Study on intogration of EBV genome into host DNAs of EBV positive lymphoma cells derived from SCID mouse transplantation

EBV基因组整合到SCID小鼠移植来源的EBV阳性淋巴瘤细胞宿主DNA中的研究

• 批准号: 06807018
• 负责人: TAKANO Yasuo
• 金额: $ 0.38万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807018/
• 关键词: Epstein-Barr Virus; SCID mouse; Molignant lympbhma

The investigation for integration of Epstein-Barr virus (EBV) genome into host DNAs of 30 cases of EBV positive lymphoma cells derived from SCID mouse transplantation system has been done since the beginning of 1994. DNAs extracted from these cases were cut by 3 kinds of restriction enzymesand then they were electrophoresed in 3% Nusieve GM soft agar gel. After reextraction of DNAs less than 1 kilo-base in lenght, PCR amplification was performed using the DNAs as template. Primers employed were 6 kinds for Alu-tandem repeats as sense and 3 kinds for terminal repeat of EBV as anti-sense. Single bands between 400 and 700 in length appeared in 3 cases. Direct sequencing of these DNAs revealed that DNAs apart from host DNA or EBV DNA were obtained. It was speculated to be DNAs derived from mouse DNA.Repeated examination brought same results. It passed to the middle or '96 in these condition.Another technique published by Gulley et al. (Cancer 70 : 191-195) was considered to investigate the integration of EBV DNA to host DNA.However, the study has not benn going well because specific probes for the study were not available.On the basis of accumulated knowledge and technique for EBV,we investigated the role of EBV on carcinogenesis in EBV positive gastric cancer cases and published the results at the Journal of Pathology. Moreover, a study on EBV genotype in these cases was performed and its results was submitted to the Journal of Pathology.

自1994年初以来，我们对30例来源于SCID小鼠移植系统的EB病毒（EBV）阳性淋巴瘤细胞进行了EBV基因组整合的研究。用3种限制性内切酶酶切后，用3%Nusieve GM软琼脂凝胶电泳。将长度小于1千碱基的DNA重新提取后，以该DNA为模板进行PCR扩增。所用引物为6种EB病毒末端重复序列引物和3种EB病毒末端重复序列引物。3例出现长度在400 ~ 700之间的单一条带。对这些DNA进行直接测序，结果显示，获得了除宿主DNA或EBV DNA之外的DNA。推测为小鼠DNA，反复检测，结果相同。在这种情况下，它传递到96年中期。第70章：你是谁？191-195）被认为是研究EB病毒DNA整合到宿主DNA中的方法，但由于缺乏特异性探针，研究进展并不顺利。我们研究了EB病毒在EB病毒阳性胃癌病例中致癌作用，并将结果发表在《病理学杂志》上。此外，对这些病例中的EBV基因型进行了研究，其结果已提交给病理学杂志。

项目成果

Saegusa M,Takano Y: "HPV type 16 conjunctival and junctional papillowa, dysplania and squwous cell caunoiva" Journal of Clinical Pathology. 48. 1106-1110 (1995)

Saegusa M、Takano Y：“HPV 16 型结膜和交界性乳头状瘤、发育不良和鳞状细胞 caunoiva”《临床病理学杂志》。

Saegusa M,Takano Y: "Bcl-2 expression and its correlation with apoptosis,p53 inmusoreactivity and cell proliteration in human gastic carcinomas" Journal of Cancer Research and Clinical Oncology. 121. 357-363 (1995)

Saegusa M、Takano Y：“人类胃癌中 Bcl-2 表达及其与细胞凋亡、p53 免疫反应性和细胞增殖的相关性”癌症研究和临床肿瘤学杂志。

Ikenaga M,Takano Y,et al: "Apoptosis of colon cancers assessed by in situ DNA nick-end labeling method." Pathol Int. 46. 33-37 (1995)

Ikenaga M、Takano Y 等人：“通过原位 DNA 缺口末端标记法评估结肠癌的细胞凋亡。”

Saegusa M,Takano Y: "Apoptosis in gastric carcinomas and its association with proliteration and differeutiation" Japanese Journal of Cancer Research. 86. 743-748 (1995)

Saegusa M、Takano Y：“胃癌中的细胞凋亡及其与增殖和分化的关系”日本癌症研究杂志。

Saegusa M,Takano Y,et al: "Apoptosis in gastric carcinomas and its association with proliferation and differentiation" Jpn J Cancer Res. 86. 743-748 (1995)

Saegusa M、Takano Y 等人：“胃癌中的细胞凋亡及其与增殖和分化的关系”Jpn J Cancer Res。