Molecular mechanism of respiratory injury by suspended particulate matter in the air.

空气中悬浮颗粒物损伤呼吸系统的分子机制。

• 批准号: 13670335
• 负责人: TAKANO Yasuo
• 金额: $ 1.54万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670335/
• 关键词: Suspended particulate matter; Respiratoy injury; Alveolar type II cells; primary culture; Heavy metals; Active oxygen; 肺胞H型細胞

To investigate the interaction of suspended particulate matter (PM) in the air and respiratory health, we designed to examine cell injury of PM in vitro, using primary culture of alveolar type II cells. PM was collected using MiniVol sampler at the intersection which traffic is most heavy in Kochi city from AM 7:00 to PM 7:00 everyday for a week except Saturday and Sunday. PM was fractionated size-dependently into 3 fractions to compare the ability of inhalable particles of which the diameter is below 1 μm (PM1), from 1 to 2 μm (PM2) and above 2 μm (PM10). The average of concentrations of PM each month was 18.3±5.4mg/m^3 air for PM1, 6.1±1.9 mg/m^3 air for PM2 and 17.8±5.7mg/m^3 air for PM10. It was found by the observations of scanning electron microscopy that the form of PM1 was mosaic and spherical particles, the size was in 1 μm or thereabout, the particles did not aggregate and existed separately. While size of PM10 was irregular and various and the shape of the face was smooth and/or rough and these particles bounded and formed large aggregation. Cytotoxicity of alveolar type II cells exposed to each fraction of PM was 30.1±4.3% for control, 48.9±2.4% for PM1, 78.9±7.6 for PM2 and 105.2±4.0 for PM10, PM injured alveolar epithelial cells and the injury action of PM increased depend on particles diameter. In addition, it was found that injury components of PM exist in water soluble fraction.As PM contains several heavy metals, we also examined cytotoxicity of the heavy metals on primary cultured alveolar type II cells. The results attach to this report as a reprint of Environmental Research 89, 138-145, 2002.

为了研究空气中悬浮颗粒物（PM）与呼吸系统健康的相互作用，我们设计了体外检测PM对肺泡II型细胞的损伤。使用MiniVol采样器在高知市交通最繁忙的十字路口采集PM，采集时间为除周六、周日外的每天上午7点至下午7点。将PM按粒径分为3个组分，比较直径小于1 μm （PM1）、1 ~ 2 μm （pmm2）和大于2 μm （PM10）的可吸入颗粒物的吸入能力。月平均PM1浓度为18.3±5.4mg/m^3， PM2浓度为6.1±1.9 mg/m^3， PM10浓度为17.8±5.7mg/m^3。扫描电镜观察发现，PM1的形态为镶嵌球形颗粒，粒径在1 μm左右，颗粒不聚集，单独存在。PM10粒径不规则，形态多样，表面光滑或粗糙，颗粒结合形成较大的聚集体。PM各组分对肺泡II型细胞的细胞毒性分别为对照组（30.1±4.3%）、PM1（48.9±2.4%）、pmm2（78.9±7.6）和PM10(105.2±4.0)，PM对肺泡上皮细胞的损伤程度随PM颗粒直径的增大而增大。此外，还发现水溶性组分中存在PM的损伤成分。由于PM含有多种重金属，我们还研究了重金属对原代培养的肺泡II型细胞的细胞毒性。研究结果附于本报告，转载于《环境研究》2002年第89期，138-145页。

项目成果

Takano Y, Taguchi T, Suzuki I, Balis J.U, Yuri K: "Cytotoxicity of Heavy Metals on Primary Cultured Alveolar Type II Cells"Environmental Research. Section A, 89. 138-145 (2002)

Takano Y、Taguchi T、Suzuki I、Balis J.U、Yuri K：“重金属对原代培养的肺泡 II 型细胞的细胞毒性”环境研究。