DEVELOPMENT OF A NEW DETECTION METHOD OF THE MINIMAL RESIDUAL TUMOR CELLS

研究背景：准确检测少量白血病细胞对急性白血病的诊断和治疗具有重要意义。虽然已经开发了几种携带原始细胞检测程序的自动血液分析仪，但它们没有发挥足够的检测灵敏度以超过手动眼睛计数方法的灵敏度。研究方法：结合XE-2100提供的五个细胞学参数的数值信息，我们构建了一个新的爆炸检测程序。与XE-2100原有的“Blasts”flag程序进行比较，评价该程序的敏感性和特异性。结果：与“Blasts”flag程序相比，该程序在不降低特异性的情况下，显著提高了检测blast的敏感性。结论：急性髓系白血病（AML）患者的临床过程中检测到少量的白血病性成粒细胞，其灵敏度优于眼球计数法。

Background : For the diagnosis and therapy of acute leukemia, it is important to detect a small number of leukemic cells precisely. Although several automated hematology analyzers that carry blast-detecting programs have been developed, they do not exert sufficient detection sensitivity to exceed the sensitivity of manual eye counting method. Methods: We constructed a new blast-detecting program by combining the numerical information acquired from five cytometric parameters presented by XE-2100. The sensitivity and specificity of this blast multi-scoring program were assessed in comparison with the‘Blasts' flag program equipped originally in XE-2100.Results : The blast-detecting sensitivity was found to be highly improved in the blast multi-scoring program as compared with the‘Blasts' flag program without much reducing the specificity. A small number of leukemic myeloblasts were detected at the better sensitivity than the eye counting method in the clinical course of the patients with acute myeloid leukemia (AML).Conclusions : The daily practical use of this blast multi-scoring program will surely contribute to sensitive, objective and real-time evaluation of the treatment of AML with a low cost.