩尔重量5，000）、P（NIPAAm 85%-共-BMA 5%-共-DMAPAAm 10%）（摩尔重量5，000）和P（NIPAAm）（摩尔重量5，000），并比较特异性结合标记蛋白的能力。该聚合物由N，N-二甲基甲酰胺和甲基丙烯酸丁酯共聚而成。PNIPAAm（分子量10，000）的聚合物在结合能力和连接性方面是最优异的。 ...更多信息环境污染物对二甲基亚砜和G-CSF诱导HL 60细胞向嗜酸性细胞分化的影响。我们通过暴露于环境污染物引起的标志蛋白CD 18蛋白水平的升高或降低来评估不良反应。从分化开始，以暴露4天后细胞致死率变为约20%的浓度连续暴露细胞4天。苯并[a]芘等多种化合物的暴露均使CD 18蛋白含量降低。但在51种化合物中，有许多化合物的暴露使CD 18蛋白量增加。这些化合物包括有机磷农药的氧磷形式、内分泌干扰物和消毒副产物。由此可见，多种环境污染物可促进分化过程中CD 18蛋白的合成。因此，这表明这些化合物在分化过程中刺激免疫应答作用。少