Study on the role of mutated α-tubulin in the progression of malignant tumors

突变α-微管蛋白在恶性肿瘤进展中的作用研究

• 批准号: 15590296
• 负责人: TAKANO Yasuo
• 金额: $ 2.3万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590296/
• 关键词: α-tubulin; Malignant tumor; Prognosis; 予後

The subjects to perform the object of the present study were : 1)Inspection of overexpression of mutated α-tubulin recognized by Y-49 in the broad spectrum of malignant tumor. 2)Analysis of biological characteristics of malignant tumors overexpressing mutated α-tubulin. 3)Detection of other biological partners in cell proliferation. 4)Clarification of biological mechanism, mutated α-tubulin being a poor prognostic factor in malignant lymphoma patients.The present study revealed within these two years (as follows : 1) Overexpression of mutated α-tubulin was an independent prognostic factor in gastric cancer and ovarian cancer patients, in addition to malignant lymphoma patients, on the basis of immunohistochemical results. 2)Mutated α-tubulin deeply linked to cell proliferation and expressed in G1/S and G2/M phases in the cell cycle on the basis of molecular biological results in malignant lymphoma, gastric cancer and ovarian cancer cultured cell lines.We are going to clarify the precise mechanism of mutated α-tubulin involving progression and cell proliferation of these malignant tumors by molecular biological procedures.

本研究的对象为：1)Y-49识别的突变α-微管蛋白在广谱恶性肿瘤中的过表达检测。2)过表达突变α-微管蛋白的恶性肿瘤生物学特性分析。3)细胞增殖过程中其他生物伙伴的检测。4)生物学机制的明确，α-微管蛋白突变是恶性淋巴瘤患者预后不良的因素。本研究在这两年中发现：1)免疫组化结果显示，α-微管蛋白突变过表达是胃癌和卵巢癌患者的独立预后因素，同时也是恶性淋巴瘤患者的独立预后因素。2) α-微管蛋白突变与细胞增殖密切相关，基于分子生物学结果在恶性淋巴瘤、胃癌和卵巢癌培养细胞株中表达于细胞周期G1/S和G2/M期。我们将通过分子生物学方法阐明α-微管蛋白突变参与这些恶性肿瘤进展和细胞增殖的确切机制。

项目成果

Correlation between DNA alteration and p53 and p16 protein expression in cancer cell lines.

癌细胞系中 DNA 改变与 p53 和 p16 蛋白表达之间的相关性。

• 期刊: Pathol Res Prac (in press)
• 作者: Murai Y;Hayashi S;Takahashi H;Tsuneyama K;Takano Y
• 通讯作者: Takano Y

Itoh S., et al., Takano Y: "Immunobistochemical study on overexpression of cyclooxygenase-2 in squamous cell carcinoma of the oral cavity : its importance as a prognostic predictor"Oral Oncology. 39. 829-835 (2003)

Itoh S. 等人，Takano Y：“口腔鳞状细胞癌中环氧合酶 2 过度表达的免疫组织化学研究：其作为预后预测因子的重要性”口腔肿瘤学。

Kumada T., et al., Takano Y: "Improved one-hour rapid immunostaining method using intermittent microwave irradiation : practicability based on five years application in Toyama Medical and Pharmaceutical University Hospital"Modern Pathology. (in press).

Kumada T.等人，Takano Y：“使用间歇微波照射改进的一小时快速免疫染色方法：基于富山医科大学医院五年应用的实用性”现代病理学。

Nuclear Galectin-3 Overexpression Inversely Correlates with Lymph Node Metastasis in Lung Adenocacinomas.

核半乳糖凝集素 3 过度表达与肺腺癌淋巴结转移呈负相关。

• 发表时间: 2004
• 期刊: Kitasato Med 33
• 作者: Hekmat- O.A.A.Hori R;Hayashi S;Cheng C.;Ishizawa S;Murai Y;Tsuneyama K;Matsui K;Takano Y.
• 通讯作者: Takano Y.

Improved 1-h rapid immunostaining method using intermittent microwave irradiation.

改进的使用间歇微波照射的 1 小时快速免疫染色方法。

• 发表时间: 2004
• 期刊: Mod.Pathol. 17
• 作者: Kumada T.;Tsuneyama K.;Hatta H.;Ishizawa S.Takano Y
• 通讯作者: Ishizawa S.Takano Y