DEVELOPMENT OF A NEW DETECTION METHOD OF THE MINIMAL RESIDUAL TUMOR CELLS

最小残留肿瘤细胞检测新方法的建立

• 批准号: 15590502
• 负责人: TOHYAMA Kaoru
• 金额: $ 1.47万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590502/
• 关键词: acute myeloid leukemia; blast; minimal residual tumor cells; flow cytometry; automated hematology analyzer; 白血病細胞; 自動検出法

Background : For the diagnosis and therapy of acute leukemia, it is important to detect a small number of leukemic cells precisely. Although several automated hematology analyzers that carry blast-detecting programs have been developed, they do not exert sufficient detection sensitivity to exceed the sensitivity of manual eye counting method. Methods: We constructed a new blast-detecting program by combining the numerical information acquired from five cytometric parameters presented by XE-2100. The sensitivity and specificity of this blast multi-scoring program were assessed in comparison with the‘Blasts' flag program equipped originally in XE-2100.Results : The blast-detecting sensitivity was found to be highly improved in the blast multi-scoring program as compared with the‘Blasts' flag program without much reducing the specificity. A small number of leukemic myeloblasts were detected at the better sensitivity than the eye counting method in the clinical course of the patients with acute myeloid leukemia (AML).Conclusions : The daily practical use of this blast multi-scoring program will surely contribute to sensitive, objective and real-time evaluation of the treatment of AML with a low cost.

背景：准确检测少量白血病细胞对于急性白血病的诊断和治疗具有重要意义。尽管已经开发了几种带有爆炸检测程序的自动血液分析仪，但它们没有发挥足够的检测灵敏度来超过人工眼计数方法的灵敏度。方法：我们通过结合从 XE-2100 提供的五个细胞计数参数获得的数值信息构建了一个新的爆炸检测程序。与 XE-2100 中最初配备的“Blasts”标记程序相比，评估了该爆炸多评分程序的灵敏度和特异性。结果：与“Blasts”标记程序相比，发现爆炸多评分程序的爆炸检测灵敏度大大提高，而特异性没有太大降低。在急性髓系白血病（AML）患者的临床病程中，以比目视计数法更好的灵敏度检测到少量白血病成髓细胞。结论：这种母细胞多评分程序的日常实际使用必将有助于以较低的成本敏感、客观、实时地评估AML的治疗情况。

项目成果

Improved Detection of Minimal Acute Myeloid Leukemia Cells by the Use of the Combined Parameters of XE-2100^<TM> Hematology Analyzer

利用 XE-2100^<TM> 血液分析仪的组合参数改进微小急性髓系白血病细胞的检测

• 期刊: Cytometry (in press)
• 作者: Kaoru Tohyama 他8名

Improved Detection of Minimal Acute Myeloid Leukemia Cells by he Use of the Combined Parameters of XE-2100^<TM> Hematology Analyzer

利用XE-2100^<TM>血液分析仪的组合参数改进微小急性髓性白血病细胞的检测

• 期刊: Cytometry (in press)
• 作者: Kaoru Tohyama;et al.
• 通讯作者: et al.