The mechanism of expression of gastric and intestinal character in epithelium of alimentary tract, and influence to prognosis of gastric cancer

消化道上皮胃、肠特性的表达机制及对胃癌预后的影响

• 批准号: 15590670
• 负责人: ITOH Makoto
• 金额: $ 2.24万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590670/
• 关键词: epithelial cell line; fibroblast cell line; primary culture; coculture; epithelial-mesenchymal interactions; HoxC8; HoxA5; 胃癌; 胃型形質; 腸型形質; Cdx1; Cdx2; Sox2

We established epithelial cell line from mouse glanduar stomach, and some fibroblast cell lines from mouse esophagus, forstomach, glandular stomach, jejunum and colon. By the immunofluorescence study epithelial cells were positive for tight junction protein zo-1 and cytokeratin-18 that is expressed only within simple (nonstratified) epithelium, but were not expressed specific markers for the stomach, such as MUC5AC, MUC6, pepsinogen, and H-K ATPase. All fibroblasts expressed vimentin filaments and α-SMA.To examine epithelial-mesenchymal interactions, we developed 3-dimensional coculture system using type I collagen gel. The epithelial cells formed thick branching cords that underwent progressive multifocal cavitation for 1 week when they cocultured with fibroblasts. That was the same phenomena as MontesanoR etc. observed in the renal cell line (MDCK) (1991 Cell 66: 697-711) But, formation of epithelial cells were not different even when coculture with another types of fibroblast. We speculated that fibroblasts lost their specificity during passage of culture.Hox is the homeobox gene which is necessary for development and differentiation of body, but the role of Hox gene in GI tract has not been elucidate. We investigated expression of HoxC8, HoxA5 mRNA in stomach, small. intestine and colon. RT PCR analysis revealed that Hox gene expressed only in small intestine and colon but not in stomach. We separated epithelium from mesenchyme of jejunum and investigate the expression of Hox gene separately, HoxC8 is express only in the mesenchyme of jejunum. HoxC8 must be specific and necessary for development of mesenchyme of jejunum.

我们建立了小鼠腺胃上皮细胞系和小鼠食道、胃、腺胃、空肠、结肠成纤维细胞系。通过免疫荧光研究，上皮细胞表达紧密连接蛋白ZO-1和细胞角蛋白-18，仅在简单(非复层)上皮细胞中表达，而不表达胃的特异性标志物，如MUC5AC、MUC6、胃蛋白酶原和H-K ATPase。所有成纤维细胞都表达Vimentin细丝和α-SMA。为了检测上皮-间充质的相互作用，我们建立了I型胶原凝胶的三维共培养系统。与成纤维细胞共培养1周后，上皮细胞形成粗大的分支索，并经历进行性多灶性空化1周。这与MontesanoR等在肾细胞系(MDCK)中观察到的现象相同(1991 Cell 66：697-711)，但即使与其他类型的成纤维细胞共同培养，其上皮细胞的形成也没有差异。我们推测成纤维细胞在传代培养过程中失去了其特异性。HOX基因是机体发育和分化所必需的同源盒基因，但HOX基因在胃肠道中的作用尚不清楚。我们研究了HoxC8、HoxA5在小鼠胃组织中的表达。肠道和结肠。RT-PCR分析表明，Hox基因仅在小肠和结肠中表达，在胃中不表达。我们分离了空肠上皮和间质，并分别研究了HOX基因的表达，HoxC8仅在空肠间充质中表达。HoxC8必须是空肠间充质发育所必需的特异体。

项目成果

Mizoshita, T., Inada, K., et al.: "Expression of the intestine-specific transcription factors, Cdx1 and Cdx2, correlates shift to an intestinal phenotype in gastric cancer cells"J.Cancer Res.Clin.Oncol.. 130. 29-36 (2004)

Mizoshita, T.、Inada, K. 等人：“肠道特异性转录因子 Cdx1 和 Cdx2 的表达与胃癌细胞中肠道表型的转变相关”J.Cancer Res.Clin.Oncol.. 130

Mizoshita, T., Tsukamoto, T., et al.: "Immunohistochemically detectable Cdx2 is present in intestinal phenotypic elements in early gastric cancers of both differentiated and undifferentiated types."Pathol Int.,. (In press). (2004)

Mizoshita, T.、Tsukamoto, T. 等人：“免疫组织化学可检测到的 Cdx2 存在于分化型和未分化型早期胃癌的肠道表型元件中。”Pathol Int.,.

Mizoshita, T., Tsukamoto, T., et al.: "Expression of Cdx2 and the phenotype of advanced gastric cancers : relationship with prognosis."J Cancer Res Clin Oncol. 129. 727-734 (2003)

Mizoshita, T.、Tsukamoto, T. 等人：“Cdx2 的表达和晚期胃癌的表型：与预后的关系。”J Cancer Res Clin Oncol。