DEVELOPMENT OF A NEW DETECTION METHOD OF THE MINIMAL RESIDUAL TUMOR CELLS

背景：精确检测少量白血病细胞对急性白血病的诊断和治疗具有重要意义。虽然已经开发了几种携带爆炸检测程序的自动化血液学分析仪，但它们的检测灵敏度不足以超过人工眼计数法的灵敏度。方法：结合XE-2100所提供的5项细胞参数的数值信息，构建了一种新的爆炸检测程序。该爆炸多重评分程序的敏感性和特异性与最初装备在XE-2100上的“爆炸”标志程序进行了比较。结果：与“爆炸”标志程序相比，爆炸多重评分程序在不降低特异性的情况下，爆炸检测灵敏度有了很大提高。在急性髓系白血病（AML）患者的临床过程中，少量白血病母细胞的检测灵敏度优于眼计数法。结论：该blast多重评分程序的日常实际应用，必将有助于以较低的成本对AML治疗进行敏感、客观、实时的评价。

Background : For the diagnosis and therapy of acute leukemia, it is important to detect a small number of leukemic cells precisely. Although several automated hematology analyzers that carry blast-detecting programs have been developed, they do not exert sufficient detection sensitivity to exceed the sensitivity of manual eye counting method. Methods: We constructed a new blast-detecting program by combining the numerical information acquired from five cytometric parameters presented by XE-2100. The sensitivity and specificity of this blast multi-scoring program were assessed in comparison with the‘Blasts' flag program equipped originally in XE-2100.Results : The blast-detecting sensitivity was found to be highly improved in the blast multi-scoring program as compared with the‘Blasts' flag program without much reducing the specificity. A small number of leukemic myeloblasts were detected at the better sensitivity than the eye counting method in the clinical course of the patients with acute myeloid leukemia (AML).Conclusions : The daily practical use of this blast multi-scoring program will surely contribute to sensitive, objective and real-time evaluation of the treatment of AML with a low cost.